共代谢条件下荧蒽降解菌的差异蛋白质组学分析

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Abstract The research object was the Bacillus cereus sp. DQ01, a highly effective Polycyclic Aromatic Hydrocarbons degrading bacteria screened by the research group. Fluoranthene and Luria-Bertani medium (LB medium) were used as the target pollutant and the co-metabolic substrate, respectively. The whole protein expression result of bacteria degrading fluoranthene in Minimal Salt Medium (MSM) and MSM-LB culture environment was compared with the isobaric tags for relative and absolute quantity (iTRAQ) technology to reveal changes in molecular metabolism level of Bacillus cereus in the process of co-metabolism. In the results, a total of 64 differentially expressed proteins were detected, consisting of 28 up-regulated proteins and 36 down-regulated proteins. It was identified that the functions of differential proteins focused on metabolism, stress response, information transmission and regulation. At the same time, compared to the results from KEGG pathway enrichment analysis, the changes in bacterial pathways were thought to relate to energy metabolism, information transmission and regulation. Compared with the differential protein expression of bacteria under co-metabolism, the key link of bacteria in the process of degrading PAHs could be speculated to be the pathway of small molecule metabolism and adaptation to the external environment.

Key words： co-metabolism comparative proteomic PAHs microbial degradation protein functional analysis

Received: 21 February 2022

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	ZHONG Qi
	WANG Hong-qi
	JIANG Ru-han
	XIAO Ya-qian
	WU Xiao-xiong

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ZHONG Qi,WANG Hong-qi,JIANG Ru-han等. Differential proteomic analysis of fluoranthene - degrading bacteria under co - metabolic conditions[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4423-4432.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I9/4423

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