青岛近岸绿潮区溶解有机质微生物可利用性

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1591 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Dissolved organic matter (DOM) in the green tide region of Qingdao coastal was studied through indoor degradation experiments, the degradation efficiency of Dissolved organic carbon(DOC) in different seawater was calculated, and the fluorescent components and their variation of colored dissolved organic matter(CDOM) in the water were analyzed. The results showed that DOC in different sites and periods influenced by green tide had all been degraded, and the degradation process accorded with the first-order degradation kinetic equation, in which about 8.93%~45.10% of DOC could be utilized by microorganisms, and the degradation rate of the samples greatly affected by the outbreak of Ulva prolifera green tide was the highest. Based on usage of three-dimensional excitation and emission matrix fluorescence spectroscopy and parallel factor analysis, it was determined that CDOM in green tide water contains three humus components (C1, C2, C3) and one protein component (C4), and the content of protein like components in all samples was significantly higher than that of each single humus component, indicating that the main component of CDOM released by Ulva prolifera green tide is protein like component. In the degradation experiment, the change of protein like components was the same as the degradation trend of DOC content, while humus like components gradually increased. Meanwhile, the results of the support vector machine weight analysis and results of correlation analysis between the fluorescence parameters of CDOM and the percentage content of bioavailable dissolved organic carbon (BDOC%) showed that compared with BDOC, C4component was positively correlated and C1component was negatively correlated, indicating that the correlation between BDOC and CDOM components is very high. It can be inferred that protein-like component and BDOC are easy to be degraded and utilized by microorganisms, and humus-like component is more closely related to RDOC(refractory dissolved organic carbon). This study revealed the changes of DOM content in water during the Ulva prolifera green tide period and its corresponding relationship with different fluorescent components, which could provide a theoretical basis for explaining the causes of secondary ecological disasters caused by Ulva prolifera green tide, and could quickly infer the content of BDOC by using CDOM components and DOC, which offers convenience for marine field investigation.

Key words： protein-like component BDOC excitation and emission matrix (EEM) Ulva prolifera

Received: 30 May 2022

PACS:

X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Zi-hao
	FENG Li-na
	ZAHNG Chuan-song
	SHI xiao-yong

Cite this article:

WANG Zi-hao,FENG Li-na,ZAHNG Chuan-song等. Microbial bioavailability of dissolved organic matter in Qingdao coastal green tide area[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(12): 5814-5823.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I12/5814

[1]	闫晓寒,文威,解莹,等.溶解性有机物特性及在国内的污染研究现状[J]. 环境科学与技术, 2020,43(6):169-178. Yan X H, Wen W, Xie Y, et al. Study on the properties of dissolved organic matter and its pollution status in China:a review[J]. Environmental Science and Technology, 2020,43(6):169-178.
[2]	陈蕾,沈超峰,陈英旭.溶解性有机质与水生生物的直接相互作用研究进展[J]. 湖泊科学, 2011,23(1):1-8. Chen L, Shen C F, Chen Y X. Direct interaction of dissolved organic matter with aquatic organisms[J]. Journal of Lake Scien-ce, 2011, 23(1):1-8.
[3]	Bendtsen J R, Lundsgaard C, Middelboe M, et al. Influence of bacterial uptake on deep-ocean dissolved organic carbon[J]. Global Biogeochemical Cycles, 2002,16(4):741-752.
[4]	Yang L, Zhuang W E, Chen T A C, et al. Unveiling the transformation and bioavailability of dissolved organic matter in contrasting hydrothermal vents using fluorescence EEM-PARAFAC[J]. Water Research, 2017,111(3):195-203.
[5]	Linda J, Colin A., Stedmon C A, et al. Changes in the composition and bioavailability of dissolved organic matter during sea ice formation[J]. Limnology and Oceanography, 2015,60(3):817-830.
[6]	Wiegner T N, Tubal R L, Mac Kenzie R A. Bioavailability and export of dissolved organic matter from a tropical river during base-and stormflow conditions[J]. Limnology and Oceanography, 2009,54(4).
[7]	Stedmon C A, Markager S. Tracing the production and degradation of autochthonous fractions of dissolved organic matter by fluorescence analysis[J]. Limnology & Oceanography, 2005,50(5):1415-1426.
[8]	朱晓敏,黄清辉,李建华.咸水藻水华期溶解有机质光谱特征变化的模拟[J]. 中国环境科学, 2009,29(1):68-72. Zhu X M, Huang Q H, Li J H. A simulation on spectral characteristics variations of dissolved organic matter during the blooms of brackish-water algae[J]. China Environmental Science, 2009,29(1):68-72.
[9]	Shanmugam P, Varunan T, Jaiganesh S, et al. Optical assessment of colored dissolved organic matter and its related parameters in dynamic coastal water systems[J]. Estuarine, Coastal and Shelf Science, 2016, 175(20):126-145.
[10]	赵巧华,秦伯强.太湖有色溶解有机质光谱吸收空间的分异特征[J]. 中国环境科学, 2008,(4):289-293. Zhao Q H, Qin B Q. Mechanisms and characteristics of spatial distribution of coloured dissolved organic matter in Taihu Lake between summer and winter[J]. China Environmental Science, 2008, (4):289-293.
[11]	Grunert B K, Mouw C B, Ciochetto A B. Characterizing CDOM spectral variability across diverse regions and spectral ranges[J]. Global Biogeochemical Cycles, 2018,32(1):57-77.
[12]	Yamashita Y, Tanoue E. Chemical characterization of protein-like fluorophores in DOM in relation to aromatic amino acids[J]. Marine Chemistry, 2003,82(3/4):255-271.
[13]	Jiao N Z, Herndl G J, Hansell D A, et al. Microbial production of recalcitrant dissolved organic matter:long-term carbon storage in the global ocean[J]. Nature Reviews Microbiology, 2010,8(8):593-599.
[14]	Yamashita Y, Jaffé R, Maie N, et al. Assessing the dynamics of dissolved organic matter (DOM) in coastal environments by excitation emission matrix fluorescence and parallel factor analysis (EEM-PARAFAC)[J]. Limnology & Oceanography, 2008,53(5):1900-1908.
[15]	Lønborg C, Álvarez-Salgado X A, Davidson K, et al. Assessing the microbial bioavailability and degradation rate constants of dissolved organic matter by fluorescence spectroscopy in the coastal upwelling system of the Ría de Vigo[J]. Marine Chemistry, 2010,119(1-4):121-129.
[16]	郭东晖,易月圆,赵磊,等.水母代谢过程中释放的溶解有机质的光谱特征[J]. 光谱学与光谱分析, 2012,32(6):1584-1587. Guo D H, Yi Y Y, Zhao L et al. Spectral characteristics of dissolved organic matter released from Jellyfish metabolism[J]. Spectroscopy and Spectral Analysis, 2012,32(6):1584-1587.
[17]	刘远,乔显亮,陈一波,等.海水养殖区排水中溶解性有机质的光化学研究[J]. 环境化学, 2016,35(8):1611-1618. Liu Y, Qiao X L, Chen Y Y, et al. Photochemical activity of dissolved organic matter in the effluent from mariculture areas[J]. Environmental Chemistry, 2016,35(8):1611-1618.
[18]	Xiao J, Wang Z L, Liu D Y, et al. Harmful macroalgal blooms (HMBs) in China's coastal water:Green and golden tides[J]. Harmful Algae, 2021,107:1-10.
[19]	Zhao J Y, Geng H X, Zhang Q C, et al. Green tides in the Yellow Sea promoted the proliferation of pelagophyte Aureococcus anophagefferens [J]. Environmental Science & Technology, 2022,56(5):3056-3064.
[20]	Barber C B, Dobkin D P, Huhdanpaa H. The quickhull algorithm for convex hulls[J]. ACM Transactions on Mathematical Software, 1996,22(4):469-483.
[21]	周倩倩.黄渤海夏秋季有色溶解有机物(CDOM)的分布特征及季节变化的研究[D]. 青岛:中国海洋大学, 2015. The Study on Distribution and Seasonal Changes of Chromophoric Dissolved Organic Matter (CDOM) in summer and autumn in the Bohai Sea[D]. Qingdao:Ocean University of China, 2015.
[22]	Li Y, Zhang Y, Li Z, et al. Characterization of colored dissolved organic matter in the northeastern South China Sea using EEMs-PARAFAC and absorption spectroscopy[J]. Journal of Sea Research, 2021,180:1-8.
[23]	Lønborg C, Álvarez-Salgado X A, Martínez-García S, et al. Stoichiometry of dissolved organic matter and the kinetics of its microbial degradation in a coastal upwelling system[J]. Aquatic Microbial Ecology, 2010,58:117-126.
[24]	Stedmon C A. Tracing dissolved organic matter in aquatic environments using a new approach to fluorescence spectroscopy[J]. Marine Chemistry, 2003,82(3/4):239-254.
[25]	Stedmon C A, Markager S. Behaviour of the optical properties of coloured dissolved organic matter under conservative mixing[J]. Estuarine Coastal & Shelf Science, 2003,57(5/6):973-979.
[26]	Lin H, Cai Y, Sun X, et al. Sources and mixing behavior of chromophoric dissolved organic matter in the Taiwan Strait[J]. Marine Chemistry, 2016,187(20):43-56.
[27]	Ren H Y, Fan T T, Yao X, et al. Investigation of the variations in dissolved organic matter properties and complexations with two typical heavy metals under the influence of biodegradation:A survey of an entire lake[J]. Science of The Total Environment, 2022,806(1):1-10.
[28]	Shutova Y, Baker A, Bridgeman J, et al. Spectroscopic characterisation of dissolved organic matter changes in drinking water treatment:from PARAFAC analysis to online monitoring wavelengths[J]. Water Research, 2014,54:159-169.
[29]	Yamashita Y, Panton A, Mahaffey C, et al. Assessing the spatial and temporal variability of dissolved organic matter in Liverpool Bay using excitation-emission matrix fluorescence and parallel factor analysis[J]. Ocean Dynamics, 2011,61(5):569-579.
[30]	Nelson N B, Siegel D A, Michaels A F. Seasonal dynamics of colored dissolved material in the Sargasso Sea[J]. Deep Sea Research Part I Oceanographic Research Papers, 1998,45(6):931-957.
[31]	Wang, X C, Chen R F, Cable J E, et al. Leaching and microbial degradation of dissolved organic matter from salt marsh plants and seagrasses[J]. Aquatic Sciences, 2014,76(4):595-609.
[32]	Wang X C, Litz L, Chen R F, et al. Release of dissolved organic matter during oxic and anoxic decomposition of salt marsh cordgrass[J]. Marine Chemistry, 2007,105(3/4):309-321.
[33]	Bianchi T S. The role of terrestrially derived organic carbon in the coastal ocean:A changing paradigm and the priming effect[J]. Proceedings of the National Academy of Sciences, 2011,108(49):19473-19481.
[34]	张婷,石晓勇,张传松,等.2008年浒苔消亡末期有机碳分布情况的初步研究[J]. 海洋环境科学, 2011,30:325-328. Zhang T, Shi X Y, Zhang C S, et al. Distribution of organic carbon in the end of the decline of Enteromorpha prolifera in 2008[J]. Marine Environmental Science, 2011,30:325-328.
[35]	Zhang T, Wang X C. Release and microbial degradation of dissolved organic matter (DOM) from the macroalgae Ulva prolifera [J]. Marine Pollution Bulletin, 2017,125(1/2):192-198.
[36]	Sulzberger B, Durisch-Kaiser E. Chemical characterization of dissolved organic matter (DOM):A prerequisite for understanding UV-induced changes of DOM absorption properties and bioavailability[J]. Aquatic Sciences, 2009,71(2):104-126.
[37]	Gan S, Wu Y, Zhang J. Bioavailability of dissolved organic carbon linked with the regional carbon cycle in the East China Sea[J]. Deep Sea Research Part II:Topical Studies in Oceanography, 2016,124:19-28.
[38]	Guo J-Q, Liang S-K, Li X-J, et al. Composition and bioavailability of dissolved organic matter in different water masses of the East China Sea[J]. Estuarine Coastal & Shelf Science, 2018,212:189-202.
[39]	Song K S, Shang Y X, Wen Z D, et al. Characterization of CDOM in saline and freshwater lakes across China using spectroscopic analysis[J]. Water Research, 2018,150(1):403-417.
[40]	甘淑钗,吴莹,鲍红艳,等.长江溶解有机质三维荧光光谱的平行因子分析[J]. 中国环境科学, 2013,33(6):1045-1052. Gan S C, Wu Y, Bao H Y, et al. Characterization of DOM (dissolved organic matter) in Yangtze River using 3-D fluorescence spectroscopy and parallel factor analysis[J]. China Environmental Science, 2013, 33(6):1045-1052.