缢蛏对潮间带原油污染的氧化应激及IBR评价

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Abstract To discuss the toxic effect which induced by intertidal crude oil pollution to organisms, antioxidant enzyme activities and lipid peroxidation in gills and visceral mass, the changes of gills structure as well as Integrated Biomarker Response (IBR) of Sinonovacula constricta exposed to different oil concentration were studied. The results showed that superoxide dismutase (SOD) activities in gills and visceral mass as well as glutathione peroxidase (GPx) activities in visceral mass appeared low concentration induction and high concentration inhibition effect in terms of dose-response. SOD induction and catalase (CAT) inhibition appeared at the same time while the rules were roughly opposite. In the time-effect, the SOD activities showed an increase-decrease-increase trend, while CAT and GPx activities showed the decrease-increase trend. The Glutathione S-transferase (GST) activities in gills showed an increase-decrease trend, the maximum value was 371.663U/mgprot. The malondialdehyde (MDA) content in the two tissues of Sinonvacula constricta increased significantly in the early stage of exposure (6h), the highest MDA content in gills and visceral mass were 5.030 and 10.705 nmol/mgprot, respectively, then gradually stabilized in the later stage. IBR results showed that biomarkers in gills were more sensitive to crude oil contamination. The crude oil exposure can deform the gills filament structure or cause their detachment. The results showed that gills of Sinonovacula constricta were more suitable as the organ for biological monitoring and evaluation of oil exposure in intertidal zone.

Key words： crude oil Sinonovacula constricta antioxidant enzyme activity IBR tissue damages

Received: 02 June 2022

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X174

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	XU Qing-xia
	PAN Yu-ying
	YANG Ting-ting
	YANG Jin-sheng
	ZHANG Meng
	CHEN Fan
	WANG Ying-ying
	TANG Zhong-wei

Cite this article:

XU Qing-xia,PAN Yu-ying,YANG Ting-ting等. Oxidative stress and IBR evaluation of Sinonovacula constricta on intertidal oil pollution[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(1): 328-340.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I1/328

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