污泥蚯蚓堆肥对染色体和质粒上耐药基因归趋的影响

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Abstract Chromosomes and plasmids mediate the vertical and horizontal transfer of antibiotic resistance genes (ARGs) in sludge, respectively, which allows ARGs to spread between parents or different bacteria strains, resulting in limited reduction of ARGs during vermicomposting. To address this issue, the effects of vermicomposting on vertical and horizontal transfer of ARGs in sludge were investigated by detecting the abundance changes of ARGs and mobile genetic elements (MGEs) on chromosomes and plasmids during vermicomposting for 20 days, with no addition of earthworms as the control. The results showed that the first 10d was the peak of ARGs transfer in sludge vermicomposting. Except for tetM gene, a significant increase in the abundance of the remaining ARGs in the vermicomposting occurred on both plasmids and chromosomes (P<0.05). Compared with the control, the gene abundances of ermF, ermB, tetX, and sul1on plasmids significantly increased by 1.02-fold, 1.97-fold, 2.43-fold, and 0.75-fold in the vermicomposting (P<0.05), while only ermB on chromosomes significantly increased (P<0.05). Compared with the control, the MGEs abundance of intI1 on plasmids significantly enriched by 1.63-fold in the vermicomposting (P<0.05), while its abundance on chromosomes was diametrically opposite, its abundance in the control was larger than vermicomposting. In the 10~20 d of composting, the abundance of MGEs and total ARGs on chromosomes and plasmids decreased in both treatments, with a faster decrease in the vermicomposting. In addition, the MGEs had a significant positive correlation (P<0.05) with ermF, ermB, and sul2 on plasmids, while no significant correlation among MGEs and all ARGs on chromosomes was recorded during vermicomposting. The redundancy analysis revealed that the changes of ARGs were related to the MGEs and environmental changes during vermicomposting, and the environmental factors such as conductivity, organic matter, ammonia and nitrate had a stronger effect on ARGs and MGEs on plasmids than those on chromosomes. This study suggests that the plasmids carrying MGEs mediated horizontal transfer is a major reason for hardly reducing ARGs in sludge vermicompost.

Key words： antibiotic resistance gene mobile genetic element excess sludge composting earthworms

Received: 01 July 2022

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X171.5

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	Articles by authors
	XU Jun-jie
	XIA Hui
	WEI Feng-yi
	CHEN Jin
	XIE Jia-chen
	HUANG Kui

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XU Jun-jie,XIA Hui,WEI Feng-yi等. Fate of antibiotic resistance genes on chromosomes and plasmids affected by earthworms during vermicomposting of dewatered sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 694-701.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/694

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