施肥模式对设施土壤抗生素及抗性基因的影响

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Abstract In this study, the surface soil (0~20cm) under different fertilization regimes was collected from a vegetable greenhouse and 83 antibiotics and 203 antibiotic resistance genes (ARGs) were analyzed. The results showed that a total of 14 antibiotics, 129 ARGs subtypes and 10 mobile genetic elements (MGEs) were detected in the soil. Tetracyclines (TCs) were the dominant antibiotics in soil, and β-lactams and multi-drug resistance genes were the dominant ARG types. Actinobacteria, Proteobacteria, Chloroflexi and Firmicutes were the main bacterial phyla in soil under different treatments. Fertilization increased the diversity and abundance of antibiotic residues and ARGs in soil, and increased the α-diversity of soil bacterial communities. The relative abundances of antibiotics, ARGs and MGEs were the highest in the treatments of manure alone and manure plus chemical fertilizer, and they were decreased by applying reduced manure. The return of rice husk to the field was helpful in controlling the pollution of antibiotics and ARGs. The selection pressure from antibiotics, changes in bacteria community and MGEs were the driving factors affecting the distribution of ARGs in greenhouse vegetable soil.

Key words： antibiotics antibiotic resistance genes antibiotic resistance mechanism microbial diversity greenhouse soil

Received: 07 June 2022

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	LIU Xiao-ying
	LI Ying
	GENG Jia-gen
	WANG Jie
	CHEN Qing
	LI Si

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LIU Xiao-ying,LI Ying,GENG Jia-gen等. Effects of fertilization regimes on antibiotics and antibiotic resistance genes in greenhouse soil[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 772-780.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/772

[1]	Shrivastava S R, Shrivastava P S, Ramasamy J, et al. World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics [J]. Journal of Medical Society, 2017,32:76-77.
[2]	Zhang Q, Ying G, Pan C, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance [J]. Environmental Science & Technology, 2015,49(11):6772-6782.
[3]	Li C, Chen J, Wang J, et al. Occurrence of antibiotics in soils and manures from greenhouse vegetable production bases of Beijing, China and an associated risk assessment [J]. Science of the Total Environment, 2015,521-522:101-107.
[4]	Zeng Q, Sun J, Zhu L, et al. Occurrence and distribution of antibiotics and resistance genes in greenhouse and open-field agricultural soils in China [J]. Chemosphere, 2019,224:900-909.
[5]	Hu X, Zhou Q, Luo Y, et al. Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China [J]. Environmental Pollution, 2010,158(9):2992-2998.
[6]	Li J, Xin Z, Zhang Y, et al. Long-term manure application increased the levels of antibiotics and antibiotic resistance genes in a greenhouse soil [J]. Applied Soil Ecology, 2017,121:193-200.
[7]	Liu P, Jia S, He X, et al. Different impacts of manure and chemical fertilizers on bacterial community structure and antibiotic resistance genes in arable soils [J]. Chemosphere, 2017,188:455-464.
[8]	张翰林,白娜玲,郑宪清,等.秸秆还田与施肥方式对稻麦轮作土壤细菌和真菌群落结构与多样性的影响 [J]. 中国生态农业学报(中英文), 2021,29(3):531-539. Zhang H L, Bai L N, Zheng X Q, et al. Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil [J]. Chinese Journal of Eco-Agriculture, 2021,29(3):531-539.
[9]	鲍陈燕,顾国平,徐秋桐,等.施肥方式对蔬菜地土壤中8种抗生素残留的影响 [J]. 农业资源与环境学报, 2014,31(4):313-318. Bao C Y, Gu G P, Xu Q T, et al. Residues of eight antibiotics in vegetable soils affected by fertilization methods [J]. Journal of Agricultural Resources and Environment, 2014,31(4):313-318.
[10]	Chen Q, An X, Li H, et al. Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil? [J]. Soil Biology and Biochemistry, 2017,114:229-237.
[11]	Liu Z P, Zhou H P, Xie W Y, et al. Long-term effects of maize straw return and manure on the microbial community in cinnamon soil in Northern China using 16S rRNA sequencing [J]. Plos One, 2021,16(4):e249884-e249899.
[12]	Hu X, Liu J, Wei D, et al. Soil bacterial communities under different long-term fertilization regimes in three locations across the black soil region of northeast China [J]. Pedosphere, 2018,28(5):751-763.
[13]	栾璐,郑洁,程梦华,等.不同秸秆还田方式对旱地红壤细菌多样性及群落结构的影响 [J]. 土壤, 2021,53(5):991-997. Luan L, Zheng J, Cheng M H, et al. Effects of different types of straw returning on bacterial diversity and community structure in dryland red soil [J]. Soils, 2021,53(5):991-997.
[14]	Li S, Shi W, You M, et al. Antibiotics in water and sediments of Danjiangkou Reservoir, China: spatiotemporal distribution and indicator screening [J]. Environmental Pollution, 2019,246:435-442.
[15]	Li S, Kuang Y, Hu J, et al. Enrichment of antibiotics in an inland lake water [J]. Environmental Research, 2020,190:110029-110037.
[16]	Zhang R, Zhang R, Li J, et al. Occurrence and distribution of antibiotics in multiple environmental media of the East River (Dongjiang) catchment, South China [J]. Environmental Science and Pollution Research, 2017,24(10):9690-9701.
[17]	Award Y M, Kim S, Abd EI-Azeem S A M, et al. Veterinary antibiotics contamination in water, sediment, and soil near a swine manure composting facility [J]. Environmental Earth Sciences, 2014, 71(3):1433-1440.
[18]	苑学霞,方丽萍,张太平,等.不同年限设施菜地土壤中重金属和抗生素污染特征 [J]. 生态环境学报, 2020,29(8):1669-1674. Yuan X X, Fang L P, Zhang T P, et al. Characteristics of heavy metals and antibiotics pollution in vegetable greenhouses after different cultivating years [J]. Ecology and Environmental Sciences, 2020,29(8): 1669-1674.
[19]	邰义萍,莫测辉,李彦文,等.东莞市蔬菜基地土壤中四环素类抗生素的含量与分布 [J]. 中国环境科学, Tai Y P, Mo C H, Li Y W, et al. Concentration and distribution of tetracycline antibiotics in soils from vegetable fields of dongguan City [J]. China Environmental Science, 2011,31(1):90-95.
[20]	梁惜梅,施震,黄小平,等.珠江口典型水产养殖区抗生素的污染特征 [J]. 生态环境学报, 2013,22(2):304-310. Liang X M, Shi Z, Huang X P, et al. Occurrence of antibiotics in typical aquaculture of the Pearl River Estuary [J]. Ecology and Environmental Sciences, 2013,22(2):304-310.
[21]	赵鑫宇,剧泽佳,陈慧,等.石家庄市土壤中喹诺酮类抗生素空间分布特征及其与微生物群落相关性 [J]. 环境科学, 2022,43(9):4684-4696. Zhao X Y, Ju Z J, Chen H, et al. Spatial distribution of quinolone antibiotics and its correlation relationship with microbial community in soil of Shijiazhuang City. [J] Environmental Science, 2022,43(9): 4684-4696.
[22]	Wei R, Ge F, Zhang L, et al. Occurrence of 13veterinary drugs in animal manure-amended soils in Eastern China [J]. Chemosphere, 2016,144:2377-2383.
[23]	Scaria J, Anupama K V, Nidheesh P V, et al. Tetracyclines in the environment: an overview on the occurrence, fate, toxicity, detection, removal methods, and sludge management [J]. Science of the Total Environment, 2021,771:145291-145313.
[24]	Pan Z, Yang S, Zhao L, et al. Temporal and spatial variability of antibiotics in agricultural soils from Huang-Huai-Hai Plain, northern China [J]. Chemosphere, 2021,272:129803.
[25]	Loftin K A, Adams C D, Meyer M T, et al. Effects of ionic strength, temperature, and pH on degradation of selected antibiotics [J]. Journal of Environmental Quality, 2008,37(2):378-386.
[26]	林雨鑫,孙明明,戴国俊,等.鸡抗球虫病的研究进展 [J]. 中国畜牧兽医, 2014,41(8):210-214. Lin Y X, Sun M M, Dai G J, et al. Effects of Lipopolysaccharide on antiviral immune response induced by porcine reproductive and respiratory syndrome virus [J]. China Animal Husbandry&Veterinary Medicine, 2014,41(8):210-214.
[27]	Hafner S C, Watanabe N, Harter T, et al. Effects of solid-liquid separation and storage on monensin attenuation in dairy waste management systems [J]. Journal of Environmental Management, 2017,190:28-34.
[28]	Žižek S, Hrženjak R, Kalcher G T, et al. Does monensin in chicken manure from poultry farms pose a threat to soil invertebrates? [J]. Chemosphere, 2011,83(4):517-523.
[29]	Sassman S A, Lee L S. Sorption and degradation in soils of veterinary ionophore antibiotics: Monensin and lasalocid [J]. Environmental Toxicology and Chemistry, 2007,26(8):1614-1621.
[30]	Donoho A L. Biochemical studies on the fate of monensin in animals and in the environment [J]. Journal of Animal Science, 1984,58(6): 1528-1539.
[31]	陈姗,许凡,张玮,等.磺胺类抗生素污染现状及其环境行为的研究进展 [J]. 环境化学, 2019,38(7):1557-1569. Chen S, Xu F, Zhang W, et al. Research progress in pollution situation and environmental behavior of Sulfonamides [J]. Environmental Chemistry, 2019,38(7):1557-1569.
[32]	Liu W, Ling N, Guo J, et al. Dynamics of the antibiotic resistome in agricultural soils amended with different sources of animal manures over three consecutive years [J]. Journal of Hazardous Materials, 2021,401:123399-123409.
[33]	Cheng J, Tang X, Cui J, et al. Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol) [J]. Science of the Total Environment, 2019,647: 853-861.
[34]	Wang F, Han W, Chen S, et al. Fifteen-year application of manure and chemical fertilizers differently impacts soil ARGs and microbial community structure [J]. Frontiers in Microbiology, 2020,11(62):1-14.
[35]	余薇薇,杨硕,杨伦,等.养殖场沼液中雌激素排放特征及去除效果研究 [J]. 中国环境科学, 2020,40(5):2103-2109. Yu W W, Yang S, Yang L, et al. Study of emission characteristics and removal efficiency of estrogens in biogas slurry at farm [J]. China Environmental Science, 2020,40(5):2103-2109.
[36]	罗芳林,刘琛,唐翔宇,等.猪粪溶解性有机物对紫色土中抗生素迁移的影响 [J]. 罗芳林,刘琛唐翔宇,等. Luo F L, Liu C, Tang X Y, et al. Effects of pig manure-derived dissolved organic matter on the transport of antibiotics in purple soils [J]. China Environmental Science, 2020,40(9):3952-3961.
[37]	Pu Q, Zhao L, Li Y, et al. Manure fertilization increase antibiotic resistance in soils from typical greenhouse vegetable production bases, China [J]. Journal of Hazardous Materials, 2020,391:122267-122274.
[38]	Peng S, Feng Y, Wang Y, et al. Prevalence of antibiotic resistance genes in soils after continually applied with different manure for 30years [J]. Journal of Hazardous Materials, 2017,340:16-25.
[39]	Zhang T, Zhang X, Ye L, et al. Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge [J]. Plos One, 2011,6(10):e26041-e26047.
[40]	Munk P, Knudsen B E, Lukjancenko O, et al. Abundance and diversity of the faecal resistome in slaughter pigs and broilers in nine European countries [J]. Nature Microbiology, 2018,3(8):898-908.
[41]	李十盛,高会,赵富强,等.水产养殖环境中抗生素抗性基因的研究进展 [J]. 中国环境科学, 2021,41(11):5314-5325. Li S S, Gao H, Zhao F Q, et al. Research progress on the occurrence and influencing factors of antibiotic resistance genes in aquaculture environment [J] China Environmental Science, 2021,41(11):5314-5325.
[42]	Zhang K, Xin R, Zhao Z, et al. Antibiotic Resistance Genes in drinking water of China: occurrence, distribution and influencing factors [J]. Ecotoxicology and Environmental Safety, 2020,188: 109837-109844.
[43]	Xie W, Yuan S, Xu M, et al. Long-term effects of manure and chemical fertilizers on soil antibiotic resistome [J]. Soil Biology and Biochemistry, 2018,122:111-119.
[44]	Zhang Y, Zheng X, Xu X, et al. Straw return promoted the simultaneous elimination of sulfamethoxazole and related antibiotic resistance genes in the paddy soil [J]. Science of the Total Environment, 2022,806:150525.
[45]	Cycoń M, Mrozik A, Piotrowska-Seget Z. Antibiotics in the soil environment—degradation and their impact on microbial activity and diversity [J]. Frontiers in Microbiology, 2019,338(10):338-382.
[46]	Zhao S C, Qiu S J, Xu X P, et al. Change in straw decomposition rate and soil microbial community composition after straw addition in different long-term fertilization soils [J]. Applied Soil Ecology, 2019, 138:123-133.
[47]	Kumar U, Kumar Nayak A, Shahid M, et al. Continuous application of inorganic and organic fertilizers over 47years in paddy soil alters the bacterial community structure and its influence on rice production [J]. Agriculture, Ecosystems & Environment, 2018,262:65-75.
[48]	Calleja-Cervantes M E, Menéndez S, Fernández-González A J, et al. Changes in soil nutrient content and bacterial community after 12 years of organic amendment application to a vineyard [J]. European Journal of Soil Science, 2015,66(4):802-812.
[49]	陈莺燕,刘文深,丁铿博,等.有机改良剂及生物炭对离子型稀土矿尾砂地生态修复的改良探究 [J]. 环境科学学报, 2018,38(12):4769-4778. Chen Y Y, Liu W S, Ding K B, et al. Effects of organic amendments and biochar on ecological remediation of ionic rare earth mine tailings [J]. Acta Scientiae Circumstantiae, 2018,38(12):4769-4778.
[50]	张庆忠,吴文良,王明新,等.秸秆还田和施氮对农田土壤呼吸的影响[J]. 生态学报, 2005,25(11):91-95. Zhang Q Z, Wu W L, Wang M X, et al. The effects of crop residue amendment and N rate on soil respiration [J]. Acta Ecologica Sinica, 2005,25(11):91-95.
[51]	Pascault N, Ranjard L, Kaisermann A, et al. Stimulation of different functional groups of bacteria by various plant residues as a driver of soil priming effect [J]. Ecosystems, 2013,16(5):810-822.
[52]	Guo Z, Wan S, Hua K, et al. Fertilization regime has a greater effect on soil microbial community structure than crop rotation and growth stage in an agroecosystem [J]. Applied Soil Ecology, 2020,149: 103510.
[53]	Wang X, Bian Q, Jiang Y, et al. Organic amendments drive shifts in microbial community structure and keystone taxa which increase C mineralization across aggregate size classes [J]. Soil Biology and Biochemistry, 2021,153:108062.
[54]	Saxena A K, Kumar M, Chakdar H, et al. Bacillus species in soil as a natural resource for plant health and nutrition [J]. Journal of Applied Mircobiology, 2020,128(6):1583-1594.
[55]	Yan M, Xu C, Huang Y, et al. Tetracyclines, sulfonamides and quinolones and their corresponding resistance genes in the Three Gorges Reservoir, China [J]. Science of the Total Environment, 2018, 631-632:840-848.
[56]	Partridge S R, Kwong S M, Firth N, et al. Mobile genetic elements associated with antimicrobial resistance [J]. Clinical Microbiology Reviews, 2018,31(4):e17-e88.
[57]	Wang B, Yan J, Li G, et al. Risk of penicillin fermentation dreg: increase of antibiotic resistance genes after soil discharge [J]. Environmental Pollution, 2020,259:113956-113963.
[58]	蔡天贵,张龙,张晋东,等.抗生素抗性基因的生态风险研究进展 [J]. 应用生态学报, 2022,33(5):1435-1440. Cai T G, Zhang L, Zhang J D, et al. Research advances in ecological risk of antibiotic resistance genes [J]. Chinese Journal of Applied Ecology, 2022,33(5):1435-1440.
[59]	Zhu B, Chen Q, Chen S, et al. Does organically produced lettuce harbor higher abundance of antibiotic resistance genes than conventionally produced? [J]. Environment International, 2017,98: 152-159.
[60]	Noguera J C, Aira M, Perez-Losada M, et al. Glucocorticoids modulate gastrointestinal microbiome in a wild bird [J]. Royal Society Open Science, 2018,5(4):171743-171750.
[61]	Coburn P S, Miller F C, Enty M A, et al. Expression of Bacillus cereus virulence-related genes in an ocular infection-related environment [J]. Microorganisms, 2020,8(4):607.