长期复合重金属污染对土壤解磷微生物的影响

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Abstract In order to further understand the changes of distribution and potential functions of phosphorus solubilizing microorganisms under long-term heavy metal pollution pressure, the response of phosphorus solubilizing microorganisms containing phoD gene or pqqE gene in the soil around a gold mine in Beijing to different levels of combined heavy metal pollution was systematically analyzed by using macrogenomics technology. It is showed that phosphorus solubilizing microorganisms was affected by heavy metals. With heavy metal concentration increasing, the relative abundance of phosphorus solubilizing microorganisms gradually decreased. Acidobacter and Actinobacteria were the dominant bacteria of phosphorus solubilizing microorganisms in heavy metal contaminated soil. Heavy metals were the most significantly related factors to the phosphorus solubilizing microbial community. The phosphorus solubilizing microorganisms were greatly affected by heavy metals As, Pb, Zn. Heavy metals were susceptibly influence Nitrospirae, Acidobacteria, Gemmatimonadete, Proteobateria, which changed the community structure. Nitrospirae was sensitive to Cr, Cu, Pb, Zn, As, Cd. The concentration of available phosphorus was significantly positively related to the diversity of phosphorus solubilizing microorganisms (P<0.05), while Alkaline phosphatase activity was significantly negatively correlated with the diversity of phosphorus solubilizing microorganisms. However, soil organic matter, total nitrogen and pH had no significant effect on the diversity of phosphorus solubilizing microorganisms in heavy metal contaminated soil. The results provided a reference for further exploring the ecological adaptation mechanism of phosphorus releasing microorganisms under long-term heavy metal pollution pressure.

Key words： phosphate solubilizing microorganisms heavy metals phoD pqqE

Received: 27 June 2022

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	Articles by authors
	LIU Rong-can
	LIU Xiao-xia
	FENG Yang
	ZHAO Qian-cheng
	HU Xue-song
	DU Yan-bin
	YU Cai-hong

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LIU Rong-can,LIU Xiao-xia,FENG Yang等. Effects of long-term combined heavy metal pollution on soil phosphate solubilizing microorganisms[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 915-926.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/915

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