锑氧化菌<i>Pseudomonas</i> sp. AO-1的分离鉴定及其对Sb(III)的氧化性能

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Abstract An antimony-oxidizing bacterium was screened from Xikuangshan by the resistance screening method and identified by molecular biology techniques. And its oxidation properties for Sb(III) and the characteristics of oxidized secondary minerals were investigated. The results showed that the antimony-oxidizing bacterium belonged to the genus Pseudomonas sp., which was named Pseudomonas sp. AO-1 (AO-1). The main factors affecting the oxidation of Sb(III) by AO-1 were solution pH, dissolved oxygen and iron/manganese oxide (elemental iron, FeCl₃ and MnO₂). In addition, AO-1 could oxidize Sb(III) under both aerobic and anoxic conditions, and the Michaelis-Menten constant and maximum oxidation rate of aerobic oxidation of Sb(III) were 393.05mmol/L and 0.271mmol/(L·min), respectively, presenting strong oxidizing ability to antimony. The combination of AO-1 and iron/manganese oxides could promote Sb(III) oxidation, and the rate of iron/manganese oxides promoting the oxidation of Sb(III) by AO-1 was in the following order: FeCl₃ > MnO₂ > elemental iron. Furthermore, the coupled oxidation of Sb(III) by AO-1 and iron/manganese oxides generated secondary minerals containing Sb(V), which accelerated Sb(III) oxidation and affected the migration and transformation of antimony in the environment. AO-1indeed exhibits satisfactory antimony oxidation performance, which is of great significance for the biogeochemical transformation of antimony and the application of soil microbial remediation.

Key words： antimony-oxidizing bacteria Pseudomonas sp. AO-1 Sb(III) oxidation iron/manganese oxide

Received: 27 June 2022

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	LONG Pei
	DENG Ren-jian
	YANG Yu
	JIN Gui-zhong
	HUANG Zhong-jie
	ZHOU Xin-he
	WANG Xi-feng
	WANG Chuang

Cite this article:

LONG Pei,DENG Ren-jian,YANG Yu等. Isolation and identification of antimony-oxidizing bacterium Pseudomonas sp. AO-1 and its oxidation properties for Sb(III)[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(2): 904-914.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I2/904

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