Fe<sub>3</sub>O<sub>4</sub>耦合厌氧微生物强化低碳源废水还原硒(IV)的研究

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Abstract In order to strengthen the removal efficiency of wastewater containing Se(IV) by anaerobic microorganisms, anaerobic sequencing batch reactor (ASBR) was used to study the effect of Fe₃O₄ on the removal of Se (IV) by anaerobic microorganisms, and the changes of selenium concentration in the effluent, selenium form and distribution, selenium reductase activity and microbial flora were analyzed. The results showed that Fe₃O₄ had no effect on the removal of Se(IV) at the wastewater with a high carbon source concentration by anaerobic microorganisms, but Fe₃O₄ could significantly improve the efficiency and rate of Se(IV) reduction at the wastewater with a low carbon source concentration by anaerobic microorganisms. Compared with the control group, the removal efficiency of Se (IV) in the magnetic group increased from (97.3±0.5)% to (98.2±0.5)%, and the maximum removal rate raised by 3.6 times. Compared with the control group, Fe₃O₄ decreased the proportion of Se (IV) and Se(0) in the supernatant and increased the proportion of Se (-II), and Fe₃O₄ strengthened the reduction process of Se(0)-Se(II) by anaerobic microorganisms. Fe₃O₄ improved increasing selenite-reduction activities, such as sulfite reductase, glutathione reductase, peripheral fumarate reductase and nitrite reductase. High-throughput sequencing results showed that Fe₃O₄ increased the relative abundance of iron-reducing bacteria Rhodococcaceae as well as the abundance of Anaerolineaceae and Dechlorobacter related to electron transport. Therefore Fe₃O₄ enhanced Se(IV) reduction by increasing reductase activities and inducing Fe(III) reduction to accelerate electron transfer. This study's results can provide technical support for the treatment of Se(IV) containing wastewater with low carbon source.

Key words： Se(IV) Fe₃O₄ anaerobic microorganism Se valence enzymatic activity dissimilatory iron reduction

Received: 20 July 2022

PACS:

X703.1

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	Articles by authors
	SUN Jie
	HOU Bo-wen
	TANG Jing-yao
	HU Liang-hui
	LI Hai-ming
	LIANG Yue-gan

Cite this article:

SUN Jie,HOU Bo-wen,TANG Jing-yao等. Enhancing Se(IV) reduction in the wastewater with a low-carbon source by Fe₃O₄ coupling with anaerobic microorganism[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(3): 1160-1165.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I3/1160

[1]	Hageman S P W, Van der Weijden R D, Weijma J, et al. Microbiological selenate to selenite conversion for selenium removal[J]. Water Research, 2013,47(7):2118-2128.
[2]	王晨辉,徐晓茵,夏四清,等.氢基质生物膜反应器去除水中硒酸盐研究[J]. 中国环境科学, 2014,34(6):1442-1447. Wang C H, Xu X Y, Xia S Q, et al. Removal of selenate from aqueous solution by hydrogen-based membrane biofilm reactor[J]. China Environmental Science, 2014,34(6):1442-1447.
[3]	Wadgaonkar S L, Mal J, Nancharaiah Y V, et al. Formation of Se(0), Te(0), and Se(0)-Te(0) nanostructures during simultaneous bioreduction of selenite and tellurite in a UASB reactor[J]. Applied Microbiology and Biotechnology, 2018,102(6):2899-2911.
[4]	Nancharaiah Y V, Lens P N L. Ecology and biotechnology of selenium-respiring bacteria[J]. Microbiology and Molecular Biology Reviews, 2015,79(1):61-80.
[5]	Wan H S, Hao O J, Kim H. Environmental factors affecting selenite reduction by a mixed culture[J]. J. Environmental Engineering, 2001,127(2):175-178.
[6]	曾涛涛,胡青,张晓玲,等.厌氧颗粒污泥处理低碳源酸性含硒、镉废水的效果及微生物群落特征[J]. 环境工程学报, 2021,15(6):2080-2090. Zeng T T, Hu Q, Zhang X L, et al. Treatment effect of acidic wastewater containing selenium and cadmium with limited carbon source by anaerobic granular sludge and the microbial community characteristics[J]. Chinese J. Environmental Engineering, 2021,15(6):2080-2090.
[7]	Santos S, Ungureanu G, Boaventura R, et al. Selenium contaminated waters:An overview of analytical methods, treatment options and recent advances in sorption methods[J]. Science of the Total Environment, 2015,521-522:246-260.
[8]	Zhang Y, Li H, Gong L, et al. Nano-sized Fe2O3/Fe3O4 facilitate anaerobic transformation of hexavalent chromium in soil-water systems[J]. Journal of Environmental Sciences, 2017,57:329-337.
[9]	Xiao L, Liu F, Liu J, et al. Nano-Fe3O4particles accelerating electromethanogenesis on an hour-long timescale in wetland soil[J]. Environmental Science:Nano, 2018,5(2):436-445.
[10]	Yang Z, Guo R, Shi X, et al. Magnetite nanoparticles enable a rapid conversion of volatile fatty acids to methane[J]. Rsc Advances, 2016,6(31):25662-25668.
[11]	Zhang Y, Amrhein C, Chang A, et al. Effect of zero-valent iron and a redox mediator on removal of selenium in agricultural drainage water[J]. Science of the Total Environment, 2008,407(1):89-96.
[12]	Xia Z C, Cheng Y Y, Kong W Q, et al. Electron shuttles alter selenite reduction pathway and redistribute formed Se(0) nanoparticles[J]. Process Biochemistry, 2016,51(3):408-413.
[13]	He Q, Yao K. Impact of alternative electron acceptors on selenium(IV) reduction by Anaeromyxobacter dehalogenans[J]. Bioresource Technology, 2011,102(3):3578-3580.
[14]	Bisswanger H. Practical enzymology[M]. Wiley-VCH, 2019:56.
[15]	Yamashita T, Hayes P. Analysis of XPS spectra of Fe2+ and Fe3+ ions in oxide materials[J]. Applied Surface Science, 2008,254(8):2441-2449.
[16]	袁永强,朱建明,刘丛强,等.芽孢杆菌SeRB-2还原亚硒酸盐的动力学研究[J]. 地球与环境, 2014,42(1):47-54. Yuan Y Q, Zhu J M, Liu C Q, et al. Study on kinetics of selenite reduction by Bacillus sp. SeRB-2[J]. Earth and Environment, 2014,42(1):47-54.
[17]	Zhang Y, Zahir, Amrhein C, et al. Application of redox mediator to accelerate selenate reduction to elemental selenium by Enterobacter taylorae[J]. J. Agricultural and Food Chemistry, 2007,55(14):5714-5717.
[18]	黎慧娟,彭静静.水稻土中铁还原菌多样性[J]. 应用生态学报, 2011, 22(10):2705-2710. Li H J, Peng J J, Phylogenetic diversity of dissimilatory Fe(III)-reducing bacteria in paddy soil[J]. Chinese Journal of Applied Ecology, 2011,22(10):2705-2710.
[19]	陈鹏程,李晓敏,李芳柏.水稻土Fe(Ⅱ)氧化耦合NO3-还原的微生物变化[J]. 中国环境科学, 2017,37(1):358-366. Chen P C, Li X M, Li F B. Shifts of microbial communities during Fe(II) oxidation coupled to nitrate reduction in paddy soil.[J]. China Environmental Science, 2017,37(1):358-366.
[20]	Yang L, Si B, Zhang Y, et al. Continuous treatment of hydrothermal liquefaction wastewater in an anaerobic biofilm reactor:Potential role of granular activated carbon[J]. J. Cleaner Production, 2020,276:122836.
[21]	Guo X, Sun C, Lin R, et al. Effects of foam nickel supplementation on anaerobic digestion:Direct interspecies electron transfer[J]. Journal of Hazardous Materials, 2020,399:122830.
[22]	Pang Y, Hu L, Wang J. Mixotrophic denitrification using pyrite and biodegradable polymer composite as electron donors[J]. Bioresource Technology, 2022,351:127011.