正交试验优化垂直潜流人工湿地实现短程硝化

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Abstract Vertical subsurface flow constructed wetland (VSFCW) was used to treat domestic wastewater and short-cut nitrification was achieved. When the temperature, pH value, dissolved oxygen (DO), and hydraulic retention time (HRT) were maintained at (30±2)℃, (8.4±0.2), (1.5±0.2) mg/L, and 12h, respectively. The removal efficiency of COD and ammonia removal efficiency (ARE) were 73.14%, 98.15%, respectively. However, nitrite accumulation rate (NAR) was 65.18%. The NAR can be improved further by adding hydroxylamine (NH₂OH) to VSFCW. A 4-factor, 5-level orthogonal experiment was designed to determine the optimal control conditions of the NH₂OH concentration, DO, temperature, and pH value. In this study, the analysis of variance (ANOVA) results revealed that the effects of NH₂OH concentration and temperature on NAR were extremely significant (P≤0.01). DO and pH value had no significant effect on NAR (P>0.05). Even if the temperature was dropped from 30℃ to 23℃, the NAR could reach 89.15% by adding 1.0mg/L NH₂OH. And the removal efficiency of COD and ARE were 75.67% and 88.76%, respectively. The abundance of Nitrosomonas increased from 1.62% to 4.06%, and the abundance of Nitrospira declined from 1.65% to 0.95%.

Key words： vertical subsurface flow constructed wetland domestic wastewater orthogonal experiment analysis of variance short-cut nitrification

Received: 05 July 2022

PACS:

X703.1

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	Articles by authors
	YAN Yuan
	MA Jiao
	DANG Hong-zhong
	WU Xin-bo
	ZENG Tian-xu
	LI Wei-wei
	CHEN Yong-zhi

Cite this article:

YAN Yuan,MA Jiao,DANG Hong-zhong等. Orthogonal experiment to optimize vertical subsurface flow constructed wetland for short-cut nitrification[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(3): 1177-1185.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I3/1177

[1]	Qu J, Dai X, Hu H Y, et al. Emerging trends and prospects for municipal wastewater management in China[J]. ACS ES&T Engineering, 2022,2(3):323-336.
[2]	Ma Y, Zhai Y, Zheng X, et al. Rural domestic wastewater treatment in constructed ditch wetlands:Effects of influent flow ratio distribution[J]. Journal of Cleaner Production, 2019,225:350-358.
[3]	Lin Z, Wang Y, Huang W, et al. Single-stage denitrifying phosphorus removal biofilter utilizing intracellular carbon source for advanced nutrient removal and phosphorus recovery[J]. Bioresource Technology, 2019,277:27-36.
[4]	李鹏峰,孙永利,隋克俭,等.我国农村污水处理现状问题分析及治理模式探讨[J]. 给水排水, 2021,57(12):65-71. Li P F, Sun Y L, Sui K J, et al. Analysis on the present situation and discussion on its treatment mode of rural sewage treatment in China[J]. Water & Wastewater Engineering, 2021,57(12):65-71.
[5]	Vymazal J. Constructed wetlands for wastewater treatment:Five decades of experience[J]. Environmental Science & Technology, 2011,45(1):61-69.
[6]	Zheng X, Zhou W, Wan R, et al. Increasing municipal wastewater BNR by using the preferred carbon source derived from kitchen wastewater to enhance phosphorus uptake and short-cut nitrification-denitrification[J]. Chemical Engineering Journal, 2018,344:556-564.
[7]	Liu X, Kim M, Nakhla G, et al. Partial nitrification-reactor configurations, and operational conditions:Performance analysis[J]. Journal of Environmental Chemical Engineering, 2020,8(4):103984.
[8]	Chang M, Liang B, Zhang K, et al. Simultaneous shortcut nitrification and denitrification in a hybrid membrane aerated biofilms reactor (H-MBfR) for nitrogen removal from low COD/N wastewater[J]. Water Research, 2022,211:118027.
[9]	Zhang N, He Y, Yi X, et al. Rapid start-up of autotrophic shortcut nitrification system in SBR and microbial community analysis[J]. Environmental Technology, 2021,42:1-13.
[10]	Xue Y, Yang F, Liu S, et al. The influence of controlling factors on the start-up and operation for partial nitrification in membrane bioreactor[J]. Bioresource Technology, 2009,100(3):1055-1060.
[11]	Balmelle B, Nguyen K M, Capdeville B, et al. Study of factors controlling nitrite build-up in biological processes for water nitrification[J]. Water Science and Technology, 1992,26(5/6):1017-1025.
[12]	Hou J, Xia L, Ma T, et al. Achieving short-cut nitrification and denitrification in modified intermittently aerated constructed wetland[J]. Bioresource Technology, 2017,232:10-17.
[13]	Tokutomi T. Operation of a nitrite-type airlift reactor at low DO concentration[J]. Water Science and Technology, 2004,49(5/6):81-88.
[14]	Wang Z, Zhang L, Zhang F, et al. Nitrite accumulation in comammox-dominated nitrification-denitrification reactors:effects of DO concentration and hydroxylamine addition[J]. Journal of hazardous materials, 2020,384:121375.
[15]	孙晓文,季诚云,杨永强,等.垂直-潮汐流人工湿地处理养猪沼液:短程硝化的实现及机理[J]. 环境工程学报, 2021,15(3):992-1005.1 Sun X W, Ji C Y, Yang Y Q, et al. Achievement and mechanism of partial nitrification in vertical-tidal flow constructed wetland treating anaerobically-digested swine wastewater[J]. Chinese Journal of Environmental Engineering, 2021,15(3):992-1005.
[16]	Zhang F, Peng Y, Sun J, et al. Ultra-low energy consumption process (PN+ Anammox) for enhanced nitrogen removal from decentralized sewage[J]. Chemical Engineering Journal, 2021,426:130769.
[17]	Zhao J, Zhao J, Xie S, et al. The role of hydroxylamine in promoting conversion from complete nitrification to partial nitrification:NO toxicity inhibition and its characteristics[J]. Bioresource Technology, 2021,319:124230.
[18]	Xu G, Xu X, Yang F, et al. Partial nitrification adjusted by hydroxylamine in aerobic granules under high DO and ambient temperature and subsequent Anammox for low C/N wastewater treatment[J]. Chemical Engineering Journal, 2012,213:338-345.
[19]	Jin C, Sun J, Chen Y, et al. Sawdust wastes-derived porous carbons for CO2 adsorption. Part 1. Optimization preparation via orthogonal experiment[J]. Separation and Purification Technology, 2021,276:119270.
[20]	Jiaqiang E, Han D, Qiu A, et al. Orthogonal experimental design of liquid-cooling structure on the cooling effect of a liquid-cooled battery thermal management system[J]. Applied Thermal Engineering, 2018,132:508-520.
[21]	刘慧春,朱开元,周江华,等.常绿水生鸢尾抗寒机理的初步研究[J]. 浙江农业科学, 2011,(1):62-65. Liu H C, Zhu K Y, Zhou J H, et al. Preliminary study on cold resistance mechanism of evergreen aquatic iris[J]. Journal of Zhejiang Agricultural Sciences, 2011,(1):62-65.
[22]	汪秀芳,许开平,叶碎高,等.四种冬季水生植物组合对富营养化水体的净化效果[J]. 生态学杂志, 2013,32(2):401-406. Wang X F, Xu K P, Ye S G, et al. Purification efficiency of four combinations of aquatic macrophytes on eutrophic water body in winter[J]. Chinese Journal of Ecology, 2013,32(2):401-406.
[23]	纪庆亮.三种水生植物氨氮耐受性和冬季净水效果研究[D]. 南京:南京林业大学, 2010. Ji Q L. NH4+-N tolerance and water purification effects in winter of three aquatic plants[D]. Nanjing:Nanjing Forestry University, 2010.
[24]	国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002. Water and wastewater monitoring analysis method[M]. 4th Edition. Beijing:China Environmental Science Press, 2002.
[25]	Cui B, Liu X, Yang Q, et al. Achieving partial denitrification through control of biofilm structure during biofilm growth in denitrifying biofilter[J]. Bioresource Technology, 2017,238:223-231.
[26]	Li H, Chi Z, Yan B. Successful start-up of the anammox process in constructed wetland microcosms:Influence of the electron acceptors on performance, microbial community, and functional genes[J]. Environmental Science and Pollution Research, 2019,26(5):5202-5209.
[27]	Spieles D J, Mitsch W J. The effects of season and hydrologic and chemical loading on nitrate retention in constructed wetlands:A comparison of low-and high-nutrient riverine systems[J]. Ecological Engineering, 1999,14(1/2):77-91.
[28]	Fan J, Zhang B, Zhang J, et al. Intermittent aeration strategy to enhance organics and nitrogen removal in subsurface flow constructed wetlands[J]. Bioresource Technology, 2013,141:117-122.
[29]	Boog J, Nivala J, Aubron T, et al. Hydraulic characterization and optimization of total nitrogen removal in an aerated vertical subsurface flow treatment wetland[J]. Bioresource Technology, 2014,162:166-174.
[30]	Saeed T, Sun G. A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands:Dependency on environmental parameters, operating conditions and supporting media[J]. Journal of Environmental Management, 2012,112:429-448.
[31]	Vohla C, Kõiv M, Bavor H J, et al. Filter materials for phosphorus removal from wastewater in treatment wetlands-A review[J]. Ecological Engineering, 2011,37(1):70-89.
[32]	Harper Jr W F, Terada A, Poly F, et al. The effect of hydroxylamine on the activity and aggregate structure of autotrophic nitrifying bioreactor cultures[J]. Biotechnology and Bioengineering, 2009,102(3):714-724.
[33]	吴启凤,丁晨雨,何腾霞,等.羟胺氧化还原酶在生物脱氮中的研究进展[J]. 微生物学通报, 2022,49(3):1120-1134. Wu Q F, Ding C Y, He T X, et al. Research progress of hydroxylamine oxidoreductase in biological denification[J]. Microbiology China, 2022,49(3):1120-1134.
[34]	李佳,李夕耀,张琼,等.投加羟胺原位恢复城市污水短程硝化-厌氧氨氧化工艺[J]. 中国环境科学, 2019,39(7):2789-2795. Li J, Li X Y, Zhang Q, et al. In-situ restoring demostic wastewater partial nitritation/anammox (PN/A) process by addition of hydroxylamine[J]. China Environmental Science, 2019,39(7):2789-2795.
[35]	Wang B, Qiao X, Hou F, et al.Pilot-scale demonstration of a novel process integrating partial nitritation with simultaneous anammox, denitrification and sludge fermentation (PN + ADSF) for nitrogen removal and sludge reduction[J]. Science of the Total Environment, 2022,815:152835.
[36]	范军辉,郝瑞霞,LIU Lei, 等.SCSC-S/Fe复合系统脱氮除磷途径及微生物群落特性[J]. 中国环境科学, 2017,37(4):1358-1365. Fan J H, Hao R X, Liu L, et al. Way of nitrogen and phosphorus removal and microbial community characteristics for SCSC-S/Fe[J]. China Environmental Science, 2017,37(4):1358-1365.
[37]	魏小涵,毕学军,尹志轩,等.温度和DO对MBBR系统硝化和反硝化的影响[J]. 中国环境科学, 2019,39(2):612-618. Wei X H, Bi X J, Yin Z X, et al. Effects of temperature and dissolved oxygen on nitrification and denification in MBBR system[J]. China Environmental Science, 2019,39(2):612-618.
[38]	张燕,周小红,陈洪斌,等.温度对强化混凝-悬浮填料床生物氧化工艺运行的影响[J]. 给水排水, 2006,(S1):27-31. Zhang Y, Zhou X H, Chen H B, et al. The running performance of combined process of CEPT-MBBR in different temperatures[J]. Water & Wastewater Engineering, 2006,(S1):27-31.
[39]	Li J, Zhang Q, Li X, et al. Rapid start-up and stable maintenance of domestic wastewater nitritation through short-term hydroxylamine addition[J]. Bioresource Technology, 2019,278:468-472.
[40]	Park S, Bae W, Chung J, et al. Empirical model of the pH dependence of the maximum specific nitrification rate[J]. Process Biochemistry, 2007,42(12):1671-1676.
[41]	陈佼,张建强,文海燕,等.羟胺抑制协同pH调控对人工快渗系统短程硝化的影响[J]. 环境科学学报, 2016,36(10):3728-3735. Chen J, Zhang J Q, Wen H Y, et al. The effect of hydroxylamine inhibition and pH control on achieving shortcut nitrification in constructed rapid infiltration system[J]. Acta Scientiae Circumstantiae, 2016,36(10):3728-3735.
[42]	Li X, Lu M, Huang Y, et al. Influence of seasonal temperature change on autotrophic nitrogen removal for mature landfill leachate treatment with high-ammonia by partial nitrification-Anammox process[J]. Journal of Environmental Sciences, 2021,102:291-300.
[43]	Peng Y, Zhu G. Biological nitrogen removal with nitrification and denitrification via nitrite pathway[J]. Applied Microbiology and Biotechnology, 2006,73(1):15-26.
[44]	Liu C, Liu D, Qi Y, et al. The effect of anaerobic-aerobic and feast-famine cultivation pattern on bacterial diversity during poly-β-hydroxybutyrate production from domestic sewage sludge[J]. Environmental Science and Pollution Research, 2016,23(13):12966-12975.
[45]	Kuypers M M M, Marchant H K, Kartal B. The microbial nitrogen-cycling network[J]. Nature Reviews Microbiology, 2018,16(5):263-276.
[46]	Sekiguchi Y, Yamada T, Hanada S, et al. Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level[J]. International Journal of Systematic and Evolutionary Microbiology, 2003,53(6):1843-1851.
[47]	Arumugam K, Bağcı C, Bessarab I, et al. Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data[J]. Microbiome, 2019,7(1):1-13.
[48]	Zhang X, Zhang N, Chen Z, et al. Long-term impact of sulfate on an autotrophic nitrogen removal system integrated partial nitrification, anammox and endogenous denitrification (PAED)[J]. Chemosphere, 2019,235:336-343.
[49]	Wang X, Zhao J, Yu D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355:560-571.
[50]	陈佼.人工快渗系统PN-ANAMMOX耦合脱氮性能及机理研究[D]. 重庆:西南交通大学, 2018. Chen J. The performance and mechanism of PN-Anammox coupling denitrification in constructed rapid infiltration system[D]. Chongqing:Southwest Jiaotong University, 2018.
[51]	陈婧.沸石序批式反应器的亚硝化及其低碳脱氮研究[D]. 广州:华南理工大学, 2019. Chen J. Low-carbon nitrogen removal based on partial nitrification of zeolite sequencing batch reactor[D]. Guangzhou:South China University of Technology, 2019.