镉耐性菌对黑麦草生长特性及镉吸收的影响

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Abstract In this study, cadmium was added to the soil, and Cd-tolerant bacteria Cdq4-2 (Enterococcus sp.) was inoculated to conduct a 40-day indoor culture experiment. Finally, the aboveground biomass, underground biomass and total biomass, chlorophyll a, chlorophyll b and total chlorophyll contents of ryegrass, the activities of antioxidant enzymes (SOD, POD) of L. perenne were determined, cadmium concentrations in ryegrass and in soil were measured to explore the effects of cadmium tolerant bacteria on the physiological and biochemical characteristics of ryegrass and the remediation efficiency of cadmium contaminated soil. Four treatments were designed:C1-Cd-contaminated soil (Cd, 4mg/kg), C1B-Cd-contaminated soil (Cd, 4mg/kg) + Cd-tolerant bacteria, C2-Cd-contaminated soil (Cd, 20mg/kg), and C2B-Cd-contaminated soil (Cd, 20mg/kg) + Cd-tolerant bacteria, and soil without Cd was used as control (CK). The results showed that the aboveground biomass, underground biomass and total biomass of L. perenne in the C2B treatment inoculated with Cdq4-2 were 39.57%, 171.88% and 50.15% higher than those in the C2 treatment without Cdq4-2, respectively. Compared with C1, the contents of chlorophyll A, chlorophyll B and total chlorophyll of L. perenne in C1B group were significantly increased by 29.81%, 29.65% and 29.77%, respectively. Compared with C1, POD activity of L. perenne in C1B was significantly increased by 150.75%, and that in C2B was significantly increased by 146.51%; The cadmium concentration of L. perenne in C1B was significantly increased by 58.29% compared with C1, and that of C2B was significantly increased by 30.53% compared with C2. The cadmium concentration of L. perenne in C2B was significantly increased by 24.25% compared with C2. The enrichment coefficient of L. perenne in C1B was significantly increased by 58.29% compared with C1, and that of C2B was significantly increased by 30.53% compared with C2. The transport coefficient of C1B is 74.84% higher than that of C1. The Cd-tolerant bacteria inoculated in the Cd-contaminated soil could enhance the photosynthesis of L. perenne, improve the antioxidant ability of L. perenne, promote the uptake, transport and accumulation of cadmium in L. perenne, so as to improve the efficiency of L. perenne remediation of cadmium contaminated soil.

Key words： cadmium-tolerant bacteria Lolium perenne cadmium pollution antioxidant stress combined remediation enrichment and transport

Received: 02 August 2022

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	SONG Lan-ping
	XU Xiao-yang
	HONG Wan-yue
	AI Yan-mei
	WANG Yang
	ZHANG Ze-kun
	ZHOU Shou-biao
	ZHOU Ji-hai

Cite this article:

SONG Lan-ping,XU Xiao-yang,HONG Wan-yue等. Effect of cadmium-tolerant bacteria on Lolium perenne growth and its cadmium enrichment[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(3): 1386-1396.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I3/1386

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