温度对好氧颗粒污泥系统污泥膨胀的影响

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Abstract To explore the effects of temperature on sludge bulking and the characteristics of microbial community structure in aerobic granular sludge system, three sequencing batch reactors were used with artificial wastewater as the influent. Illumina sequencing technology was used to analyze the microbial community structure and dynamic changes of bacteria in aerobic granular sludge system at 10℃,18℃ and 25℃,respectively.The results showed that the particle sludge settling performance of the 18℃ was good, and the SVI maintained at about 40mL/g. But under the conditions of 10℃ and 25℃, aerobic granular sludge were bulked, of which the SVI were 194.67mL/g and 100mL/g, respectively. According to the qPCR analysis, at 10℃, the abundance value of Sphaerotilus Natans was the highest among the specific filamentous bacteria, it mass propagation of this bacterium would leaded to extremely high SVI. Illumina sequencing results showed that temperature caused the significant differences of dominant species among these three systems. The predominant strains at 18℃ were Tsukamurella and unclassified_f_Comamonadaceae, this system had a good pollutant removal ability. And at 10℃, the predominant species was Sphaerotilus, which was unable to utilize complex organic matter. As for the system at 25℃, the dominant bacteria were Tsukamurella and unclassified_f_Microbacteriacea. To analyze the nitrogen removal performance of aerobic granular sludge system from the micro point of view, the FAPROTAX database was used to predict the differences in bacterial metabolic functions at different temperatures. At 18℃, the relative abundance of nitrate reduction and nitrate respiration bacteria, which were kinds of nitrogen cycling functional bacteria, were 7.81% and 7.78%, higher than those in other systems. This results matched the better nitrogen removal effect at the same temperatre.

Key words： aerobic granular sludge temperature filamentous bacteria microbial community structure bacterial metabolic function

Received: 30 August 2022

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X703

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	Articles by authors
	OU Jia-li
	GAO Chun-di
	HAN Ying-lu
	YANG Xiao-yang
	CHENG Li-yang
	PENG Yong-zhen

Cite this article:

OU Jia-li,GAO Chun-di,HAN Ying-lu等. Effect of temperature on sludge bulking in aerobic granular sludge system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1716-1723.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1716

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