生物-非生物添加剂协同促进餐饮业厨余垃圾厌氧消化——产气效能及微生物菌群动态变化

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Abstract The aim of this study was to solve the problem of unstable process induced by low methane production rate, "acid accumulation" and "ammonia inhibition" in the anaerobic digestion of floatable-oil recovered food waste (FORFW) generated from the catering industry. Based on our previous research that combined bio-abiotic addition could enhance the anaerobic digestion of food waste synergistically, a three factors-three levels orthogonal experiment of mesophilic anaerobic digestion was conducted in this study to investigate and optimize reaction parameters, including inoculum ratio, iron addition amount and yeast amount. The intrinsic microbial mechanism of synergistic promotion of bio-abiotic additives in FORFW methanogenesis was elucidated by the high throughput sequencing technique. It was found that the influence of three parameters on the methane yield of FORFW anaerobic digestion: inoculum ratio > yeast amount > iron addition amount. The optimal condition were inoculation ratio of 1.5, iron addition of 0.5% (w/w) and activated yeast of 3%(w/w). Under the optimal condition, the VS removal rate of FORFW was 40.1%, and the accumulative methane yield was 237.5mL/g VS. The relative abundance of genera Methanosaeta, Syntrophomonas, and Syner-01 increased significantly in the system, resulting in efficient conversion of lactic acid-derived propionic acid. Genus Methanosaeta interacted with Anaerolineaceae in syntrophic methanogenesis, Syntrophomonas was involved in butyric acid oxidation process, while Syner-01 participated in acetic acid oxidation and amino acid oxidation. Thus, the methane yield of the FORFW generated from the catering industry was ensured. The research results will provide scientific guidance for further practical application and process control.

Key words： anaerobic digestion food waste catering industry bio-abiotic additives orthogonal test microbial dynamics

Received: 23 September 2022

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X705

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	Articles by authors
	HU Jin-hui
	MENG Wei
	XIA Yi
	YANG Zheng
	ZHANG Xin-yu
	QI Guang-xia
	REN Lian-hai

Cite this article:

HU Jin-hui,MENG Wei,XIA Yi等. A synergistic promotion during anaerobic digestion of food waste generated from catering industry by the bio-abiotic additives——Biogas production efficiency and corresponding microbial dynamic changes[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1757-1764.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1757

[1]	王向会,李广魏,孟虹,等.国内外餐厨垃圾处理状况概述[J]. 环境卫生工程, 2005,(2):41-43. Wang X H, Li G W, Meng H, et al. Discussion on treatment status of food residue[J]. Environmental Sanitation Engineering, 2005,(2):41-43.
[2]	李晶,李宇聪.餐厨垃圾处理工艺及装置发展现状与展望[J]. 中国食品, 2021,(19):138-139. Li J, Li Y C. Development status and prospect of kitchen waste treatment technology and equipment[J]. China Food, 2021,(19):138-139.
[3]	李亚亚,唐婧.餐厨垃圾厌氧消化性能提高研究现状与展望[J]. 农业与技术, 2021,41(11):110-113. Li Y Y, Tang J. Research status and prospect of improving anaerobic digestion performance of kitchen waste[J]. Agriculture and Technology, 2021,41(11):110-113.
[4]	Kurade M B, Saha S, Salama E S, et al. Acetoclastic methanogenesis led by Methanosarcina in anaerobic co-digestion of fats, oil and grease for enhanced production of methane[J]. Bioresource Technology, 2019,272:351-359.
[5]	张靖雪,李盼盼,于洋,等.基于固液分离预处理的餐厨垃圾厌氧发酵[J]. 中国环境科学, 2022,42(3):1252-1258. Zhang J X, Li P P, Yu Y, Zeng S, Yu R, Yang G P. Study on anaerobic digestion of kitchen waste based on solid-liquid separation pretreatment[J]. China Environmental Science, 2022,42(3):1252-1258.
[6]	Meng X, Zhang Y, Li Q, et al. Adding Fe0powder to enhance the anaerobic conversion of propionate to acetate[J]. Biochemical Engineering Journal, 2013,73:80-85.
[7]	Ko J H, Wang N, Yuan T, et al. Effect of nickel-containing activated carbon on food waste anaerobic digestion[J]. Bioresource Technology, 2018,266:516-523.
[8]	Chen X, Yan W, Sheng K, et al. Comparison of high-solids to liquid anaerobic co-digestion of food waste and green waste[J]. Bioresource Technology, 2014,154:215-221.
[9]	李海红,巴琦玥,闫志英,等.不同原料厌氧发酵及其微生物种群的研究[J]. 中国环境科学, 2015,35(5):1449-1457. Li H H, Ba Q Y, Yan Z Y, Liu X F. Studies on microbial community of different materials and anaerobic fermentation[J]. China Environmental Science, 2015,35(5):1449-1457.
[10]	Kong X, Wei Y, Xu S, et al. Inhibiting excessive acidification using zero-valent iron in anaerobic digestion of food waste at high organic load rates[J]. Bioresource Technology, 2016,211:65-71.
[11]	Barua S, Dhar B R. Advances towards understanding and engineering direct interspecies electron transfer in anaerobic digestion[J]. Bioresource Technology, 2017,244(Pt 1):698-707.
[12]	Yang Y, Yang F, Huang W, et al. Enhanced anaerobic digestion of ammonia-rich swine manure by zero-valent iron:With special focus on the enhancement effect on hydrogenotrophic methanogenesis activity[J]. Bioresource Technology, 2018,270:172-179.
[13]	Kong X, Yu S, Xu S, et al. Effect of Fe0 addition on volatile fatty acids evolution on anaerobic digestion at high organic loading rates[J]. Waste Management, 2018,71:719-727.
[14]	Zhang Y, Feng Y, Yu Q, et al. Enhanced high-solids anaerobic digestion of waste activated sludge by the addition of scrap iron[J]. Bioresource Technology, 2014,159:297-304.
[15]	Baek G, Kim J, Lee C. Influence of ferric oxyhydroxide addition on biomethanation of waste activated sludge in a continuous reactor[J]. Bioresource Technology, 2014,166:596-601.
[16]	Kim S J, Park S J, Cha I T, et al. Metabolic versatility of toluene-degrading, iron-reducing bacteria in tidal flat sediment, characterized by stable isotope probing-based metagenomic analysis[J]. Environmental Microbiology, 2014,16(1):189-204.
[17]	Ye J, Hu A, Ren G, et al. Enhancing sludge methanogenesis with improved redox activity of extracellular polymeric substances by hematite in red mud[J]. Water Research, 2018,134:54-62.
[18]	Rotaru A E, Shrestha P M, Liu F, et al. A new model for electron flow during anaerobic digestion:direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. Energy & Environmental Science, 2014,7(1):408-415.
[19]	Coates J D, Cole K A, Michaelidou U, et al. Biological control of hog waste odor through stimulated microbial Fe(III) reduction[J]. Applied and Environmental Microbiology, 2005,71(8):4728-4735.
[20]	Wang M, Zhao Z, Niu J, et al. Potential of crystalline and amorphous ferric oxides for biostimulation of anaerobic digestion[J]. ACS Sustainable Chemistry & Engineering, 2018,7(1):697-708.
[21]	Shen L, Zhao Q, Wu X, et al. Interspecies electron transfer in syntrophic methanogenic consortia:From cultures to bioreactors[J]. Renewable and Sustainable Energy Reviews, 2016,54(C):1358-1367.
[22]	Wang R H, Gao J, Li M, et al. The complete chloroplast genome sequences of Barnardia japonica (Thunb.) Schult. and Schult.f[J]. Mitochondrial DNA Part B, 2018,3(2):697-698.
[23]	Jin C, Zhao Z, Zhao Y, et al. Effects of yeast on bacterial community in kitchen waste anaerobic fermentation system[J]. 4th International Conference on Bioinformatics and Biomedical Engineering:, IEEE, 2010.
[24]	花俊杰.餐厨垃圾酸败成分乳酸定向降解强化电子传递产甲烷研究[D]. 杭州:浙江大学, 2021. Hua J J. Directional degradation of lactic acid in soured food waste to promote methane production with enhanced electron transport[D]. Hangzhou:Zhejiang University, 2021.
[25]	赵振焕,金春姬,张鹏,等.酵母菌对厨余垃圾厌氧发酵产乙酸的影响[J]. 环境工程学报, 2009,3(10):1885-1888. Zhao Z H, Jin C J, Zhang P, et al. Influence of yeast on anaerobic fermentation of kitchen waste for producing acetic acid[J]. Journal of Environmental Engineering, 2009,3(10):1885-1888.
[26]	Shen Y, Zhao X Q, Ge X M, et al. Metabolic flux and cell cycle analysis indicating new mechanism underlying process oscillation in continuous ethanol fermentation with Saccharomyces cerevisiae under VHG conditions[J]. Biotechnology Advances, 2009,27(6):1118-1123.
[27]	于淼.强化乙醇型发酵的餐厨垃圾厌氧消化性能及调控机制研究[D]. 北京:北京科技大学, 2019. Yu M. Enhanced Ethanol-type fermentation of food waste anaerobic digestion system:Performance and regulation mechanism[D]. Beijing:University of Science and Technology Beijing, 2019.
[28]	Wu C, Wang Q, Yu M, et al. Effect of ethanol pre-fermentation and inoculum-to-substrate ratio on methane yield from food waste and distillers' grains[J]. Applied Energy, 2015,155:846-853.
[29]	Boris M. Ammonia assimilation by Saccharomyces cerevisiae[J]. Eukaryotic Cell, 2003,2(5):827-829.
[30]	Holmes A R, Collings A, Farnden K J, et al. Ammonium assimilation by Candida albicans and other yeasts:evidence for activity of glutamate synthase[J]. Journal of General Microbiology, 1989,135(6):1423-1430.
[31]	祁光霞,孟伟,查金,等.一种餐厨垃圾干式厌氧发酵快速启动及稳定运行方法:中国, 110257437B[P]. 2021-07-30. Qi G X, Meng W, Zha J, et al. A fast start-up and stable operation method of dry anaerobic fermentation of kitchen waste:China, 110257437B[P]. 2021-07-30.
[32]	Cheng J, Hua J, Kang T, et al. Nanoscale zero-valent iron improved lactic acid degradation to produce methane through anaerobic digestion[J]. Bioresource Technology, 2020,317:124013.
[33]	Perego P, Fabiano B, Ponzano G P, et al. Experimental study of hydrogen kinetics from agroindustrial by-product:optimal conditions for production and fuel cell feeding[J]. Bioprocess Engineering, 1998,19(3):205-211.
[34]	Taguchi F, Mizukami N, Hasegawa K, et al. Microbial conversion of arabinose and xylose to hydrogen by a newly isolated Clostridium sp. No. 2[J]. Canadian Journal of Microbiology, 1994,40(3):228-233.
[35]	Feng K, Li H, Zheng C. Shifting product spectrum by pH adjustment during long-term continuous anaerobic fermentation of food waste[J]. Bioresource Technology, 2018,270:180-188.
[36]	Feng K, Wang Q, Li H, et al. Microbial mechanism of enhancing methane production from anaerobic digestion of food waste via phase separation and pH control[J]. Journal of Environmental Management, 2021,288:112460.
[37]	常城,明磊强,牟云飞,等.厨余垃圾与污泥厌氧发酵产甲烷的协同作用[J]. 中国环境科学, 2022,42(3):1259-1266. Chang C, Ming L Q, Mou Y F, et al. Synergistic effect of kitchen waste and sludge anaerobic fermentation for methane production[J]. China Environmental Science, 2022,42(3):1259-1266.
[38]	Chen S, Dong X. Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater[J]. International Journal of Systematic and Evolutionary Microbiology, 2005,55,(Pt 6):2257-2261.
[39]	Lemos L N, Medeiros J D, Andreote F D, et al. Genomic signatures and co-occurrence patterns of the ultra-small Saccharimonadia (phylum CPR/Patescibacteria) suggest a symbiotic lifestyle[J]. Molecular Ecology, 2019,28(18):4259-4271.
[40]	Vrieze J D, Gildemyn S, Vargas R V, et al. Inoculum selection is crucial to ensure operational stability in anaerobic digestion[J]. Applied Microbiology and Biotechnology, 2015,99(1):189-199.
[41]	Castro R S, Acosta H O M, Vargas J A, et al. Active prokaryotic population dynamics exhibit high correlation to reactor performance during methane production from acid hydrolysates of Agave tequilana var. azul bagasse[J]. Journal of Applied Microbiology, 2019,126(5):1618-1630.
[42]	Sousa D Z, Pereira M A, Alves J I, et al. Anaerobic microbial LCFA degradation in bioreactors[J]. Water Science and Technology, 2008, 57(3):439-444.
[43]	Wang J J, Ji Xu L Z, Huang B C, et al. Multiple electron acceptor-mediated sulfur autotrophic denitrification:Nitrogen source competition, long-term performance and microbial community evolution[J]. Bioresource Technology, 2021,329:124918.
[44]	Lv Z, Chen Z, Chen X, et al. Effects of various feedstocks on isotope fractionation of biogas and microbial community structure during anaerobic digestion[J]. Waste Management, 2019,84:211-219.
[45]	Puengrang P, Suraraksa B, Prommeenate P, et al. Diverse microbial community profiles of propionate-degrading cultures derived from different sludge sources of anaerobic wastewater treatment plants[J]. Microorganisms, 2020,8(2):277.
[46]	Zhang Y, Feng Y, Yu Q, et al. Enhanced high-solids anaerobic digestion of waste activated sludge by the addition of scrap iron[J]. Bioresource Technology, 2014,159:297-304.