寒旱地区填埋场调蓄池新鲜渗滤液稳定化影响因素

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Abstract To develop a viable and simple method to realize the bio-stabilization of landfill leachate in cold and arid regions, this experiment chose three kinds of leachate representing those with characteristics at different landfill ages. According to a 4-factor, and 3-level orthogonal rule, the study assessed the leachate concentration (3.8, 15.4, 64.0g-COD/L), sludge inoculation quantity (1, 2, 4g VS/L), agitation frequency (0, 2, 4 times/h), and temperature (10, 20, 30℃) on four aspects relating to landfill leachate bio-stabilization in the anaerobic environment of simulated regulating ponds. The findings demonstrated that the leachate concentration and temperature were the primary determinants of bio-stabilization. The lowest concentration (3.8g-COD/L) and highest temperature (30℃) condition had the shortest bio-stabilization time (58d). Analysis of the microbial community structure of each test group revealed that the bacterial and archaeal community structure were similar in each test group at low and medium concentrations (3.8 and 15.4g-COD/L), the acetoclastic methanogenesis pathway of Methanosaeta franchise dominated the methanogenesis route; while the archaeal and bacterial communities evolved significantly during the bio-stabilization process at high concentrations (64.0g-COD/L) and 30℃, and the hydrogenotrophic methanogenic bacteria Methanofolli and Methanoculleus were gradually enriched and the abundance of the phylum Firmicutes, which is rich in the genus relating to syntrophic acetate-oxidising Bacteria, increased to more than 80%, and the anaerobic methanogenesis was dominated by the tandem pathway of syntrophic acetate oxidation and hydrogenotrophic methanogenesis. Based on the analysis of the test results, it is feasible to stabilize leachate at landfills in cold and arid regions by using regulating ponds under the condition that the capacity of regulating ponds can accommodate a half-year leachate generation.

Key words： cold and arid area regulating ponds leachate anaerobic biodegradation microbial community structure

Received: 11 August 2022

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X705

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	Articles by authors
	SHU Yin-hui
	SHAO Li-ming
	DENG Ying-tao
	Lü Fan
	ZHANG Hua
	HE Pin-jing

Cite this article:

SHU Yin-hui,SHAO Li-ming,DENG Ying-tao等. The influencing factors of the stabilization of fresh leachate from landfill regulating ponds in cold and arid regions[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1765-1772.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1765

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