PM<sub>2.5</sub>典型组分诱导MRC-5细胞衰老效应及其机制研究

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Abstract To investigate the effects and mechanisms of PM_2.5 and its typical components on cellular senescence in lung tissue, human fetal lung fibroblast cells (MRC-5) were exposed to SRM1649a as PM_2.5 standard, carbon black FW200 as the core particle, and benzo[a]pyrene (B[a]P) as the main organic component, respectively. The experiments were conducted to assess the cellular senescence of MRC-5 cells by measuring SA-β-Gal activity and clarify the mechanism of cellular senescence induced by intracellular reactive oxygen levels, DNA damage and mitochondrial membrane potential. It was showed that the treatment of SRM1649a and B[a]P significantly increased the proportion of cellular senescence in MRC-5 cells with no obvious effect on cellular viability, whereas cellular senescence was not remarkably changed in the FW200-exposed group. Mechanism studies indicated that FW200 treatment caused only DNA damage rather than cellular senescence, while exposure to B[a]P induced cellular senescence by triggering changes in ROS levels. Furthermore, the mixture SRM1649a could lead to DNA double-strand breaks and decrease the mitochondrial membrane potential, which induced cellular senescence in terms of DNA damage and mitochondrial dysfunction. These results suggested that the toxic effects induced by the main components of PM_2.5 were different, and synergistic promoting effects are manifested in PM_2.5 as the mixture.

Key words： PM_2.5 typical component cellular senescence DNA damage

Received: 28 July 2022

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X171

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	XU Xiao-an
	XU Sheng-min
	WU Li-jun

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XU Xiao-an,XU Sheng-min,WU Li-jun. Effects and mechanisms of cellular senescence induced by typical PM_2.5 components in MRC-5 cells[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1967-1977.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1967

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