不同形态微塑料和汞对斑马鱼胚胎发育毒性联合作用研究

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Abstract To explore the toxicity of microplastics with different shapes to organisms, the toxicity effects of polyethylene terephthalate (PET) granules (approximately 70~250μm in diameter) and fibers (approximately 3~5mm in length and 20μm in diameter) combined with heavy metal mercury (Hg) on zebrafish embryo development were compared in the present study. The results showed that the single exposure of Hg to zebrafish embryos resulted in embryonic development toxicity (such as blood flow development disorder, cardiac relaxation, and reduced hatching rate), pericardial sac edema, and tail malformations. The malformation rates at 48 and 72h were as high as 31.3% and 91.7%, respectively. Fibrous (f-PET) and granular (p-PET) microplastics could reduce the embryonic toxicity of Hg. From the data of metabolomics, the disorder effects of glucose metabolism and amino acid metabolism caused by Hg on zebrafish larvae were significantly reduced in combined exposure of microplastics and Hg (P< 0.01). However, due to the difference in the adsorption capacity and process of Hg, p-PET significantly alleviated the 24-h blood flow disorder effect caused by Hg, while it was not as significant as f-PET in reducing the toxicity of Hg to zebrafish embryos in the later period of exposure. Thus, the combined toxic effect of microplastics and Hg was related to the shape of microplastics.

Key words： microplastics shape mercury zebrafish embryo joint toxicity

Received: 16 August 2022

PACS:

X171

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	Articles by authors
	SHI Yan-song
	WANG Jing
	CUI Wen-jie
	WANG Yu-di
	ZHANG Hai-hong
	DUAN Zheng-hua

Cite this article:

SHI Yan-song,WANG Jing,CUI Wen-jie等. The combined development toxicity of microplastics with different shapes and mercury on Zebrafish embryos[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1978-1984.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1978

[1]	Ruangpanupan N, Ussawarujikulchai A, Prapagdee B, et al. Microplastics in the surface seawater of Bandon Bay, Gulf of Thailand[J]. Marine Pollution Bulletin, 2022,179:113664.
[2]	Jiang Y, Yang F, Hassan Kazmis S U, et al. A review of microplastic pollution in seawater, sediments and organisms of the Chinese coastal and marginal seas[J]. Chemosphere, 2022,286:131677.
[3]	Wang S, Chen H, Zhou X, et al. Microplastic abundance, distribution and composition in the mid-west Pacific Ocean[J]. Environmental Pollution, 2020,264:114125.
[4]	Zheng Y, Li J, Cao W, et al. Distribution characteristics of microplastics in the seawater and sediment:A case study in Jiaozhou bay, China[J]. Science of the Total Environment, 2019,674:27-35.
[5]	Zhang L, Sun J, Zhang Z, et al. Polyethylene terephthalate microplastic fibers increase the release of extracellular antibiotic resistance genes during sewage sludge anaerobic digestion[J]. Water Research, 2022,217:118426.
[6]	Gaylarde C, Baptista N J, Da F E. Plastic microfibre pollution:How important is clothes' laundering?[J]. Heliyon, 2021,7(5):7105-7105.
[7]	Matjašič T, Simčič T, Kanduč T, et al. Presence of polyethylene terephthalate (PET) fibers in hyporheic zone alters colonization patterns and seasonal dynamics of biofilm metabolic functioning[J]. Water Research, 2021,203:117455.
[8]	Rebelein A, Int-Veen I, Kammann U, et al. Microplastic fibers-underestimated threat to aquatic organisms?[J] Science of the Total Environment, 2021,777:146045.
[9]	Bunge A, Kammann U, Scharsack J P. Exposure to microplastic fibers does not change fish early life stage development of three-spined sticklebacks (Gasterosteus aculeatus)[J]. Microplastics and Nanoplastics, 2021,1(1):15.
[10]	Zhao Y, Qiao R, Zhang S, et al. Metabolomic profiling reveals the intestinal toxicity of different length of microplastic fibers on zebrafish (Danio rerio)[J]. Journal of Hazardous Materials, 2021,403:123663.
[11]	Jemec A, Horvat P, Kunej U, et al. Uptake and effects of microplastic textile fibers on freshwater crustacean daphnia magna[J]. Environmental Pollution, 2016,219:201-209.
[12]	Li Q, Sun C, Wang Y, et al. Fusion of microplastics into the mussel byssus[J]. Environmental Pollution, 2019,252:420-426.
[13]	Ziajahromi S, Kumar A, Neale P A, et al. Impact of microplastic beads and fibers on waterflea (Ceriodaphnia dubia) survival, growth, and reproduction:Implications of single and mixture exposures[J]. Environmental Science & Technology, 2017,51(22):13397-13406.
[14]	Duan Z, Xing Y, Feng Z, et al. Hepatotoxicity of benzotriazole and its effect on the cadmium induced toxicity in zebrafish Danio rerio[J]. Environmental Pollution, 2017,224:706-713.
[15]	Kristofco L A, Haddad S P, Chambliss C K, et al. Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish[J]. Environmental Toxicology and Chemistry, 2018,37(4):1175-1181.
[16]	Qiao R, Deng Y, Zhang S, et al. Accumulation of different shapes of microplastics initiates intestinal injury and gut microbiota dysbiosis in the gut of zebrafish[J]. Chemosphere, 2019,236:124334.
[17]	Choi J S, Jung Y J, Hong N H, et al. Toxicological effects of irregularly shaped and spherical microplastics in a marine teleost, the sheepshead minnow (Cyprinodon variegatus)[J]. Marine Pollution Bulletin, 2018,129(1):231-240.
[18]	Zhang Y, Wolosker M B, Zhao Y, et al. Exposure to microplastics cause gut damage, locomotor dysfunction, epigenetic silencing, and aggravate cadmium (Cd) toxicity in Drosophila[J]. Science of the Total Environment, 2020,744:140979.
[19]	范秀磊,常卓恒,邹晔锋,等.可降解微塑料对铜和锌离子的吸附解吸特性[J]. 中国环境科学, 2021,41(5):2141-2150. Fan X L, Chang Z H, Zou Y F, et al. Adsorption and desorption properties of degradable microplastic for Cu2+ and Zn2+[J]. China Environmental Science, 2021,41(5):2141-2150.
[20]	付东东,张琼洁,范正权,等.微米级聚苯乙烯对铜的吸附特性[J]. 中国环境科学, 2019,39(11):4769-4775. Fu D D, Zhang Q J, Fan Z Q, et al. Adsorption characteristics of copper ions on polystyrene microplastics[J]. China Environmental Science, 2019,39(11):4769-4775.
[21]	Qin L, Duan Z, Cheng H, et al. Size-dependent impact of polystyrene microplastics on the toxicity of cadmium through altering neutrophil expression and metabolic regulation in zebrafish larvae[J]. Environmental Pollution, 2021,291:118169.
[22]	Wang J, Wu J, Cheng H, et al. Polystyrene microplastics inhibit the neurodevelopmental toxicity of mercury in zebrafish (Danio rerio) larvae with size-dependent effects[J]. Environment Pollution, 2022, 314:120216.
[23]	Zhang R, Wang M, Chen X, et al. Combined toxicity of microplastics and cadmium on the zebrafish embryos (Danio rerio)[J]. Science of the Total Environment, 2020,743:140638.
[24]	Barboza L G A, Vieira L R, Guilhermino L. Single and combined effects of microplastics and mercury on juveniles of the european seabass (Dicentrarchus labrax):Changes in behavioural responses and reduction of swimming velocity and resistance time[J]. Environmental Pollution, 2018,236:1014-1019.
[25]	Cheng H, Feng Y, Duan Z, et al. Toxicities of microplastic fibers and granules on the development of zebrafish embryos and their combined effects with cadmium[J]. Chemosphere, 2021,269:128677.
[26]	Chang Y, Lee W Y, Lin Y J, et al. Mercury (ii) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision[J]. Aquatic Toxicology, 2017,192:97-104.
[27]	Abu Bakar N, Mohd Sata N S A, Ramlan N F, et al. Evaluation of the neurotoxic effects of chronic embryonic exposure with inorganic mercury on motor and anxiety-like responses in zebrafish (Danio rerio) larvae[J]. Neurotoxicology and Teratology, 2017,59:53-61.
[28]	申雪娇,张健宁,刘小莉,等.汞对海洋双壳贝类毒性效应研究进展[J]. 山东农业科学, 2018,50(3):148-153. Shen X J, Zhao J N, Liu X L, et al. Research progress of toxic effects of mercury on marine bivalves[J]. Shandong Agricultural Sciences, 2018,50(3):148-153.
[29]	熊飞,黄庆辰,何玉虹,等.微塑料污染现状及其毒性效应和机制研究进展[J]. 生态毒理学报, 2021,16(5):211-220. Xiong F, Huang Q C, He Y H, et al. Reserch progress on current status of microplastics pollution an its toxic effects and mechanisms[J]. Asian Journal of Ecotoxicology, 2021,16(5):211-220.
[30]	Lu K, Qiao R, An H, et al. Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio)[J]. Chemosphere, 2018,202:514-520.
[31]	Wang Y, Hamid N, Deng S, et al Individual and combined toxicogenetic effects of microplastics and heavy metals (Cd, Pb, and Zn) perturb gut microbiota homeostasis and gonadal development in marine medaka (Oryzias melastigma)[J]. Journal of Hazardous Materials, 2020,397:122795.
[32]	Duan Z, Duan X, Zhao S, et al. Barrier function of zebrafish embryonic chorions against microplastics and nanoplastics and its impact on embryo development[J]. Journal of Hazardous Materials, 2020,395:122621.
[33]	段鑫越,关文玲,程昊东,等.胚胎绒毛膜对微塑料颗粒与镉联合作用的影响[J]. 中国环境科学, 2021,41(3):1422-1428. Duan X Y, Guan W L, Cheng H D, et al. Effect of chorionic villi on the combination action of microplastic particles and cadmium[J]. China Environmental Science, 2021,41(3):1422-1428.
[34]	Skdokur E, Belivermis M, Sezer N, et al. Effects of microplastics and mercury on manila clam Ruditapes philippinarum:Feeding rate, immunomodulation, histopathology and oxidative stress[J]. Environmental Pollution, 2020,262:114247.
[35]	Cheng H, Duan Z, Wu Y, et al. Immunotoxicity responses to polystyrene nanoplastics and their related mechanisms in the liver of zebrafish (Danio rerio) larvae[J]. Environmental International, 2022, 161:107128.
[36]	Saravia J, Raynor J L, Chapman N M, et al. Signaling networks in immunometabolism[J]. Cell Research, 2020,30(4):328-342.
[37]	Razak M A, Begum P S, Viswanath B, et al. Multifarious beneficial effect of nonessential amino acid, glycine:A review[J]. Oxidative Medicine and Cellular Longevity, 2017,2017:1716701.
[38]	Isigaki M, Ito A., Hara R, et al. Development of an amino acid sequence-dependent analytical method for peptides using near-infrared spectroscopy[J]. The Analyst, 2022,147(16):3634-3642.
[39]	Ishtiyaq A, Imtiaz I, Shabihul F, et al. Role of branched-chain amino acids on growth, physiology and metabolism of different fish species:A review[J]. Aquaculture Nutrition, 2021,27(5):207-211.
[40]	Qiu Y, Zhang Z, Zhang T, et al. Sulfide modifies physicochemical properties and mercury adsorption of microplastics[J]. Science of the Total Environment, 2022,157802.
[41]	Chubarenko I, Bagaev A, Zobkov M, et al. On some physical and dynamical properties of microplastic particles in marine environment[J]. Marine Pollution Bulletin, 2016,108(1):105-112.
[42]	Qiao R, Deng Y, Zhang S, et al. Accumulation of different shapes of microplastics initiates intestinal injury and gut microbiota dysbiosis in the gut of zebrafish[J]. Chemosphere, 2019,236:124334.
[43]	Zhao Y, Qiao R, Zhang S, et al. Metabolomic profiling reveals the intestinal toxicity of different length of microplastic fibers on zebrafish (Danio rerio)[J]. Journal of Hazardous Materials, 2021,403:123663.
[44]	Frost H, Bond T, Sizmur T, et al. A review of microplastic fibres:generation, transport, and vectors for metal(loid)s in terrestrial environments[J]. Environmental Science. Processes & Impacts, 2022,24(4):504-524.
[45]	Hu T, Shen M, Tang W. Wet wipes and disposable surgical masks are becoming new sources of fiber microplastic pollution during global COVID-19[J]. Environmental Science and Pollution Research International, 2022,29(1):284-292.
[46]	Choi C E, Zhang J, Liang Z. Towards realistic predictions of microplastic fiber transport in aquatic environments:Secondary motions[J]. Water Research, 2022,218:118476.
[47]	张瑞昌,李泽林,魏学锋,等.模拟环境老化对PE微塑料吸附Zn(II)的影响[J]. 中国环境科学, 2020,40(7):3135-3142. Zhang R C, Li Z L, Wei X F, et al. Effects of simulated environmental aging on the adsorption of Zn(II) onto PE microplastics[J]. China Environmental Science, 2020,40(7):3135-3142.
[48]	马思睿,李舒行,郭学涛.微塑料的老化特性、机制及其对污染物吸附影响的研究进展[J]. 中国环境科学, 2020,40(9):3992-4003. Ma S R, Li S X, Guo X T. A review on aging characteristics, mechanism of microplastics and their effects on the adsorption behaviors of pollutants[J]. China Environmental Science, 2020,40(9):3992-4003.