三种宽瓣亚属植物生境土壤真菌群落特征

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Abstract Paphiopedilum armeniacum, Paphiopedilum wenshanense and Paphiopedilum concolor as the research object, research habitat information, uncover the structural characteristics of soil fungal compositions and the associated environmental factors would provide the basis for efficient protection and cultivation. In this study, we investigated the habitat parameters and soil fungal community using samples collected from the natural habitats of the three species. The structural characteristics, diversity of fungal communities, fungal functional groups and the associated environmental factors were preliminarily analyzed by ITS high-throughput sequencing. A total of 1105 operational taxonomic units (OTUs) were obtained from 27 samples, 322 fungi were identified, belonging to 10 phyla, 29 classes, 70 orders, 151 families, 256 genera, respectively. There were 48 common OTUs in the habitat of three Subgen Brachypetalum species, 489 unique OTUs in P. concolor, 221 in P. wenshanense and 202 in P. armeniacum. There were different dominant groups at the taxonomic level of phylum, class, family and genus in the habitat of three Subgen Brachypetalum species. The fungal functional groups annotation found that the soil fungal functional groups in the habitat of three Subgen Brachypetalum species were dominated by saprophytic fungi and exogenous mycorrhizal fungi. The functional groups of habitats of P. concolor are more complex than other two species; Alpha diversity analysis results showed the congruence in the ACE index and Chao1index. The Simpson index and Shannon index of P. concolor were significantly higher than those of P. armeniacum, and were extremely significantly higher than those of P. wenshanense. Beta diversity analysis showed that there were significant differences in soil fungal community composition and structure among the habitat of three Subgen Brachypetalum species (P = 0.001), and the higher the classification level. Redundancy analysis found that latitude and longitude and vegetation types were the most important factors affecting the changes of soil communities of habitat of three Subgen Brachypetalum species, and all environmental factors accounted for 44.08% of the changes in fungal communities. Mantel test found the alpha diversity of soil fungi of habitat of three Subgen Brachypetalum species was significantly controlled by soil pH. Spearman correlation analysis confirmed that a variety of fungal dominant groups were significantly correlated with soil physical and chemical factors. Taken together, all these data suggest different composition of soil fungal communities, fungal functional groups and fungal diversity in the habitat of three Subgen Brachypetalum species. The composition and structure of soil fungal communities are mainly resulted from differences in environmental factors, and the distribution of dominant fungal communities are associated with changes in the soil physical and chemical properties.

Key words： Subgen Paphiopedilum high-throughput sequencing soil fungal communities habitat

Received: 31 October 2022

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	TIAN Li
	AN Ming-tai
	ZHANG Yang
	WU Mo-xu

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TIAN Li,AN Ming-tai,ZHANG Yang等. Characteristics of soil fungi community in wild populations of three Paphiopedilum Subgen Brachypetalum species[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1995-2006.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1995

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