抗生素对微藻的毒性作用和卤代烃释放的影响

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Abstract The toxicity of sulfamethazine (SMZ) and oxytetracycline (OTC) to Skeletonema costatum and the potential effects on the release of volatile halocarbons (VHCs) were investigated. The results showed that 10mg/L SMZ and OTC significantly inhibited the growth of Skeletonema costatum in the middle and later period of the culture process. The maximum inhibition rates of cell density, chlorophyll a concentration and F_v/F_m reached the maximum, which was 90.74%, 91.25%, 83.46%, 87.16%, 87.04% and 87.43%, respectively. The soluble protein content under antibiotic exposure was significantly lower than that in the control group. Catalase activity increased first and then decreased with the increase of antibiotic concentration and the prolongation of exposure time, indicating that long-term and high concentration exposure of the two antibiotics showed obvious toxic effects on microalgae, with the toxic effect of SMZ being higher than that of OTC. Antibiotic exposure induced an increase in intracellular reactive oxygen species (ROS) production. In addition, the addition of antibiotics can promote the release of VHCs in different degrees. It may be that the halogenation reaction of H₂O₂ catalyzed by haloperoxidase (HPO) produce more volatile halocarbons as antioxidants against oxidative stress to avoid the damage caused by excessive ROS to algal cells. These results indicate that the response of release of the three volatile halocarbons from microalgae to antibiotics was controlled by the biomass of algae and the catalytic mechanism of HPO enzyme.

Key words： antibiotics skeletonema costatum oxidative stress volatile halocarbons

Received: 22 August 2022

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X503.2

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	YAN Xue-feng
	YANG Bin
	YU Hong
	YANG Gui-peng
	HE Zhen

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YAN Xue-feng,YANG Bin,YU Hong等. Effects of antibiotics on toxicity and halocarbons release of microalgae[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1985-1994.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I4/1985

[1]	Carpenter L J, Yokouchi Y, Atlas E L. Introduction to special issue on natural halocarbons in the atmosphere[J]. Journal of Atmospheric Chemistry, 2017,74:141-143.
[2]	Quack B, Atlas E, Petrick G, et al. Oceanic bromoform sources for the tropical atmosphere[J]. Geophysical Research Letters, 2004,31(23):L23S05.
[3]	Liu Y N, Thornton D C O, Bianchi T S, et al. Dissolved organic matter composition drives the marine production of brominated very short-lived substances[J]. Environmental Science & Technology, 2015,49(6):3366-3374.
[4]	Qian Y A, Yang G A, Yue E A, et al. Methyl chloride produced during UV254 irradiation of saline water[J]. Journal of Hazardous Materials. 2020,384:121263.
[5]	Leedham E C, Hughes C, Keng F S L, et al. Emission of atmospherically significant halocarbons by naturally occurring and farmed tropical macroalgae[J]. Biogeosciences, 2013,10(6):3615-3633.
[6]	Abe M, Okuda Y, Hashimoto S. Effects of nutrient deficiency on the CH2I2, CH2ClI, and CH2BrI production in cultures of the temperate marine phytoplankton Bigelowiella natans [J]. Marine Chemistry, 2020,220:103765.
[7]	Hughes C, Franklin D J. Malin G. Iodomethane production by two important marine cyanobacteria:Prochlorococcus marinus (CCMP 2389) and Synechococcus sp. (CCMP 2370)[J]. Marine Chemistry, 2011,125:19-25.
[8]	Redeker K R, Chong J P J, Aguion A, et al. Microbial metabolism directly affects trace gases in (Sub) Polar snowpacks[J]. Journal of the Royal Society Interface, 2017,14(137):20170729.
[9]	Hill V L, Manley S L. Release of reactive bromine and iodine from diatoms and its possible role in halogen transfer in polar and tropical oceans[J]. Limnology and Oceanography, 2009,54(3):812-822.
[10]	Chuck A L, Turner S M, Liss P S. Oceanic distributions and air-sea fluxes of biogenic halocarbons in the open ocean[J]. Journal of Geophysical Research, 2005,110:C10022.
[11]	Lim Y K, Phang S M, Rahman N A, et al. Halocarbon emissions from marine phytoplankton and climate change[J]. International Journal of Environmental Science and Technology, 2017,14(6):1355-1370.
[12]	Lim Y K, Keng F S, Phang S M, et al. Effect of irradiance on the emission of short-lived halocarbons from three common tropical marine microalgae[J]. PeerJ, 2019,7:e6758.
[13]	Hughes C, Johnson M, von Glasow R. Climate-induced change in biogenic bromine emissions from the Antarctic marine biosphere[J]. Global Biogeochemical Cycles, 2012,26:GB3019.
[14]	Zhang R, Tang J, Li J, et al. Antibiotics in the offshore waters of the Bohai Sea and the Yellow Sea in China:Occurrence, distribution and ecological risks[J]. Environmental Pollution, 2013,174:71-77.
[15]	Xu W H, Yan W, Li X D, et al. Antibiotics in riverine runoff of the Pearl River Delta and Pearl River Estuary, China:Concentrations, mass loading and ecological risks[J]. Environmental Pollution, 2013,182:402-407.
[16]	Siedlewicz G, Zak A, Sharma L, et al. Effects of oxytetracycline on growth and chlorophyll a fluorescence in green algae (Chlorella vulgaris), diatom (Phaeodactylum tricornutum) and cyanobacteria (Microcystis aeruginosa and Nodularia spumigena)[J]. Oceanologia, 2020,62(2):214-225.
[17]	Xiong J Q,Kim S J, Kurade M B, et al. Combined effects of sulfamethazine and sulfamethoxazole on a freshwater microalga Scenedesmus obliquus:toxicity, biodegradation, and metabolic fate[J]. Journal of Hazardous Materials, 2019,370:138-146.
[18]	Guo J H, Peng J L, Lei Y, et al. Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris[J]. Aquatic Toxicology, 2020, 219:105376.
[19]	Abrahamsson K, Choo K S, M Pedersén, et al. Effects of temperature on the production of hydrogen peroxide and volatile halocarbons by brackish-water algae[J]. Phytochemistry, 2003,64(3):725-734.
[20]	Hughes C, Sun S. Light and brominating activity in two species of marine diatom[J]. Marine Chemistry, 2016,181:1-9.
[21]	Abe M, Nagai T, Kurihara M, et al. Effect of temperature on the methyl chloride production rate in a marine phytoplankton, Phaeodactylum tricornutum [J]. Journal of Atmospheric Chemistry, 2017,74(2):157-169.
[22]	Nguvava M, Kuyper B, Bucciarelli E, et al. Nutrient limitation in two marine diatoms inhibits release of bromoform[J]. African Journal of Marine Science, 2016,38(4):581-588.
[23]	Han Y, He Z, Yang G P. Distributions of volatile halocarbons and impacts of ocean acidification on their production in coastal waters of China[J]. Science of the Total Environment, 2021,752:141756.
[24]	Lyu J, Yang L S, Zhang L, et al. Antibiotics in soil and water in Chinaea systematic review and source analysis[J]. Environmental Pollution, 2020,266:115147.
[25]	Wang X T, Lin Y F, Zheng Y, et al. Antibiotics in mariculture systems:A review of occurrence, environmental behavior, and ecological effects[J]. Environmental Pollution, 2022,293:118541.
[26]	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976,72:248-254.
[27]	Nie X P, Liu B Y, Yu H J, et al. Toxic effects of erythromycin, ciprofloxacin and sulfamethoxazole exposure to the antioxidant system in Pseudokirchneriella subcapitata[J]. Environmental Pollution, 2013,172:13-32.
[28]	Yuan D, Yang G P, He Z. Spatio-temporal distributions of chlorofluorocarbons and methyl iodide in the Changjiang (Yangtze River) estuary and its adjacent marine area[J]. Marine Pollution Bulletin, 2016,103(1):247-259.
[29]	Zhou Z, Zhang Z, Feng L, et al. Adverse effects of levofloxacin and oxytetracycline on aquatic microbial communities[J]. Science of the Total Environment, 2020,734:139499.
[30]	Wang Y, Gong X Y, Huang D Y, et al. Increasing oxytetracycline and enrofloxacin concentrations on the algal growth and sewage purification performance of an algal-bacterial consortia system[J]. Chemosphere, 2022,286:131917.
[31]	Xiong J Q, Kurade M B, Jeon B H. Ecotoxicological effects of enrofloxacin and its removal by monoculture of microalgal species and their consortium[J]. Environmental Pollution, 2017,226:486-493.
[32]	Wang M X, Zhang Y X, Guo P Y. Effect of florfenicol and thiamphenicol exposure on the photosynthesis and antioxidant system of Microcystis flos-aquae[J]. Aquatic Toxicology, 2017,186:67-76.
[33]	Xiong J Q, Kurade M B, Abou-Shanab R, et al. Biodegradation of carbamazepine using freshwater microalgae Chlamydomonas mexicana and Scenedesmus obliquus and the determination of its metabolic fate[J]. Bioresource Technology, 2016,205:183-190.
[34]	Nie X P, Wang X, Chen J, et al. Response of the freshwater alga Chlorella vulgaris to trichloroisocyanuric acid and ciprofloxacin[J]. Environmental Toxicology & Chemistry, 2008,27(1):168-173.
[35]	Oh M H, Kang Y H, Lee C H, et al. Effects of six antibiotics on the activity of the photosynthetic apparatus and ammonium uptake of thallus of Porphyra yezoensis[J]. Algae, 2005,20(2):121-125.
[36]	Chen S C, Chen M D, Wang Z, et al. Toxicological effects of chlorpyrifos on growth, enzyme activity and chlorophyll a synthesis of freshwater microalgae[J]. Environmental Toxicology & Pharmacology, 2016,45:179-186.
[37]	Wang Y J, Li Y W, Luo X Y, et al. Effects of yttrium and phosphorus on growth and physiological characteristics of Microcystis aeruginosa[J]. Journal of Rare Earths, 2018,36(7):781-788.
[38]	Xu D M, Xiao Y P, Pan H, et al. Toxic effects of tetracycline and its degradation products on freshwater green algae[J]. Ecotoxicology and Environmental Safety, 2019,174:43-47.
[39]	Baruah P, Chaurasia N. Ecotoxicological effects of alpha-cypermethrin on freshwater alga Chlorella sp.:Growth inhibition and oxidative stress studies[J]. Environmental Toxicology and Pharmacology, 2020,76:103347.
[40]	Cai H L, Liang J Y, Ning X A, et al. Algal toxicity induced by effluents from textile-dyeing wastewater treatment plants[J]. Journal of Environmental Sciences, 2020,91:199-208.
[41]	Tsiaka P, Tsarpali V, Ntaikou I, et al. Carbamazepine-mediated pro-oxidant effects on the unicellular marine algal species Dunaliella tertiolecta and the hemocytes of mussel Mytilus galloprovincialis[J]. Ecotoxicology, 2013,22(8):1208-1220.
[42]	Lang X P, Ni J, He Z. Effects of polystyrene microplastic on the growth and volatile halocarbons release of microalgae Phaeodactylum tricornutum[J]. Marine Pollution Bulletin, 2022,174:113197.
[43]	Lim Y K, Keng F S, Phang S M, et al. Effect of irradiance on the emission of short-lived halocarbons from three common tropical marine microalgae[J]. PeerJ, 2019,7:e6758.
[44]	Wever R, Van D. The role of vanadium haloperoxidases in the formation of volatile brominated compounds and their impact on the environment[J]. Dalton Transactions, 2013,42(33):11778-11786.
[45]	Moore R M, Webb M, Tokarczyk R, et al. Bromoperoxidase and iodoperoxidase enzymes and production of halogenated methanes in marine diatom cultures[J]. Journal of Geophysical Research Oceans, 1996,101(C9):20899-20908.
[46]	郎小平,何真,陈岩,等.微塑料对微藻生长及其释放三卤代甲烷的影响[J]. 中国环境科学, 2021,41(12):5857-5865. Lang X P, He Z, Chen Y, et al. The influence of microplastic on the growth of microalage and their release of trihalomethane[J]. China Environmental Science, 2021,41(12):5857-5865.
[47]	Manley S L, Barbero P E. Physiological constraints on bromoform (CHBr3) production by Ulva lactuca (Chlorophyta)[J]. Limnology and Oceanography, 2001,46(6):1392-1399.
[48]	Bai X, Acharya K. Removal of trimethoprim, sulfamethoxazole, and triclosan by the green alga Nannochloris sp.[J]. Journal of Hazardous Materials, 2016,315:70-75.
[49]	Marshall J A, Hovenden M, Oda T, et al. Photosynthesis does influence superoxide production in the ichthyotoxic alga Chattonella marina (Raphidophyceae)[J]. Journal of Plankton Research, 2002,24(11):1231-1236.