西北内陆河抗生素抗性基因赋存特征及其影响因素

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1873 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The distribution characteristics of antibiotic resistance genes (ARGs) and bacterial community structure in surface water of Y River Basin were analyzed based on metagenomics. The relationship between ARGs and environmental factors and bacterial communities was investigated. The results showed that 21 types of ARGs and 1295 subtypes of ARGs, 6resistance mechanisms were detected in the surface water of Y River basin, among which multidrug was the main type of ARGs, macB was the dominant subtype of ARGs, and antibiotic effluents were the main resistance mechanisms.Bacteria were detected at 160 phylum levels and 4149 genus levels, respectively, among which Proteobacteria is the dominant bacteria phylum, Liminohabitans and others are the dominant bacteria genera. The correlation analysis showed that TOC, DOC and COD_Mn were significantly correlated with various ARGs subtypes(P<0.05); Some genera, such as Flavobacterium, Polaromonas, and Nocardioides, were significantly associated with multiple ARGs (P<0.05) and involved multiple type and resistance mechanisms of ARGs .This study aims to provide data support for ARGs distribution characteristics of typical inland river environment in northwest arid region.

Key words： antibiotic resistance gene bacterial community environmental factors metagenomics inland northwest rivers

Received: 09 November 2022

PACS:

X522

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XING Yue
	ZHANG Wen
	CHENG Yan
	CHENG Gang
	HU Hai-yang

Cite this article:

XING Yue,ZHANG Wen,CHENG Yan等. Distribution characteristics and influencing factors of antibiotic resistance genes in inland rivers of Northwest China[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 3077-3086.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I6/3077

[1]	Qiao M, Ying G G, Singer A C, et al. Review of antibiotic resistance in China and its environment[J]. Environment International, 2018, 110(1):160-172.
[2]	Pruden A, Pei R, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants:studies in northern colorado[J]. Environmental Science & Technology, 2006,40(23):7445-7450.
[3]	Gillings M R. Lateral gene transfer, bacterial genome evolution, and the Anthropocene[J]. Annals of the New York Academy of Sciences, 2017,1389:20-36.
[4]	Han Z, Zhang Y, An W, et al. Antibiotic resistomes in drinking water sources across a large geographical scale:multiple drivers and co-occurrence with opportunistic bacterial pathogens[J]. Water Research, 2020,183:116088.
[5]	罗晓,袁立霞,张文丽,等.制药废水厂抗性基因和微生物群落相关性研究[J]. 中国环境科学, 2019,39(2):831-838. Luo X, Yuan L X, Zhang W L, et al. Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants[J]. China Environmental Science, 2019, 39(2):831-838.
[6]	Murray C, Ikuta K S, Sharara F, et al. Global burden of bacterial antimicrobial resistance in 2019:a systematic analysis[J]. The Lancet, 2022,399(10325):629-655.
[7]	Dang C Y, Xia Y, Zheng M S, et al. Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir[J]. Environment International, 2020,136:105449.
[8]	Wan K, Lin W, Zhu S, et al. Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water:a review and meta-analysis[J]. Frontiers of Environmental Science & Engineering, 2019,14(1):10-23.
[9]	Bkb A, Hjc A. Metagenomic study focusing on antibiotic resistance genes from the sediments of River Yamuna[J]. Gene, 2020,758:144951.
[10]	Chen H, Jing L, Yao Z, et al. Prevalence, source and risk of antibiotic resistance genes in the sediments of Lake Tai (China) deciphered by metagenomic assembly:a comparison with other global lakes[J]. Environment International, 2019,127:267-275.
[11]	Zhang H K, Wang Y B, Liu P Y, et al. Unveiling the occurrence, hosts and mobility potential of antibiotic resistance genes in the deep ocean[J]. Science of the Total Environment, 2021,816:151539.
[12]	Lu J, Zhang Y X, Wu J, et al. Occurrence and spatial distribution of antibiotic resistance genes in the Bohai Sea and Yellow Sea areas[J]. China Environmental Pollution, 2019,252:450-460.
[13]	Zhang Y X, Wang J H, Lu J, et al. Antibiotic resistance genes might serve as new indicators for wastewater contamination of coastal waters:spatial distribution and source apportionment of antibiotic resistance genes in a coastal bay[J]. Ecological Indicators, 2020,114:106299.
[14]	李十盛,高会,赵富强,等.水产养殖环境中抗生素抗性基因的研究进展[J]. 中国环境科学, 2021,41(11):5314-5325. Li S S, Gao H, Zhao F Q, et al. Research progress on the occurrence and influencing factors of antibiotic resistance genes in aquaculture environment[J] China Environmental Science, 2021,41(11):5314-5325.
[15]	Port J A, Cullen A C, Wallace J C, et al. Metagenomic frameworks for monitoring antibiotic resistance in aquatic environments[J]. Environmental Health Perspect, 2014,122(3):222-228.
[16]	Chen S, Zhou Y, Chen Y, et al. Fastp:an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018,34(17):884-890.
[17]	Li D, Liu C M, Luo R, et al. Megahit:an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph[J]. Bioinformatics, 2015,31(10):1674-1676.
[18]	Noguchi H, Park J, Takagi T, et al. Metagene:prokaryotic gene finding from environmental genome shotgun sequences[J]. Nucleic Acids Res, 2006,34(19):5623-5630.
[19]	Fu L, Niu B, Zhu Z, et al. CD-hit:accelerated for clustering the next-generation sequencing data[J]. Bioinformatics, 2012,28(23):3150-3152.
[20]	Li R, Li Y, Kristiansen K, et al. Soap:short oligonucleotide alignment program[J]. Bioinformatics, 2008,24(5):713-714.
[21]	Lawson C E, Wu S, Bhattacharjee A S, et al. Metabolic network analysis reveals microbial community interactions in anammox granules[J]. Nature Communications, 2017,8:15426.
[22]	Buchfink B, Xie C, Huson D H, et al. Fast and sensitive protein alignment using diamond[J]. Nat Methods, 2015,12(1):59-60.
[23]	Wang J H, Lu J, Zang Y X, et al. Metagenomic analysis of antibiotic resistance genes in coastal industrial mariculture systems[J]. Bioresource Technology, 2018,253:235-243.
[24]	周婷,李鑫,王晓龙,等.新疆玛纳斯河流域抗生素抗性基因的污染分析[J]. 石河子大学学报(自然科学版), 2014,32(6):755-759. Zhou T, Li X, Wang X L, et al. Pollution analysis of antibiotic resistance genes of Manas River Basin, Xinjiang[J]. Journal of Shihezi University (Natural Science), 2014,32(6):755-759.
[25]	张泽辉,宋雪娇,黄程程,等.细菌的获得性耐药机制研究进展[J]. 动物医学进展, 2017,38(1):74-77. Zhang Z H, Song X J, Huang C C, et al. Progress on antimicrobial resistance by change of target protein in bacteria[J]. Progress in Veterinary Medicine, 2017,38(1):74-77.
[26]	丰新倩,韩阿祥,黄杰鹏,等.宏基因组分析城市休闲水域九山湖的微生物群落结构和抗性基因(英文)[J]. 微生物学通报, 2020,47(10):3102-3113. Feng X Q, Han A X, Huang J P, et al. Metagenomic analysis of the microbial community and antibiotic resistance genes in an urban recreational lake, Jiushan Lake[J]. Microbiology China, 2020,47(10):3102-3113.
[27]	Chen H, Chen R, Jing L, et al. A metagenomic analysis framework for characterization of antibiotic resistomes in river environment:Application to an urban river in Beijing[J]. Environmental Pollution, 2019,245:398-407.
[28]	陈小燕,潘珏.细菌耐多药外排泵的研究进展[J]. 微生物与感染, 2016,11(3):183-187. Chen X Y, Pan Y. Research progress on bacterial multidrug efflux pump and biofilm formation[J]. Journal of Microbes and Infections, 2016,11(3):183-187.
[29]	姚明晓,苑广盈,闻新棉.主动外排系统介导细菌多重耐药的研究进展[J]. 现代预防医学, 2014,41(1):118-121. Yao M X, Yuan G Y. Wen X M, et al. The research progress on active efflux system mediated multidrug resistance in bacteria[J]. Modern Preventive Medicine, 2014,41(1):118-121.
[30]	Suguru Y, Eiji N, Ryosuke N, et al. The crystal structure of multidrug-resistance regulator Ramr with multiple drugs[J]. Nature Communtion, 2013,4:2078.
[31]	刘灿均,门宝辉,牛晓赟,等.基于集对分析的滦河干流承德段水质评价[J]. 水生态学杂志, 2022,43(5):22-32. Liu C J, Men B H, Niu X B, et al. Water quality evaluation of the Chengde section of Luanhe River based on set pair analysis[J]. Journal of Hydroecology, 2022,43(5):22-32.
[32]	高榆,冯小飞,闻静,等.2012~2017年中国丙型肝炎与HIV/AIDS报告病例的时空分布及相关性分析[J]. 中华流行病学杂志, 2019, 40(2):155-159. Gao Y, Feng X F, Wen J, et al. Spatial and temporal distribution and correlation analysis of reported cases of hepatitis and HIV/AIDS in China from 2012 to 2017[J]. Chinese Journal of Epidemiology, 2019, 40(2):155-159.
[33]	Jian H G, Jie L, Hui C, et al. Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements[J]. Water research, 2018,123:468-478.
[34]	Gao H, Zhao F Q, Li R J, et al. Occurrence and distribution of antibiotics and antibiotic resistance genes in water of Liaohe River Basin, China[J]. Journal of Environmental Chemical Engineering, 2022,10(5):108297.
[35]	Jia J, Guan Y J, Cheng M Q, et al. Occurrence and distribution of antibiotics and antibiotic resistance genes in Ba River, China[J]. Science of the Total Environment, 2018,642:1136-1144.
[36]	彭韵,李蕾,伍迪,等.微生物群落对氨胁迫响应的宏基因组学研究[J]. 中国环境科学, 2022,42(2):777-786. Peng Y, Li L, Wu D, et al. Metagenomic analysis on the responses of microbial community to ammonia stress[J] China Environmental Science, 2022,42(2):777-786.
[37]	Larsen P, Nielsen J L, Otzenj D, et al. Amyloid-like adhesins produced by floc-forming and filamentous bacteria in activated sludge[J]. Applied and Environmental Microbiology, 2008,74(5):1517-1526.
[38]	冯希.海洋疣微菌的分离、鉴定及疣微菌门分类体系整理[D]. 济南:山东大学, 2021. Feng X. Isolation, polyphasic taxonomy of marine strains of verrucomicrobia and updatioan the classificatioan system of verrucomicrobia[D]. Jinan:Shangdong University, 2021.
[39]	刘欢.黄杆菌属等三株新种的分离和多相分类研究[D]. 南京:南京农业大学, 2008. LIU H. Studies on Isolation and polyphasic taxonomy of Flavobacterium and other three new species[D]. Nanjing:Nanjing Agricultural University, 2008.
[40]	徐秋鸿,刘曙光,娄厦,等.长江口近岸地区抗生素抗性基因与微生物群落分布特征[J]. 环境科学, 2022,1-16.DOI:10.13227/j.hjkx. 202203160. Xu Q H, Liu S G, Lou X, et al. Distributions of antibiotic resistance genes and microbial communities in the nearshore area of the Yangtze River estuary[J]. Environmental Science, 2022,1-16.DOI:10.13227/j.hjkx.202203160.
[41]	Gou X P, Yang Y, Lu D P, et al. Biofilms as a sink for antibiotic resistance genes (ARGs) in the Yangtze Estuary[J]. Water Research, 2018,129:277-286.
[42]	秦荣,喻庆国,刘振亚,等.人类活动影响下的高原湿地四环素类抗生素抗性基因赋存与微生物群落共现性[J]. 环境科学, 2022, 1-16.DOI:10.13227/j.hjkx.202203109. Qin R, Yu Q G, Liu Z Y, et al. Co-occurrence of Tetracycline antibiotic resistance genes and microbial communities in plateau wetlands under the influence of human activities[J]. Environmental Science, 2022, 1-16.DOI:10.13227/j.hjkx.202203109.