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| A review on application of stable isotope probing combined with metagenomics in studying the biotransformation and degradation of environmental pollutants |
| ZHANG Huan-jun, ZHOU Jing-ya, LI Yi |
| Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China |
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Abstract The application of stable isotope probing (SIP) combined with metagenomics in the research of environmental pollutants biotransformation and degradation has gradually become a trend. This combined technology provides a culture-independent method to identify functional microbial community with specific metabolic activities and to reveal key metabolic pathways in complex environment, so it has significant advantages in studying in situ biotransformation and degradation of environmental pollutants. This review introduces the application of this combined technology in studying the biotransformation and degradation of hydrocarbons and their derivatives, pesticides, heavy metals and emerging contaminants in different environments, including identification of functional microbial community and mechanism of pollutants biotransformation and degradation. Finally, future prospect is presented based on the current application status of this combined technology. The combined technology associated with multiple approaches to in situ characterization will provide more accurate and comprehensive information for studying the mechanism of biotransformation and degradation of pollutants driven by microorganisms.
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Received: 07 November 2022
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