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| Effect of inoculum and cultivation mode on the performances of denitrifying biocathodes |
| ZHAO Ting, FENG Yong, XIE Bei-zhen, LIU Hong |
| Institute of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China |
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Abstract This study investigated the effects of inoculum and cultivation mode on denitrifying bioelectrochemmical systems (BESs). Firstly, several inocula, including freshwater sediment, activated sludge and heterotrophic denitrifiers, were used to construct directly start-up BESs (DS-BESs). All the DS-BESs could reduce nitrate with electrodes as electron donors, but the nitrate-N removal rates were only 11.9~25.2mg/(m2·d). Among them, the DS-BESs inoculated with freshwater sediment showed relatively high nitrate-N removal performances. Then, the BESs obtained by polarity reversal (PR-BESs) were constructed with freshwater sediment and anaerobic activated sludge as inocula. The nitrate-N removal rates of the PR-BESs achieved 227.4mg/(m2·d)and 160.6mg/(m2·d), respectively, which were much higher than those of the DS-BESs. 16S rDNA sequencing analysis revealed that inocula had no significant effect on the dominant microbial species growing on BES electrode surfaces. Afipia and Aquamicrobium were enriched and became dominant genera in both the DS-BESs and PR-BESs. Moreover, biomass analysis showed that the DNA contents on the electrode surface of PR-BESs were much higher than those on the electrode of DS-BESs, indicating that the enhanced nitrate removal performances by the PR mode can be mainly attributed to the increased biomass on BES electrode surfaces.
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Received: 30 November 2022
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