脱水时长对间歇性河流生物膜反硝化功能的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1082 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract This study took the biofilms cultivated in artificially simulated rivers as the test subjects, and explored the dynamic response of biofilms denitrification to rewetting process after short-term drought (25days) and long-term drought (90days). The results showed that the water content and macro-microscopic morphology of biofilm changed significantly during both drought and rewetting, and the water content dropped sharply in the 3d to 7d, from (74.96±3.57)% to (2.38±0.37)%. The denitrification rate of both short and long term drought groups showed a continuous increase after rewetting. The short-term drought group showed a significant increase (P<0.05), from (3.26±0.12) to (5.35±1.18)μmol N₂-¹⁵N/(L·h), while the long-term drought group was concentrated in the late rewetting period, from (2.14±0.19) to (4.15±0.89)μmol N₂-¹⁵N/(L·h) (P <0.05). Biofilms that experienced short-term drought were able to recover to the initial denitrification rate level through the rewetting process, while those that experienced long-term drought had difficulty achieving it (P<0.05), indicating that long-term drought had an irreversible effect on the denitrification rate of biofilm. The activities of denitrification functional enzymes also showed different changes during each rehydration stage. Short-term drought significantly increased the nitric oxide reductase activity at the end of the reaction pathway (P<0.05). While the nitrite reductase and nitrite oxide reductase, which directly affect the denitrification rate, also had difficulty in recovering to their initial levels after experiencing long-term drought (P<0.05). The above results show that prolonged drought significantly alters the biogeochemical cycle driven by river biofilms (denitrification function).

Key words： biofilm flow intermittency denitrification functional enzyme

Received: 15 January 2023

PACS:

X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	MIAO Ling-zhan
	LI Wei-yu

Cite this article:

MIAO Ling-zhan,LI Wei-yu. Effects of drought duration on the denitrification of biofilms in intermittent river[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 3918-3925.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I8/3918

[1]	Colls M, Timoner X, Font C, et al. Effects of duration, frequency, and severity of the non-flow period on stream biofilm metabolism[J]. Ecosystems, 2019,22(6):1393-1405.
[2]	Tonkin J D, Merritt D M, Olden J D, et al. Flow regime alteration degrades ecological networks in riparian ecosystems[J]. Nature Ecology & Evolution, 2018,2(1):86-93.
[3]	刘开朗,王加启,卜登攀,等.环境微生物群落结构与功能多样性研究方法[J].生态学报, 2015,30(4):1074-1080. Liu K L, Wang J Q, Bu D P, et al. Current progress in approaches to the study of structure and function diversities of environmental microbial communities[J]. Acta Ecologica Sinica, 2015,30(4):1074-1080.
[4]	陈慧清,李晓晨,于学峰,等.土壤生态系统微生物多样性技术研究进展[J].地球与环境, 2018,46(2):204-209. Chen H Q, Li X C, Yu X F, et al. A Review on Technique Progresses of Microbial Diversity in Soil Ecosystem[J]. Earth and Environment 2018,46(2):204-209.
[5]	穆光熠.河流水体CDOM光学特性及其对生态环境要素的响应[D].吉林:东北师范大学, 2019. Mu G Y. CDOM optical properties of river water body and its response to ecological environment elements[D]. Jilin:Northeast Normal University, 2019.
[6]	蒲高忠.水生态系统中凋落物降解对环境压力的响应[D].南京:南京大学, 2014. Pu G Z. Response of litter degradation to environmental stress in water ecosystems[D]. Nanjing:Nanjing University, 2014.
[7]	Battin T, Besemer K, Bengtsson M, et al. The ecology and biogeochemistry of stream biofilms[J]. Nature Reviews Microbiology, 2016,14(4):251-263.
[8]	Lv T, Carvalho P N, Zhang L, et al. Functionality of microbial communities in constructed wetlands used for pesticide remediation:Influence of system design and sampling strategy[J]. Water Research, 2017,110(MAR.1):241-251.
[9]	刘洋荧,王尚,厉舒祯,等.基于功能基因的微生物碳循环分子生态学研究进展[J].微生物学通报, 2017,44(7):1676-1689. Liu Y Y, Wang S, Li S Z, et al. Advances in molecular ecology on microbial functional genes of carbon cycle[J]. Microbiology China, 2017,44(7):1676-1689.
[10]	贺云龙,齐玉春,彭琴,等.外源碳输入对陆地生态系统碳循环关键过程的影响及其微生物学驱动机制[J].生态学报, 2017,37(2):358-366. He Y L, Qi Y C, Peng Q, et al. Effects of external carbon on the key processes of carbon cycle in a terrestrial ecosystem and its microbial driving mechanism[J]. Acta Ecologica Sinica, 2017,37(2):358-366.
[11]	Smeti E, Schiller D V, Karaouzas I, et al. Multiple stressor effects on biodiversity and ecosystem functioning in a Mediterranean temporary river[J]. Science of the Total Environment, 2019,647:1179-1187.
[12]	黄帅辰,左剑恶,陈磊,等.排水管道沉积物微生物群落及环境因子分析[J].中国环境科学, 2020,40(12):5369-5374. Huang S C, Zuo J E, Chen L, et al. Analysis of microbial communities and environmental factors in sewer sediments[J]. China Environmental Science, 2020,40(12):5369-5374.
[13]	李超然,苗令占,侯俊.间歇性河流中生物膜对干湿胁迫的动态响应[J].中国环境科学, 2021,41(11):5245-5253. Li C R, Miao L Z, Hou J. Dynamic response of biofilm to dry and wet stress in intermittent rivers[J]. China Environmental Science, 2021,41(11):5245-5253.
[14]	Timoner X, Borrego C M, Acua V, et al. The dynamics of biofilm bacterial communities is driven by flow wax and wane in a temporary stream[J]. Limnology and Oceanography, 2015,59(6):2057-2067.
[15]	Sun S, Liu J, Zhang M, et al. Simultaneous improving nitrogen removal and decreasing greenhouse gas emission with biofilm carriers addition in ecological floating bed[J]. Bioresource Technology, 2019,292:121944.
[16]	Austin B J, Strauss E A. Nitrification and denitrification response to varying periods of desiccation and inundation in a western Kansas stream[J]. Hydrobiologia, 2011,658(1):183-195.
[17]	Gómez R, Arce M I, Sánchez J J, et al. The effects of drying on sediment nitrogen content in a Mediterranean intermittent stream:a microcosms study[J]. Hydrobiologia, 2012,679(1):43-59.
[18]	Sabater S, Timoner X, Borrego C, et al. Stream biofilm responses to flow intermittency:from cells to ecosystems[J]. Frontiers in Environmental Science, 2016,4.
[19]	Miao L, Yu Y, Adyel T M, et al. Distinct microbial metabolic activities of biofilms colonizing microplastics in three freshwater ecosystems[J]. Journal of Hazardous Materials, 2020,403:123577.
[20]	Wu Y, Xia L, Yu Z, et al. In situ bioremediation of surface waters by periphytons[J]. Bioresource Technology, 2014,151:367-372.
[21]	Zhao Y, Xiong X, Wu C, et al. Influence of light and temperature on the development and denitrification potential of periphytic biofilms[J]. Science of the Total Environment, 2018,613-614:1430-1437.
[22]	黄晚华,隋月,杨晓光,等.基于连续无有效降水日数指标的中国南方作物干旱时空特征[J].农业工程学报, 2014,30(4):125-135. Huang W H, Sui Y, Yang X G, et al. Spatio-temporal characteristics of crop drought in southern China based on drought index of continuous days without available precipitation[J]. Transactions of the Chinese Society of Agricultural Engineering, 2014,30(4):125-135.
[23]	Gionchetta G, Romaní A M, Oliva F, et al. Distinct responses from bacterial, archaeal and fungal streambed communities to severe hydrological disturbances[J]. Scientific Reports, 2019,9:13506.
[24]	Colls M, Timoner X, Font C, et al. Effects of duration, frequency, and severity of the non-flow period on stream biofilm metabolism[J]. Ecosystems, 2019,22(6):1393-1405
[25]	Stanley E H, Fisher S G, Jones J. Effects of water loss on primary production:a landscape-scale model[J]. Aquatic Sciences, 2004,66(1):130-138.
[26]	Sigee D C. Freshwater microbiology:biodiversity and dynamic interactions of microorganisms in the aquatic environment[J]. Translation & Interpreting, 2005,3(2):401-441.
[27]	Mcclain M E, Boyer E W, Dent C L, et al. Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems[J]. Ecosystems (New York), 2003,6(4):301-312.
[28]	Liu S, Wang C, Hou J, et al. Effects of Ag NPs on denitrification in suspended sediments via inhibiting microbial electron behaviors[J]. Water Research, 2020,171:115436.
[29]	王淑莹,孙洪伟,杨庆,等.传统生物脱氮反硝化过程的生化机理及动力学[J].应用与环境生物学报, 2008,14(5):732-736. Wang S Y, Sun H W, Yang Q, et al. Biochemical reaction mechanism and kinetics of denitrification[J]. Chinese Journal of Applied and Environmental Biology 2008,14(5):732-736.
[30]	Sanja Zlatanović, Fabian J, Premke K, et al. Shading and sediment structure effects on stream metabolism resistance and resilience to infrequent droughts[J]. Science of the Total Environment, 2017,621(APR.15):1233-1242.
[31]	Timoner X, Acuña V, Schiller D V, et al. Functional responses of stream biofilms to flow cessation, desiccation and rewetting[J]. Freshwater Biology, 2012,57(8):1565-1578.
[32]	Timoner X, Acuña V, Frampton L, et al. Biofilm functional responses to the rehydration of a dry intermittent stream[J]. Hydrobiologia, 2014,727(1):185-195.
[33]	Unger S, Máguas C, Pereira J S, et al. The influence of precipitation pulses on soil respiration-Assessing the"Birch effect" by stable carbon isotopes[J]. Soil Biology & Biochemistry, 2010,42(10):1800-1810.
[34]	Su X, Chen Y, Wang Y, et al. Impacts of chlorothalonil on denitrification and N2O emission in riparian sediments:microbial metabolism mechanism[J]. Water Research, 2018,148(JAN.1):188-197.