有机废水中硫酸盐和氨的厌氧转化机制研究

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Abstract By gradually increasing the influent n(NH₄⁺-N)/n(SO₄^2-) ratio, sulfate reduction-ammonia oxidation (SRAO) was achieved in an up-flow anaerobic sludge blanket (UASB) reator under anaerobic organic conditions (ORP=(-334.3±18.2) mV, DO=(0.10±0.5) mg/L). The effects of different COD on the removel performance of nitrogen and sulfur were studied. The transformation mechanism was discussed by analyzing the SRAO products, the operation results of different stages and the variation of microbal flora. The results showed that the SRAO was that SO₄^2- oxidized NH₄⁺-N to NO₂^--N and generated S to accumulate in the sludge phase. The intermediate product NO₂^--N was reduced by autotrophic/heterotrophic denitrifying bacteria, and part of SO₄^2- was reduced to S^2- by sulfate reducing bacteria (SRB). Synergistic metabolism among microorganisms achieved simultaneous removal of nitrogen and sulfur in the system. Increasing the influent N/S ratio can make more NH₄⁺-N converted to NO₂^--N to be utilized by denitrifying bacteria, but when the N/S ratio was increased to 4, the sulfur autotrophic denitrification strongly affected the removal of SO₄^2-, and the abundance of Thiobacillus in the reactor increased by 3.36% at this stage. The simultaneous removal of NH₄⁺-N and SO₄^2- did not occur under inorganic conditions without organic matter. Increasing the influent COD concentration to 150mg/L promoted the coupling of SRAO and denitrification, and improved the nitrogen and sulfur removal performance of the system. The total abundance of SRB (Desulfococcus, Desulfatiglans) and various denitrifying bacteria (Methyloversatilis, Longilinea, Simplicispira, Bdellovibrio, Azospira, etc.) in the reactor increased by 1.584% and 6.081%, respectively. When COD=250mg/L, the enhanced sulfated reduction reaction produced more S^2-, which inhibited the SRAO activity and affected the removal of NH₄⁺-N in the system.

Key words： nitrogen and sulfur removal sulfate reduction denitrification sulfur autotrophic denitrification

Received: 17 January 2023

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X703

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	REN Hong-li
	YUAN Lin-jiang
	CHEN Xi

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REN Hong-li,YUAN Lin-jiang,CHEN Xi. Study on anaerobic conversion mechanism of sulfate and ammonia in organic wastewater[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 3926-3934.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I8/3926

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