LCFAs降解动力学特性及对厌氧消化各阶段的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1314 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract Long chain fatty acids (LCFAs) affect the stability of anaerobic digestion system. The methanogenic properties of 3typical LCFAs (palmitate C_16:0, stearate C_18:0, oleate C_18:1) at different concentrations and their effects on anaerobic digestion were studied. The methanogenic results showed that the complete degradation of oleate was significantly slower (26d), but the specific methanogenic activity (SMA) was higher, which was 1.2 and 2.7 times that of palmitate and stearate. The lag time (t₀) and the maximum methane production rate (R_max) of the oleate group increased linearly with the rising of the concentration. Thus it can be seen that the influence of saturation degree on the degradation kinetics of LCFAs was greater than the carbon chain length. Microbial community analysis showed that the abundance of Clostridium_sensu_stricto and Petrimonas in the oleate group was higher (10.38% and 10.03%). This may be due to the polyhydroxyl LCFAs produced by saturation before β-oxidation, which degrade more slowly. The addition of oleate also enriched Methanosarcina, Methanosobacterium and Methanomassiliicoccus, strengthening the hydrogenotrophic methanogenesis pathway. In addition, the 3LCFAs had inhibitory effects on each stage of anaerobic digestion. The inhibitory degree was the strongest on methanogenesis (16.51%, 40.49%, 63.77%, respectively), and was affected by both the carbon chain length and saturation degree.

Key words： long chain fatty acids (LCFAs) anaerobic degradation anaerobic digestion kinetics

Received: 21 December 2022

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XI Yu
	LU Bin
	CHEN Hao
	ZHANG Meng-na
	LI Qian

Cite this article:

XI Yu,LU Bin,CHEN Hao等. Degradation kinetics of LCFAs and effects on anaerobic digestion[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 4080-4088.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I8/4080

[1]	Diamantis V, Eftaxias A, Stamatelatou K, et al. Bioenergy in the era of circular economy:Anaerobic digestion technological solutions to produce biogas from lipid-rich wastes[J]. Renewable Energy, 2021, 168:438-447.
[2]	李亚林,刘蕾,关明玥,等."双碳"目标下餐厨垃圾资源化技术及其碳排放分析[J].河南工程学院学报(自然科学版), 2022,34(2):42-47. Li Y L, Liu L, Guan M Y, et al. Technology of food wastes recycling and its carbon emission analysis under the targets of carbon peak and carbon neutrality[J]. Journal of Henan University of Engineering (Natural Science Edition), 2022,34(2):42-47.
[3]	Neves L, Oliveira R, Alves M M. Fate of LCFA in the co-digestion of cow manure, food waste and discontinuous addition of oil[J]. Water research, 2009,43(20):5142-5150.
[4]	Elsamadony M, Mostafa A, Fujii M, et al. Advances towards understanding long chain fatty acids-induced inhibition and overcoming strategies for efficient anaerobic digestion process[J]. Water Research, 2021,190:116732.
[5]	Wei T, Fang Q, Luo J, et al. Insight into effects of long-chain fatty acids on propionic acid production in anaerobic fermentation:A case study of oleic acid and palmitic acid[J]. Journal of Water Process Engineering, 2021,44:102415.
[6]	Sousa D Z, Smidt H, Alves M M, et al. Ecophysiology of syntrophic communities that degrade saturated and unsaturated long-chain fatty acids[J]. FEMS microbiology ecology, 2009,68(3):257-272.
[7]	Novak J T, Carlson D A. The kinetics of anaerobic long chain fatty acid degradation[J]. Journal (Water Pollution Control Federation), 1970:1932-1943.
[8]	Hanaki K, Matsuo T, Nagase M. Mechanism of inhibition caused by long-chain fatty acids in anaerobic digestion process[J]. Biotechnology and bioengineering, 1981,23(7):1591-1610.
[9]	Zhu K, Zhang L, Mu L, et al. Comprehensive investigation of soybean oil-derived LCFAs on anaerobic digestion of organic waste:Inhibitory effect and transformation[J]. Biochemical Engineering Journal, 2019, 151:107314.
[10]	Eftaxias A, Diamantis V, Michailidis C, et al. Comparison of anaerobic digesters performance treating palmitic, stearic and oleic acid:determination of the LCFA kinetic constants using ADM1[J]. Bioprocess and biosystems engineering, 2020,43:1329-1338.
[11]	Li Q, Liu Y, Yang X, et al. Kinetic and thermodynamic effects of temperature on methanogenic degradation of acetate, propionate, butyrate and valerate[J]. Chemical Engineering Journal, 2020,396:125366.
[12]	Wei W, Huang Q S, Sun J, et al. Revealing the mechanisms of polyethylene microplastics affecting anaerobic digestion of waste activated sludge[J]. Environmental science & technology, 2019, 53(16):9604-9613.
[13]	鲁斌,龚凯,蒋红与,等.AnMBR用于餐厨垃圾和剩余污泥共发酵的性能研究[J].中国环境科学, 2021,41(5):2290-2298. Lu B, Gong K, Jiang H Y, et al. Performance of AnMBR for the co-digestion of food waste and waste activity sludge[J]. China Environmental Science, 2021,41(5):2290-2298.
[14]	Neves L, Pereira M A, Mota M, et al. Detection and quantification of long chain fatty acids in liquid and solid samples and its relevance to understand anaerobic digestion of lipids[J]. Bioresource Technology, 2009,100(1):91-96.
[15]	Li Q, Liu Y, Gao W, et al. New insights into the mechanisms underlying biochar-assisted sustained high-efficient co-digestion:Reducing thermodynamic constraints and enhancing extracellular electron transfer flux[J]. Science of The Total Environment, 2022,811:151416.
[16]	Li Q, Gao X, Liu Y, et al. Biochar and GAC intensify anaerobic phenol degradation via distinctive adsorption and conductive properties[J]. Journal of Hazardous Materials, 2021,405:124183.
[17]	Kalayasiri P, Jeyashoke N, Krisnangkura K. Survey of seed oils for use as diesel fuels[J]. Journal of the American Oil Chemists'Society, 1996,73(4):471-474.
[18]	Hidalgo D, Martín-Marroquín J M, Corona F. The effect of feed composition on anaerobic co-digestion of animal-processing by-products[J]. Journal of Environmental Management, 2018,216:105-110.
[19]	Wu L J, Kobayashi T, Li Y Y, et al. Determination and abatement of methanogenic inhibition from oleic and palmitic acids[J]. International Biodeterioration & Biodegradation, 2017,123:10-16.
[20]	Wu L J, Hao Z W, Li X X, et al. Excess methane production and operation stability for anaerobic digestion of oily food waste controlled by mixing intensity:Focusing on heterogeneity of long chain fatty acids[J]. Journal of Environmental Management, 2023,335:117573.
[21]	Fujihira T, Seo S, Yamaguchi T, et al. High-rate anaerobic treatment system for solid/lipid-rich wastewater using anaerobic baffled reactor with scum recovery[J]. Bioresource Technology, 2018,263:145-152.
[22]	Grabowski A, Tindall B J, Bardin V, et al. Petrimonassulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir[J]. International journal of systematic and evolutionary microbiology, 2005,55(3):1113-1121.
[23]	Liu Y, He P, Duan H, et al. Low calcium dosage favors methanation of long-chain fatty acids[J]. Applied Energy, 2021,285:116421.
[24]	Gerritsen J, Fuentes S, Grievink W, et al. Characterization of Romboutsiailealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov[J]. International journal of systematic and evolutionary microbiology, 2014,64(Pt5):1600-1616.
[25]	Zhang C, Liu X, Dong X. Syntrophomonascurvata sp. nov., an anaerobe that degrades fatty acids in co-culture with methanogens[J]. International Journal of Systematic and Evolutionary Microbiology, 2004,54(3):969-973.
[26]	Conklin A, Stensel H D, Ferguson J. Growth kinetics and competition between Methanosarcina and Methanosaeta in mesophilic anaerobic digestion[J]. Water environment research, 2006,78(5):486-496.
[27]	Sharma P, Khardenavis A A, Purohit H J. Metabolism of long-chain fatty acids (LCFAs) in methanogenesis[J]. Microbial Factories:Biofuels, Waste treatment:Volume 1, 2015:279-291.
[28]	Cavaleiro A J, Pereira M A, Guedes A P, et al. Conversion of Cn-unsaturated into Cn-2-saturated LCFA can occur uncoupled from methanogenesis in anaerobic bioreactors[J]. Environmental Science & Technology, 2016,50(6):3082-3090.
[29]	Kurade M B, Saha S, Salama E S, et al. Acetoclastic methanogenesis led by Methanosarcina in anaerobic co-digestion of fats, oil and grease for enhanced production of methane[J]. Bioresource technology, 2019,272:351-359.