主流条件下间歇曝气抑制NOB与微生物动态响应

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1144 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The key conditions and inhibition mechanism in nitrification process were analysed by using different sludge sources and anoxic/aerobic ratios of intermittent aeration process under mainstream conditions. The results showed that nitrite accumulation was difficult to obtain in partial nitrification system relying only under low-dissolve oxygen (DO) and intermittent aeration. When coupled with anammox process, NO₃^--N concentration in effluent decreased from 41.64mg/L to 11.54mg/L under the same NH₄⁺-N conversion environment, and the nitrification process was significantly inhibited. The quantitative analysis shows that Nitrate-oxidizing bacteria (NOB) had strong adaptability in the environment of low-DO and intermittent aeration, with a maximum increase of 2.9 times after 15days of operation, while in a mixed system with anammox bacteria, the maximum increase was only 1.4 times. The results of microbial community structure change showed that in low DO coupled intermittent aeration environment, the abundance of NOB inoculated in mixed sludge system increased faster than that in nitrification sludge system. Therefore, strengthening nitrite utilization and reducing its exposure concentration in aerobic stage is a key condition for achieving NOB inhibition in intermittent aeration process.

Key words： partial nitrification intermittent aeration inhibition mechanism inoculation sludge source anoxic/aerobic ratio

Received: 07 February 2023

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XIE Yi-yi
	LI Xiang
	YUAN Yi
	XU Pei-ling
	HUANG Yong
	WU Peng

Cite this article:

XIE Yi-yi,LI Xiang,YUAN Yi等. The way of coupling intermittent aeration strategy to inhibit NOB activity in mainstream environment and the dynamic response of microorganisms[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4616-4625.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4616

[1]	Cao Y, Van Loosdrecht M C M, Daigger G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017,101(4):1365-1383.
[2]	Zhang M, Wang S, J I B, et al. Towards mainstream deammonification of municipal wastewater:Partial nitrification-anammox versus partial denitrification-anammox[J]. Science of The Total Environment, 2019,692:393-401.
[3]	Ma W J, Li G F, Huang B C, et al. Advances and challenges of mainstream nitrogen removal from municipal wastewater with anammox-based processes[J]. Water Environment Research, 2020, 92(11):1899-1909.
[4]	Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences-An application survey[J]. Water Research, 2014,55:292-303.
[5]	Wang Z, Peng Y, Miao L, et al. Continuous-flow combined process of nitritation and ANAMMOX for treatment of landfill leachate[J]. Bioresource Technology, 2016,214:514-519.
[6]	Izadi P, Izadi P, Eldyasti A. Towards mainstream deammonification:Comprehensive review on potential mainstream applications and developed sidestream technologies[J]. Journal of Environmental Management, 2021,279:111615.
[7]	Duan H, Ye L, Lu X, et al. Overcoming nitrite oxidizing bacteria adaptation through alternating sludge treatment with free nitrous acid and free ammonia[J]. Environmental Science & Technology, 2019, 53(4):1937-1946.
[8]	Lotti T, Kleerebezem R, HU Z, et al. Pilot-scale evaluation of anammox-based mainstream nitrogen removal from municipal wastewater[J]. Environmental Technology, 2015,36(9):1167-1177.
[9]	Xiang T, Gao D, Wang X. Performance and microbial community analysis of two sludge type reactors in achieving mainstream deammonification with hydrazine addition[J]. Science of The Total Environment, 2020,715:136377.
[10]	Wang G, Zhang D, Xu Y, et al. Comparing two start up strategies and the effect of temperature fluctuations on the performance of mainstream anammox reactors[J]. Chemosphere, 2018,209:632-639.
[11]	Akaboci T R V, Gich F, Ruscalleda M, et al. Assessment of operational conditions towards mainstream partial nitritation-anammox stability at moderate to low temperature:Reactor performance and bacterial community[J]. Chemical Engineering Journal, 2018,350:192-200.
[12]	Gu J, Zhang M, Wang S, et al. Integrated upflow anaerobic fixed-bed and single-stage step-feed process for mainstream deammonification:A step further towards sustainable municipal wastewater reclamation[J]. Science of The Total Environment, 2019,678:559-564.
[13]	Laureni M, Weissbrodt D G, Villez K, et al. Biomass segregation between biofilm and flocs improves the control of nitrite-oxidizing bacteria in mainstream partial nitritation and anammox processes[J]. Water Research, 2019,154:104-116.
[14]	Wett B, Podmirseg S M, Gómez-Brandón M, et al. Expanding DEMON sidestream deammonification technology towards mainstream application[J]. Water Environment Research, 2015,87(12):2084-2089.
[15]	Wang Z, Zheng M, Hu Z, et al. Unravelling adaptation of nitrite-oxidizing bacteria in mainstream PN/A process:Mechanisms and counter-strategies[J]. Water Research, 2021,200:117239.
[16]	Li J, Li J, Gao R, et al. A critical review of one-stage anammox processes for treating industrial wastewater:Optimization strategies based on key functional microorganisms[J]. Bioresource Technology, 2018,265:498-505.
[17]	Qiu S, Li Z, Hu Y, et al. What's the best way to achieve successful mainstream partial nitritation-anammox application?[J]. Critical Reviews in Environmental Science and Technology, 2021,51(10):1045-1077.
[18]	Li X, Yuan Y, Huang Y, et al. Inhibition of nitrite oxidizing bacterial activity based on low nitrite concentration exposure in an auto-recycling PN-Anammox process under mainstream conditions[J]. Bioresour Technol, 2019,281:303-308.
[19]	Picioreanu C, Van Loosdrecht M C M, Heijnen J J. Modelling the effect of oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor[J]. Water Science and Technology, 1997, 36(1):147-156.
[20]	Blackburne R, Yuan Z, Keller J. Partial nitrification to nitrite using low dissolved oxygen concentration as the main selection factor[J]. Biodegradation, 2008,19(2):303-312.
[21]	Joss A, Derlon N, Cyprien C, et al. Combined nitritation-anammox:advances in understanding process stability[J]. Environmental Science & Technology, 2011,45(22):9735-9742.
[22]	Xu Z, Zhang L, Gao X, et al. Optimization of the intermittent aeration to improve the stability and flexibility of a mainstream hybrid partial nitrification-anammox system[J]. Chemosphere, 2020,261:127670.
[23]	Salem S, Moussa M S, Van Loosdrecht M C M. Determination of the decay rate of nitrifying bacteria[J]. Biotechnology and Bioengineering, 2006,94(2):252-262.
[24]	Xu G, Xu X, Yang F, et al. Partial nitrification adjusted by hydroxylamine in aerobic granules under high DO and ambient temperature and subsequent Anammox for low C/N wastewater treatment[J]. Chemical Engineering Journal, 2012,213:338-345.
[25]	Malovanyy A, Yang J, Trela J, et al. Combination of upflow anaerobic sludge blanket (UASB) reactor and partial nitritation/anammox moving bed biofilm reactor (MBBR) for municipal wastewater treatment[J]. Bioresource Technology, 2015,180:144-153.
[26]	Kornaros M, Dokianakis S N, Lyberatos G. Partial nitrification/denitrification can be attributed to the slow response of nitrite oxidizing bacteria to periodic anoxic disturbances[J]. Environmental Science & Technology, 2010,44(19):7245-7253.
[27]	Miao Y, Zhang L, Yang Y, et al. Start-up of single-stage partial nitrification-anammox process treating low-strength swage and its restoration from nitrate accumulation[J]. Bioresource Technology, 2016,218:771-779.
[28]	Qiu S, Hu Y, Liu R, et al. Start up of partial nitritation-anammox process using intermittently aerated sequencing batch reactor:Performance and microbial community dynamics[J]. Science of The Total Environment, 2019,647:1188-1198.
[29]	Gustavsson D J I, Suarez C, Wilén B M, et al. Long-term stability of partial nitritation-anammox for treatment of municipal wastewater in a moving bed biofilm reactor pilot system[J]. Science of the Total Environment, 2020,714:136342.
[30]	Liu W, Liu C, Zhang S, et al. Initial nitrite concentration promote nitrite-oxidizing bacteria activity recovery from transient anoxia:Experimental and modeling investigations[J]. Bioresource Technology, 2019,289:121711.
[31]	Li X, Yuan Y, Yuan Y, et al. Effects of salinity on the denitrification efficiency and community structure of a combined partial nitritation-anaerobic ammonium oxidation process[J]. Bioresource Technology, 2018,249:550-556.
[32]	Apha A, and Wef. Standard methods for the examination of water and wastewater, 21st ed.[J]. American Public Health Association, Washington, DC, 2005:
[33]	Li B, Wang Y, LI J, et al. The symbiosis of anaerobic ammonium oxidation bacteria and heterotrophic denitrification bacteria in a size-fractioned single-stage partial nitrification/anammox reactor[J]. Biochem Eng J, 2019,151:107353.
[34]	Katsogiannis A N, Kornaros M, Lyberatos G. Enhanced nitrogen removal in SBRs bypassing nitrate generation accomplished by multiple aerobic/anoxic phase pairs[J]. Water Science and Technology, 2003,47(11):53-59.
[35]	Cui Y, Gao J, WU Z, et al. Inhibition of nitrospira and nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater[J]. Chemical Engineering Journal, 2023,454:140139.
[36]	Weißbach M, Drewes J E, Koch K. Application of the oxidation reduction potential (ORP) for process control and monitoring nitrite in a coupled aerobic-anoxic nitrous decomposition operation (CANDO)[J]. Chemical Engineering Journal, 2018,343:484-491.
[37]	Li J, Elliott D, Nielsen M, et al. Long-term partial nitrification in an intermittently aerated sequencing batch reactor (SBR) treating ammonium-rich wastewater under controlled oxygen-limited conditions[J]. Biochem Eng J, 2011,55(3):215-222.
[38]	Miao Y, Zhang L, Yu D, et al. Application of intermittent aeration in nitrogen removal process:development, advantages and mechanisms[J]. Chemical Engineering Journal, 2022,430:133184.
[39]	Chen R, Ji J, Chen Y, et al. Successful operation performance and syntrophic micro-granule in partial nitritation and anammox reactor treating low-strength ammonia wastewater[J]. Water Research, 2019, 155:288-299.
[40]	Miao Y, Peng Y, Zhang L, et al. Partial nitrification-anammox (PNA) treating sewage with intermittent aeration mode:Effect of influent C/N ratios[J]. Chemical Engineering Journal, 2018,334:664-672.
[41]	Chen Y, Zhao Z, Liu H, et al. Achieving stable two-stage mainstream partial-nitrification/anammox (PN/A) operation via intermittent aeration[J]. Chemosphere, 2020,245:125650.
[42]	Pérez J, Lotti T, Kleerebezem R, et al. Outcompeting nitrite-oxidizing bacteria in single-stage nitrogen removal in sewage treatment plants:A model-based study[J]. Water Research, 2014,66:208-218.
[43]	Sun Y, Guan Y, Wang H, et al. Autotrophic nitrogen removal in combined nitritation and Anammox systems through intermittent aeration and possible microbial interactions by quorum sensing analysis[J]. Bioresource Technology, 2019,272:146-155.
[44]	Liu H, Peng Y, Lu J, et al. Effect of the ratio of intermittent aeration on activity of nitrifying bacteria in shortcut nitrification[J]. Environmental Pollution & Control, 2017,39(12):1317-1321.