pH值调控方法对剩余污泥厌氧发酵的影响

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Abstract This experiment focuses on the wasted sludge from municipal sewage treatment plants. Batch texts were taken to explore the effects of different pH regulation methods, which include constant pH adjustment with values of 5,7,9,10 and phased pH adjustment, on the dissolution of organic matter in anaerobic fermentation. The variations of microbial community structure of fermentation sludge under different pH adjustment conditions were analyzed by High-throughput sequencing technology. Results showed that more organic matter could be dissolved under both alkaline and acidic conditions with constant pH adjustment, and the accumulation reached the maximum when pH value was 10. At this point, solluted chemical oxigen demand (SCOD) concentration was 323.77mg/gVSS, and the VFAs was 183.38mgCOD/gVSS. In the case of phased pH adjustment, a constant pH of 10was controlled only in the initial stage of fermentation (the first 4days) and the pH value was naturally reduced in the later stage, this method showed more conducive to the production of VFAs in the acid production stage. This maximum yield was 208.78mgCOD/gVSS, which was 13.90% higher than the maximum total VFAs of same pH value but with constant pH adjustment. At the phylum level, significant differences in microbial abundance appeared after different pH adjustment operations , but the main dominant microorganisms were still Firmicutes, Proteobacteria, Actinobacteriota and Chloroflexi. Under constant pH regulation, the relative abundance of Firmicutes and Actinobacteriota reached 61.14% and 15.57% when pH value was 10, which was the highest beyond other pH values. As for the phased pH regulation, the relative abundance of Firmicutes and Actinobacteriota increased to 68.46% and 18.43% respectively , which grew more proportion than the condition of constant pH adjustment mentioned above. At the genus level, the bacterial abundance related to hydrolytic acidification (Proteiniclasticum, Bacillus, Acetoanaerobium, Proteocatella and Alkaliphilus, etc.) accounted for 32.38% when pH value was constantly 10, and 51.62% under phased regulation. The phased pH regulation improved the relative abundance of hydrolytic acidifying bacteria and was more contributive to the accumulation of VFAs during fermentation.

Key words： anaerobic fermentation volatile fatty acid (VFA) pH high-throughput sequencing

Received: 17 February 2023

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X703

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	Articles by authors
	BI Hao-hua
	GAO Chun-di
	LIU Yi-wei
	XING Yi-yan
	PENG Yong-zhen

Cite this article:

BI Hao-hua,GAO Chun-di,LIU Yi-wei等. effects of pH control methods on anaerobic fermentation of waste activated sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4648-4657.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4648

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