接种污泥和pH值对CMC-Na厌氧降解的影响及菌群解析

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Abstract In order to reveal the anaerobic degradation efficiency of sodium carboxymethyl cellulose (CMC-Na), the effects of different types of inoculation sludge and initial pH on the anaerobic degradation of CMC-Na were investigated by batch culture. The response of microbial community on initial pH was revealed by Illumina MiSeq sequencing. The results showed that the flocculent sludge had strong hydrolysis and fermentation acid-producing capacity under the initial CMC-Na concentration of 5g/L condition. However, this system was unfavorable for syntrophic acetogenesis and methanogenesis, resulting in the methane yield was reached 10.0mL/g CMC-Na. On the contrary, the high methane fermentation capacity of the granular sludge made the methane yield reached 100.2mL/g CMC-Na. The effects of initial pH on anaerobic degradation of CMC-Na showed that the methane production was significantly inhibited by initial pH 4.5 and 9.5. However, the methane yield reached the maximum (123.4 and 123.2mL/g CMC-Na) at the initial pH 7.5 and 8.5. Illumina MiSeq sequencing showed that the microbial composition in different initial pH conditions was significantly different. At initial pH 7.5, Metanothrix and Metanobacterium were the dominant methanogens, while Macellibacteroides, Petromonas, Trichococcus, Anaerofilum and Brooklawnia were the dominant fermentation acid-producing bacteria. When the initial pH was 4.5 or 9.5, Metanosarcina also became a dominant genus, and the relative abundance of the main fermentation acid-producing bacteria was changed significantly.

Key words： sodium carboxymethyl cellulose inoculated sludge initial pH anaerobic degradation microbial community

Received: 09 January 2023

PACS:

X703.5

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	Articles by authors
	BAN Qiao-ying
	ZHANG Si-yu
	WANG Yang
	ZHANG Li-guo
	YAN Rui-feng

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BAN Qiao-ying,ZHANG Si-yu,WANG Yang等. Effect of inoculated sludge and pH on the anaerobic degradation of sodium carboxymethyl cellulose as well as microbial community analysis[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4658-4665.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4658

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