自养硝化颗粒污泥培养过程及微观特征

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Abstract In this study, mature aerobic granular sludge and flocculation activated sludge were both used as inoculation sludge in sequencing batch reactor (SBR) to rapidly culture ANGS. The experimental results showed that the addition of mature aerobic granular sludge is conducive to the rapid formation of ANGS and quick start-up of SBR. Aerobic granular sludge can provide the attachment carrier for nitrifying bacteria, and rapidly cultivate ANGS with good ammonia nitrogen removal performance. Extracellular polymeric substances (EPS) plays an important role in the formation process of ANGS, and tightly bound-EPS (TB-EPS) had the highest protein and polysaccharide contents, they reached 192.197 and 59.380mg/g vss, indicating that the microbial communication was intensive TB-EPS layer, and TB-EPS is beneficial to the formation of ANGS. Hydrophobic tryptophan and tyrosine up to 70% can provide more hydrophobic adsorption sites for soluble microbial products-EPS (SMP-EPS) and loosely bound-EPS (LB-EPS), thereby enhancing the adhesion of the sludge particles and promoting the formation of ANGS. In addition, the increased proportion of humic acid and soluble microbial metabolites in TB-EPS are beneficial to enhance the flocculation ability of ANGS. Glutamic acid, as the crossing point between carbon and nitrogen metabolism, accounts for 11.18% to 13.64%, is the basic amino acid of nitrogen metabolism, and the increase of proline content is helpful to maintain the stability of biofilm structure. The favorable aggregation structures including a-helix, b-angle and reverse b-folding of protein secondary structures in the amide Ⅰ region were much higher than the unfavorable aggregation structures, indicating that the stability of ANGS was enhanced during the granulation process.

Key words： autotrophic nitrifying granular sludge extracellular polymeric substances 3D fluorescence fourier transform infrared spectroscopy protein secondary structure amino acids

Received: 09 January 2023

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X703

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	Articles by authors
	LIU Chong
	LI Yu-guang
	CHEN Ning
	ZHANG Xin
	SHEN Fei
	WANG Shuo
	LI Ji

Cite this article:

LIU Chong,LI Yu-guang,CHEN Ning等. Formation process and microscopic characteristics of autotrophic nitrifying granular sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4688-4696.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4688

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