硝化颗粒污泥胞外聚合物及信号分子功能分析

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Abstract Autotrophic nitrifying granular sludge (ANGS) was cultivated using activated sludge as the inoculated sludge in SBR. The cultivation of autotrophic nitrifying granular sludge was relatively long and the control of granulation process was difficult, however, ANGS with good sedimentation property can be obtained, and ammonia nitrogen removal was higher than 90%. TB-EPS had the highest proportion of protein and polysaccharide, and the respective content of TB-PN and TB-PS were 90.69mg/gVSS and 53.69mg/gVSS on the 70th day. As the core of sludge particle structure, TB-EPS played an important role in the granulation process. Hydrophobic tryptophan and tyrosine are beneficial to the SMP layer and LB-EPS to enhance the adhesion between the sludge, thereby promoting the formation of autotrophic nitrifying granular sludge. The relative proportion of 8hydrophobic amino acids increased from the initial 34% to the highest 66%, indicating that hydrophobic amino acids play an important role in the critical period of granulation process. Polymerase chain can enhance the binding force between sludge particles, and β- folding structure was able to provide more hydrophobic sites to promote the accumulation of the sludge particles, which was favorable to the formation of ANGS. The concentration of C4-HSL reached 112.00ng/L, and it could combine with LuxR protein to promote protease activity and EPS production. C6-HSL and C8-HSL could regulate the biofilm formation of nitrification bacteria. C6-HSL was up to 30μg/L on the 30th day, and its content was the highest, indicating that it plays an important role in the signal transmission of autotrophic nitrification granular sludge. 3OC6-HSL was positively correlated with protein substances, and its correlation coefficients with SMP-PN and LB-PN were 0.77 and 0.71, which may enhance the adhesion of sludge by guiding the synthesis of protein substances, thus facilitate the aggregation of sludge particles.

Key words： autotrophic nitrifying granular sludge extracellular polymeric substances (EPS) EPS fluorescence spectrum protein secondary structure EPS amino acids AHLs

Received: 10 February 2023

PACS:	X703
	X172

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	Articles by authors
	CHEN Ning
	LIU Chong
	LI Yu-guang
	ZHANG Xin
	SHEN Fei
	WANG Shuo
	LI Ji

Cite this article:

CHEN Ning,LIU Chong,LI Yu-guang等. Characteristics of extracellular polymer substances and signal molecule function in autotrophic nitrifying granular sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(9): 4666-4675.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I9/4666

[1]	Ali M, Chai L Y, Min X B, et al. Performance and characteristics of a nitritation air-lift reactor under long-term HRT shortening[J]. International Biodeterioration & Biodegradation, 2016,111:45-53.
[2]	Cydzik-Kwiatkowska A, Wojnowska-Baryla I. Nitrifying granules cultivation in a sequencing batch reactor at a low organics-to-total nitrogen ratio in wastewater[J]. Folia Microbiol. (Praha), 2011,56(3):201-208.
[3]	Rathnayake R M, Song Y, Tumendelger A, et al. Source identification of nitrous oxide on autotrophic partial nitrification in a granular sludge reactor[J]. Water Res, 2013,47(19):7078-86.
[4]	Belmonte M, Vazquez-Padin J R, Figueroa M, et al. Characteristics of nitrifying granules developed in an air pulsing SBR[J]. Process Biochemistry, 2009,44(5):602-606.
[5]	Tsuneda S, Nagano T, Hoshino T, et al. Characterization of nitrifying granules produced in an aerobic upflow fluidized bed reactor[J]. Water Res, 2003,37(20):4965-73.
[6]	张子健,吴伟伟,王建龙.全自养硝化污泥的颗粒化过程研究[J]. 环境科学, 2010,31(1):140-146. Zhang Z J, Wu W W, Wang J L. Study on granulation process of panautotrophic nitrification sludge[J]. Environmental Science, 2010,31(1):140-146.
[7]	Tay J H, Yang S F, Liu Y. Hydraulic selection pressure-induced nitrifying granulation in sequencing batch reactors[J]. Appl. Microbiol. Biotechnol., 2002,59(2/3):332-337.
[8]	李志华,张婷,吴杰,等.异养菌与自养菌对好氧颗粒污泥稳定性的影响[J]. 土木建筑与环境工程, 2010,32(5):76-81. Li Z H, Zhang T, Wu J, et al. Effects of heterotrophic bacteria and autotrophic bacteria on the stability of aerobic granular sludge[J]. Civil, Architecture and Environmental Engineering, 2010,32(5):76-81.
[9]	Wang J, Qian F, Liu X, et al. Cultivation and characteristics of partial nitrification granular sludge in a sequencing batch reactor inoculated with heterotrophic granules[J]. Appl. Microbiol. Biotechnol., 2016, 100(21):9381-9391.
[10]	侯保连,李安婕,孙趣.AHLs群体感应信号分子对硝化污泥附着生长及硝化效果的影响[J]. 环境科学学报, 2015,35(9):2773-2779. Hou B L, Li A J, Sun Q. Effect of AHLs quorum sensing signal molecules on adhesion growth and nitrification effect of nitrifying sludge[J]. Journal of Environmental Sciences, 2015,35(9):2773-2779.
[11]	Chen X T, Zhao B H, Zhang J, et al. Rapid start-up of partial nitrification reactor by exogenous AHLs and Vanillin combined with intermittent aeration[J]. Sci Total Environ, 2023,859(Pt 1):160191.
[12]	Su B, Qu Z, Song Y, et al. Investigation of measurement methods and characterization of zeta potential for aerobic granular sludge[J]. Journal of Environmental Chemical Engineering, 2014,2(2):1142-1147.
[13]	Shi X Y, Sheng G P, Li X Y, et al. Operation of a sequencing batch reactor for cultivating autotrophic nitrifying granules[J]. Bioresour. Technol., 2010,101(9):2960-2964.
[14]	Zhang B, Long B, Cheng Y, et al. Rapid domestication of autotrophic nitrifying granular sludge and its stability during long-term operation[J]. Environ. Technol., 2021,42(16):2587-2598.
[15]	Ni B J, Xie W M, Chen Y P, et al. Heterotrophs grown on the soluble microbial products (SMP) released by autotrophs are responsible for the nitrogen loss in nitrifying granular sludge[J]. Biotechnol. Bioeng., 2011,108(12):2844-2852.
[16]	Zhang L, Long B, Cheng Y, et al. Rapid cultivation and stability of autotrophic nitrifying granular sludge[J]. Water Sci. Technol., 2020, 81(2):309-320.
[17]	Liu Y Q, Cinquepalmi S. Exploration of mechanisms for calcium phosphate precipitation and accumulation in nitrifying granules by investigating the size effects of granules[J]. Water Res., 2021,206:117753.
[18]	Peng T, Wang Y, Wang J, et al. Effect of different forms and components of EPS on sludge aggregation during granulation process of aerobic granular sludge[J]. Chemosphere, 2022,303(Pt 2):135116.
[19]	Chen H, Li A, Cui C, et al. AHL-mediated quorum sensing regulates the variations of microbial community and sludge properties of aerobic granular sludge under low organic loading[J]. Environ. Int., 2019, 130:104946.
[20]	Geyik A G, Kilic B, Cecen F. Extracellular polymeric substances (EPS) and surface properties of activated sludges:effect of organic carbon sources[J]. Environ. Sci. Pollut. Res. Int., 2016,23(2):1653-63.
[21]	He X S, Xi B D, Li X, et al. Fluorescence excitation-emission matrix spectra couped with parallel factor and regional integration analysis to characterize rganic matter humification[J]. Chemosphere, 2013,93(9):2208-15.
[22]	Rollemberg S L D, Dos Santos A F, Ferreira T J T, et al. Evaluation of the production of alginate-like exopolysaccharides (ALE) and tryptophan in aerobic granular sludge systems[J]. Bioprocess and Biosystems Engineering, 2021,44(2):259-270.
[23]	Zhang W, Zhou X, Cao X, et al. Accelerating anammox nitrogen removal in low intensity ultrasound-assisted ASBBR:Performance optimization, EPS characterization and microbial community analysis[J]. Sci. Total Environ., 2022,817:152989.
[24]	Ni L, Li D, Rong S, et al. Characterization of extracellular polymeric substance (EPS) fractions produced by Microcystis aeruginosa under the stress of linoleic acid sustained-release microspheres[J]. Environ. Sci. Pollut. Res. Int., 2017,24(26):21091-21102.
[25]	Zhu Y, Xiao K, Zhou Y, et al. Profiling of amino acids and their interactions with proteinaceous compounds for sewage sludge dewatering by Fenton oxidation treatment[J]. Water Res., 2020, 175:115645.
[26]	王珏.典型兽用抗生素对脲酶和β-葡萄糖苷酶的影响及作用机制[D]. 南京:南京农业大学, 2020. Wang J. Effects of typical veterinary antibiotics on urease and β-glucosidase and its mechanism of action[D]. Nanjing:Nanjing Agricultural University, 2020.
[27]	Hayat S, Hayat Q, Alyemeni M N, et al. Role of proline under changing environments:a review[J]. Plant Signal Behav., 2012, 7(11):1456-66.
[28]	Wang S, Chen M, Zheng K, et al. Promising carbon utilization for nitrogen recovery in low strength wastewater treatment:Ammonia nitrogen assimilation, protein production and microbial community structure[J]. Sci. Total Environ., 2020,710:136306.
[29]	Rosa-Masegosa A, Perez-Bou L, Munoz-Palazon B, et al. Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors[J]. Amino. Acids, 2022,54:1403-1419.
[30]	Xue Y, Zheng M, Wu S, et al. Changes in the Species and Functional Composition of Activated Sludge Communities Revealed Mechanisms of Partial Nitrification Established by Ultrasonication[J]. Front. Microbiol., 2022,13:960608.
[31]	Chen Y P, Li C, Guo J S, et al. Extraction and characterization of extracellular polymeric substances in biofilm and sludge via completely autotrophic nitrogen removal over nitrite system[J]. Appl Biochem. Biotechnol., 2013,169(2):526-38.
[32]	Yang Y J, Wang B, Guo X J, et al. Investigating adsorption performance of heavy metals onto humic acid from sludge using Fourier-transform infrared combined with two-dimensional correlation spectroscopy[J]. Environ. Sci. Pollut. Res. Int., 2019, 26(10):9842-9850.
[33]	Wang B, Guo X. Reuse of waste beer yeast sludge for biosorptive decolorization of reactive blue 49 from aqueous solution[J]. World J Microbiol. Biotechnol., 2011,27(6):1297-302.
[34]	Gong Y F, Song J, Ren H T, et al. Comparison of Cr(VI) removal by activated sludge and dissolved organic matter (DOM):importance of UV light[J]. Environ. Sci. Pollut. Res. Int., 2015,22(23):18487-94.
[35]	Xu X, Liu J, Sun H. Improving granular sludge stability via stimulation of extracellular polymeric substance production by adding layered double hydroxides[J]. International Journal of Environmental Science and Technology, 2019,16(2):987-994.
[36]	郭致蓥.改性玉米芯粉强化污泥脱水及机理研究[D]. 昆明:昆明理工大学, 2021. Guo Z Y. Study on modified corncob powder to enhance sludge dewatering and its mechanism[D]. Kunming:Kunming University of Science and Technology, 2021.
[37]	高诗卉,王毅力.Fe~0/Fe3C@CS激发PMS调理对活性污泥脱水性能的影响[J]. 环境工程学报, 2020,14(7):1915-1923. Gao S H, Wang Y L. Effect of PMS conditioning induced by Fe~0/Fe3C@CS on dewatering performance of activated sludge[J]. Journal of Environmental Engineering, 2020,14(7):1915-1923.
[38]	Sang W J, Li X Y, Feng Y J, et al. Improvement of the sludge flocculation dewatering efficient by electromagnetic wave loading:research based on removal of bound water[J]. Environmental Science and Pollution Research, 2020,27(3):3413-3427.
[39]	莫志华,梁嘉林,关智杰,等.零价铁活化过一硫酸盐联合单宁酸调理改善市政污泥脱水性能的机理研究环境科学学报[J]. 2022,42(6):121-137. Mo Z H, Liang J L, Guan Z J, et al. Study on the mechanism of zero-valent iron activation of permonosulfate combined with tannic acid to improve the dewatering performance of municipal sludge[J]. Chinese Journal of Environmental Sciences, 2022,42(6):121-137.
[40]	孙梦格,宋卫锋,杨佐毅,等.Cd(Ⅱ)胁迫/诱导下铜绿假单胞菌EPS组分变化及其吸附性能[J]. 环境科学学报, 2021,41(9):3427-3436. Sun M G, Song W F, Yang Z Y, et al. Changes of EPS components and adsorption properties of Pseudomonas aeruginosa under Cd(Ⅱ) stress/induction[J]. Journal of Environmental Sciences, 2021,41(9):3427-3436.
[41]	Sun Z, Xi J, Yang C, et al. Quorum sensing regulation methods and their effects on biofilm in biological waste treatment systems:A review[J]. Frontiers of Environmental Science & Engineering, 2021, 16(7):157-185.
[42]	Li Y S, Cao J S, Yu H Q. Impacts of environmental factors on AHL-producing and AHL-quenching activities of aerobic granules[J]. Appl. Microbiol. Biotechnol., 2019,103(21/22):9181-9189.
[43]	Zhu Y L, Hou H M, Zhang G L, et al. AHLs regulate biofilm formation and swimming motility of Hafnia alvei H4[J]. Front. Microbiol., 2019,10:1330.
[44]	Li A J, Hou B L, Li M X. Cell adhesion, ammonia removal and granulation of autotrophic nitrifying sludge facilitated by N-acyl-homoserine lactones[J]. Bioresour. Technol., 2015,196:550-558.
[45]	Sun Y, Guan Y, Wang D, et al. Potential roles of acyl homoserine lactone based quorum sensing in sequencing batch nitrifying biofilm reactors with or without the addition of organic carbon[J]. Bioresour. Technol., 2018,259:136-145.
[46]	Wang J, Liu Q, Dong D, et al. AHLs-mediated quorum sensing threshold and its response towards initial adhesion of wastewater biofilms[J]. Water Res., 2021,194:116925.