调控内生正磷酸盐强化好氧颗粒污泥脱氮除磷

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (905 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract Orthophosphate is an intermediate product of the biological denitrification process of phosphorus aggregation, which affects the activity of denitrifying phosphorus aggregating bacteria (DPAOs) in competition with other heterotrophic bacteria for carbon sources. In this study, four groups of sequencing batch reactors (SBRs) were operated with various endogenous orthophosphate regulating techniques utilizing synthetic water as influent after being inoculated with flocculent activated sludge.The study aimed to investigate the effects of different endogenous orthophosphate regulation methods on the enrichment of denitrifying phosphorus accumulating bacteria (DPAOs) and granulation. The results showed that the granular sludge operated with endogenous orthophosphate effluent reflux exhibited better nitrogen and phosphorus removal efficiency, higher DPAO activity, and good particle sedimentation performance.In particular, compared to granular sludge systems operated in microaerobic mode, those operated in anaerobic/microaerobic (AO) mode demonstrated superior pollutant treatment performance, with COD, TN, and TP removal efficiencies reaching 92.57%, 94.7%, and 97.62%, respectively. This was attributed to multiple factors, such as endogenous orthophosphate stimulation, the synergistic effect of DPAOs and denitrifying glycans bacteria (DGAOs).Periodic regulation of endogenous orthophosphate in the system resulted in a significant increase in the internal carbon source conversion rate of sludge, enhanced activities of DPAOs and DGAOs, and inhibition of heterotrophic denitrifying bacteria (DOHOs). Under a longer cycle operation mode, ammonia oxidizing bacteria (AOB) and DPAOs interacted, while also inhibiting the growth of other heterotrophic microorganisms. The findings of this study provide theoretical support for the practical engineering application of simultaneous short-range nitritation-denitritation phosphorus removal (SPNDPR) process.

Key words： endophytic orthophosphate denitrifying phosphorus removal bacteria simultaneous nitrification and denitrification granular sludgegranular sludge

Received: 24 March 2023

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Dong
	XIE Yi-bo
	GAO Fei-yan
	ZHU Yan-jun
	ZHANG Jie

Cite this article:

LI Dong,XIE Yi-bo,GAO Fei-yan等. Regulating endogenous orthophosphate to initiate a synchronous short-pass nitrification and denitrification phosphorus removal granular sludge process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5139-5147.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I10/5139

[1]	Lu W, Zhang X, Zhang Y, et al. Synergistic simultaneous endogenous partial denitrification/anammox (EPDA) and denitrifying dephosphatation for advanced nitrogen and phosphorus removal in a complete biofilm system[J]. Bioresource Technology, 2022,358:127378.
[2]	Li D, Yang J, Li Y, et al. Research on rapid cultivation of aerobic granular sludge (AGS) with different feast-famine strategies in continuous flow reactor and achieving high-level denitrification via utilization of soluble microbial product (SMP)[J]. Science of The Total Environment. 2021,786:147237.
[3]	狄斐,隋倩雯,高超龙,等.反硝化除磷工艺实现亚硝酸盐积累的参数优化[J]. 中国环境科学, 2022,42(6):2647-2655. Di Fei, Sui Qianwen, Gao Chaolong, et al. Parameter optimization for nitrite accumulation in denitrifying phosphorus removal process[J]. China Environmental Science, 2022,42(6):2647-2655.
[4]	Lu X, Duan H, Oehmen A, et al. Achieving combined biological short-cut nitrogen and phosphorus removal in a one sludge system with side-stream sludge treatment[J]. Water Research, 2021,203:117563.
[5]	Li D, Guo W, Liang D, et al. Rapid start-up and advanced nutrient removal of simultaneous nitrification, endogenous denitrification and phosphorus removal aerobic granular sequence batch reactor for treating low C/N domestic wastewater[J]. Environmental Research, 2022,212:113464.
[6]	Zaman M, Kim M, Nakhla G. Simultaneous partial nitrification and denitrifying phosphorus removal (PNDPR) in a sequencing batch reactor process operated at low DO and high SRT for carbon and energy reduction[J]. Chemical engineering journal (Lausanne, Switzerland:1996), 2021,425:131881.
[7]	李冬,高飞雁,解一博,等.高频交替OLR强化好氧颗粒污泥性能研究[J]. 中国环境科学, 2022,42(8):3635-3642. Li Dong, Gao Feiyan, Xie Yibo, et al. Study on the performance of aerobic granular sludge enhanced by high-frequency alternating organic loading rate[J]. China Environmental Science, 2022,42(8):3635-3642.
[8]	Coma M, Puig S, Balaguer M D, et al. The role of nitrate and nitrite in a granular sludge process treating low-strength wastewater[J]. Chemical Engineering Journal, 2010,164(1):208-213.
[9]	Izadi P, Izadi P, Eldyasti A. Evaluation of PAO adaptability to oxygen concentration change:Development of stable EBPR under stepwise low-aeration adaptation[J]. Chemosphere, 2022,286:131778.
[10]	Wang X, Wang S, Xue T, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77:191-200.
[11]	Hu T, Peng Y, Yuan C, et al. Enhanced nutrient removal and facilitating granulation via intermittent aeration in simultaneous partial nitrification endogenous denitrification and phosphorus removal (SPNEDpr) process[J]. Chemosphere, 2021,285:131443.
[12]	Xu R, Fu Y, Xu Y, et al. Comparing biotransformation of extracellular polymeric substances (EPS) under aerobic and anoxic conditions:Reactivities, components, and bacterial responses[J]. Chemosphere, 2022,296:133996.
[13]	Yuan C, Wang B, Peng Y, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage anaerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/nitrogen[J]. Chemosphere, 2020,257:127097.
[14]	Lin Y, Sun Y, Zhang L, et al. Balancing denitrifying phosphorus-accumulating organisms and denitrifying glycogen-accumulating organisms for advanced nitrogen and phosphorus removal from municipal wastewater[J]. Bioresource Technology, 2023,369:128444.
[15]	Wang X, Zhao J, Yu D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355:560-571.
[16]	Wang X, Wang S, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR)[J]. Water Research, 2016,95:319-329.
[17]	Miao Y, Zhang L, Li B, et al. Enhancing ammonium oxidizing bacteria activity was key to single-stage partial nitrification-anammox system treating low-strength sewage under intermittent aeration condition[J]. Bioresource Technology, 2017,231:36-44.
[18]	Li H, Zhong Y, Huang H, et al. Simultaneous nitrogen and phosphorus removal by interactions between phosphate accumulating organisms (PAOs) and denitrifying phosphate accumulating organisms (DPAOs) in a sequencing batch reactor[J]. Science of The Total Environment, 2020,744:140852.
[19]	Yang Z, Bin L, Huang S, et al. Revealing the stability of aerobic granular sludge in a membrane bioreactor under different DO values by proteomics analysis[J]. Bioresource Technology Reports, 2021, 14:100673.
[20]	Wang S, Huang X, Liu L, et al. Insight into the role of exopolysaccharide in determining the structural stability of aerobic granular sludge[J]. Journal of Environmental Management, 2021, 298:113521.
[21]	Wei S P, Stensel H D, Ziels R M, et al. Partitioning of nutrient removal contribution between granules and flocs in a hybrid granular activated sludge system[J]. Water Research, 2021,203:117514.
[22]	Campo R, Sguanci S, Caffaz S, et al. Efficient carbon, nitrogen and phosphorus removal from low C/N real domestic wastewater with aerobic granular sludge[J]. Bioresource Technology, 2020,305:122961.
[23]	Zhang M, Zhu C, Pan T, et al. Elucidating sludge characteristic, substrate transformation and microbial evolution in a two-sludge denitrifying phosphorus removal system under the impact of HRT[J]. Journal of Environmental Management, 2020,262:110391.
[24]	Zhang M, Wan J, Fan Y, et al. Bioaugmentation for low C/N ratio wastewater treatment by combining endogenous partial denitrification (EPD) and denitrifying phosphorous removal (DPR) in the continuous A2/O-MBBR system[J]. Journal of Environmental Management, 2022,312:114920.
[25]	Wang Y, Wang J, Liu Z, et al. Effect of EPS and its forms of aerobic granular sludge on sludge aggregation performance during granulation process based on XDLVO theory[J]. Science of The Total Environment, 2021,795:148682.
[26]	Xiao H, Peng Y, Zhang Q, et al. Pre-anaerobic treatment enhanced partial nitrification start-up coupled with anammox for advanced nitrogen removal from low C/N domestic wastewater[J]. Bioresource Technology, 2021,337:125434.
[27]	Zhang X, Wang C, Wu P, et al. New insights on biological nutrient removal by coupling biofilm-based CANON and denitrifying phosphorus removal (CANDPR) process:Long-term stability assessment and microbial community evolution[J]. Science of The Total Environment, 2020,730:138952..
[28]	Zhang X, Wu P, Xu L, et al. A novel simultaneous partial nitritation, denitratation and anammox (SPNDA) process in sequencing batch reactor for advanced nitrogen removal from ammonium and nitrate wastewater[J]. Bioresource Technology, 2022,343:126105.