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| Establishment and assessment of a competitive direct enzyme-linked immunosorbent assay (cdELISA) for the determination of cylindrospermopsin |
| YANG Dan, LIU Jin-ping, LAI Shu-yan, XIAO Li-juan, LEI La-mei |
| Department of Ecology, Jinan University, Guangzhou 510632, China |
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Abstract Cylindrospermopsins(CYN) has been widely concerned in the world for its high toxicity to humans and animals. The establishment of a rapid detection method can provide an effective tool for the risk assessment of CYN. Based on the monoclonal antibody N8 against CYN prepared, a direct competitive ELISA has been developed. After optimization of the dilution of HRP-CYN conjugate and N8 monoclonal antibody, the newly developed ELISA exhibited a typical sigmoidal response for CYN at concentrations of 0~200ng/mL, with a wide quantitative range between 0.1 and 10ng/mL by using logit-log method for fitting the curve. The detection limit of the method was calculated to be 0.069ng/mL, which is well below guideline value of 0.7ng/mL CYN recommended by WHO. Recoveries ranging from 80.44% to 125.4% and 52.97% to 155.19% were observed upon analyzing CYN-spiked water samples and Chlorellae diluent, respectively. Moreover, CYN concentrations obtained from our cdELISA method through testing different samples was highly consistent with the Beacon ELISA kit. It was found that CYN can be detected year around in Dashahe reservoir but never beyond the guideline value of 0.7ng/mL. Therefore, the developed ELISA will achieve the rapid detection of CYN in water samples and algae samples and may provide a reliable technical tool for drinking water safety.
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Received: 20 February 2023
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