氯代烃污染深层土壤细菌群落结构及组装机制

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Abstract To study the structural characteristics and assembly mechanism of bacterial community in chlorinated hydrocarbons contaminated soil, unsaturated-zone soil within 2~10m was collected from different contaminated areas. Based on high-throughput sequencing technology, the bacterial community was analyzed and the main drivers, environmental influencing factors and assembly mechanism of the community structure changes were revealed. The results showed that the main driver of bacterial community structure change in the lightly polluted area was soil type. The β-diversity mainly influenced by species replacement with a contribution of 53.9%, and community composition significantly correlated with water-soluble sulfate(R=0.61, P=0.0002) and total organic carbon content(R=0.42, P=0.0005). Furthermore, the main driver of bacterial community structure change in the heavily polluted area was the degree of pollution. The β-diversity mainly influenced by the differences in abundance with a contribution of 52.9%, community composition was significantly correlated with trichloroethylene(R=0.17, P=0.0425), chloroform (R=0.22, P=0.0375), water-soluble sulfate (R=0.36, P=0.0074), total organic carbon (R=0.29, P=0.0168), and total nitrogen content (R=0.20, P=0.0130). Chlorinated hydrocarbons stress narrowed the niche width and reduced the niche overlap index of most species except adaptive ones, and led to an increase in the abundance of Proteobacteria, while that of Actinobacteriota, Firmicutes, and Chloroflexi decreased. In the soil with low pollutant concentration, the bacterial community assembly was dominated by random process, with a contribution of 65.6%. In the soil with high pollutant concentration, the random process decreased to 27.7%, and the assembly was dominated by deterministic process.

Key words： deep soil bacterial community structural characteristics assembly mechanism

Received: 24 February 2023

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	FAN Yan-ling
	GOU Ya-ling
	WANG Hong-qi
	LIU Zeng-jun
	XU He-feng
	YANG Shuo
	LIANG Jing

Cite this article:

FAN Yan-ling,GOU Ya-ling,WANG Hong-qi等. Structure and assembly mechanism of bacterial communities in deep soil contaminated by chlorinated hydrocarbons[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5550-5561.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I10/5550

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