邻苯二甲酸酯降解功能内生菌群的筛选及定殖效能

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Abstract In order to reduce the threat to human health of phthalate-contaminated crops, this study attempted to enrich and domesticate functional endogenous flora with high efficiency and broad spectrum of PAEs degradation directly from PAEs-contaminated vegetables. The optimal degradation conditions and the community structure were studied, and the colonization efficiency of functional endophytic flora in crops was studied by hydroponic system. High-throughput sequencing results showed that at the taxonomic level the bacterial consortium consisted of Proteobacteria (76.57%), Bacteroidetes (21.04%) and Actinobacteria (2.37%), and at the generic taxonomic level the bacterial consortium consisted of Sphingobacterium (33.03%), Delftia(40.61%), Pseudomonas(11.70%), Achromobacter(3.04%) and Rhizobium(6.90%). In the pure culture system of inorganic salts, the bacterial consortium could degrade 97.08% dimethyl phthalate (DMP, 5mg/L), 94.47% diethyl phthalate (DEP, 5mg/L), 98.02% dibutyl phthalate(DBP, 5mg/L) and 44.82% butyl benzyl phthalate (BBP, 5mg/L) within 7d. The single-factor test results showed that the optimal degradation conditions were: pH 7, 25°C, 1% salinity and 5mg/L substrate concentration. Hydroponic experiments of rice showed that the bacterial consortium could colonize in the rice and significantly increased PAEs removal, compared with the control group, the contents of DMP, DEP, DBP and ∑ PAEs in rice decreased by 41.09%, 45.33%, 63.06% and 32.3% within 15d. The colonization of microflora could also increase the root length, plant height and biomass of rice, and promote the growth of rice. The bacterial consortium has a good application prospect in reducing the accumulation of crop PAEs and ensuring the quality and safety of agricultural products in PAEs-contaminated areas, and could effectively reduce the threat of crop PAEs pollution to human health.

Key words： endophytic flora phthalic acid esters degradation characteristics colonization

Received: 30 August 2023

PACS:

X172

Corresponding Authors: 凌婉婷,教授,lingwanting@njau.edu.cn E-mail: lingwanting@njau.edu.cn

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	ZHANG Shuai
	WANG Jian
	MA Jun-chao
	GAO Yan-zheng
	ZUO Xiang-zhi
	LING Wan-ting

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ZHANG Shuai,WANG Jian,MA Jun-chao等. Screening and performance of phthalate-degrading endophytic floras[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1554-1561.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I3/1554

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