紫外联合过氧甲酸灭活真菌孢子的效能与机制

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Abstract The inactivation efficiency and mechanism of ultraviolet / performic acid (UV/PFA) combined process against two typical pathogenic fungi (Aspergillus Niger and Aspergillus flavus) were investigated in this study. It was found that UV/PFA could inactivate 1.21-log of Aspergillus Niger spores and 2.08-log of Aspergillus flavus spores at 8min, and the inactivation efficiency of combined inactivation was higher than the sum of the inactivation efficiency of PFA and UV alone, showing a synergistic effect. The mechanism of combined inactivation was mainly that UV activated PFA to generate strong oxidizing hydroxyl radicals (·OH) to cause damage to cell membranes, increased the level of intracellular reactive oxygen species to increase cellular stress, and also facilitated the penetration of PFA into the cell interior, thus enhancing the inactivation efficiency. UV played a major role in the combined inactivation process, followed by ·OH, which contributed 87% and 11% to Aspergillus Niger inactivation and 71% and 27% to Aspergillus flavus inactivation, respectively. In addition, after UV/PFA inactivation, the regeneration time of fungi was delayed and the maximum potential of regeneration growth was inhibited.

Key words： fungal spores performic acid ultraviolet disinfection disinfection mechanism regrowth potential

Received: 17 October 2023

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X523

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	Articles by authors
	WANG Jing-yi
	WU Ge-hui
	Lin Wei
	HUANG Ting-lin
	WEN Gang

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WANG Jing-yi,WU Ge-hui,Lin Wei等. Efficacy and mechanism of inactivation of fungal spores by ultraviolet combined with performic acid process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(5): 2440-2448.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I5/2440

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