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| Mechanism of the effect of graphene oxide on nitrogen removal performance of activated sludge |
| YIN Mu-chen1, LI Jun1, WU Yao-dong1, LI Dong-yue1, GAO Peng1, BIAN Xue-ying2, PEI Yan-xue1, LIANG Dong-bo3 |
1. National Engineering Laboratory of Urban Sewage Advanced Treatment and Resource Utilization Technology, Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, China;
2. BGI Engineering Consultants Ltd.. Beijing 100038. China;
3. China Urban Construction Design & Research Institute Co. Ltd., Beijing 100120. China |
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Abstract Three sequencing batch reactors (SBRs) with different concentrations of graphene oxide (GO. 0.1 and 10mg/L) were established to clarify the intrinsic mechanism of GO deteriorating the nitrogen removal performance of activated sludge (AS). The results showed that 1mg/L GO had no significant inhibitory effect on the performance of AS. 10mg/L GO reduced the total nitrogen (TN) removal efficiency of AS by 17.63%. Moreover. GO exposure increased the oxidative stress levels in AS by 198.51% and 307.13%. respectively. The microorganisms responded to the GO stress by secreting more extracellular polymers (EPS). 16S rRNA gene sequencing showed that GO significantly affected the abundance of genera with nitrogen removal function. The abundance of common genera responsible for nitrification (Nitrosomonas and Nitrospira) and denitrification (Candidatus_Accumulibacter. and Thauera. etc.) decreased with increasing GO concentration. Functional gene prediction showed that GO reduced the abundance of genes related to nitrogen removal. The abundance of genes related to NH4+-N oxidation (amoA.
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Received: 28 November 2023
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[1] Kazlauskas M, Jurgelėnė Ž, Aemčuk S, et al. Effect of graphene oxide on the uptake. translocation and toxicity of metal mixture to Lepidium sativum L. plants: Mitigation of metal phytotoxicity due to nanosorption [J]. Chemosphere, 2023,312:137221.
[2] Jiang Y. Quan X. Jiang G. et al. Current Prospective on Environmental Nanotechnology Research in China [J]. Environmental Science & Technology. 2019,53(8):4001-4002.
[3] Sun T Y, Bornhöft N A, Hungerbühler K, et al. Dynamic probabilistic modeling of environmental emissions of engineered nanomaterials [J]. Environmental Science & Technology, 2016,50(9):4701-4711.
[4] Chung C, Kim Y, Shin D, et al. Biomedical applications of graphene and graphene oxide [J]. Accounts of Chemical Research, 2013,46(10): 2211-2224.
[5] Alqadami A A, Naushad M, Abdalla M A, et al. Efficient removal of toxic metal ions from wastewater using a recyclable nanocomposite: A study of adsorption parameters and interaction mechanism [J]. Journal of Cleaner Production, 2017,156:426-436.
[6] Chen X. Chen B. Facile fabrication of crumpled graphene oxide nanosheets and its Platinum nanohybrids for high efficient catalytic activity [J]. Environmental Pollution, 2018,243:1810-1817.
[7] Zhou Q. Hu X. Systemic Stress and Recovery Patterns of Rice Roots in Response to Graphene Oxide Nanosheets[J]. Environmental Science & Technology. 2017,51(4):2022-2030.
[8] Audira G. Lee J S. Siregar P. et al. Comparison of the chronic toxicities of graphene and graphene oxide toward adult zebrafish by using biochemical and phenomic approaches [J]. Environmental Pollution. 2021,278:116907.
[9] Zhao J. Lin M. Wang Z. et al. Engineered nanomaterials in the environment: Are they safe? [J]. Critical reviews in environmental science and technology. 2021,51(14):1443-1478.
[10] Ahmed F. Rodrigues D F. Investigation of acute effects of graphene oxide on wastewater microbial community: A case study [J]. Journal of Hazardous Materials, 2013,256-257:33-39.
[11] Zhou N. Zhao Z. Wang H. et al. The effects of graphene oxide on nitrification and N2O emission: Dose and exposure time dependent [J]. Environmental Pollution, 2019,252:960-966.
[12] Liao Y. Li S. Zhu X. et al. The promotion and inhibition effect of graphene oxide on the process of microbial denitrification at low temperature [J]. Bioresource Technology, 2021,340:125636.
[13] Kong Q. He X. Feng Y. et al. Pollutant removal and microorganism evolution of activated sludge under ofloxacin selection pressure [J]. Bioresour Technol, 2017,241:849-856.
[14] Wang L, Li Y, Wang L, et al. Responses of biofilm microorganisms from moving bed biofilm reactor to antibiotics exposure: Protective role of extracellular polymeric substances [J]. Bioresource Technology, 2018,254:268-277.
[15] Sheng G, Yu H, Li X. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review [J]. Biotechnology advances, 2010,28(6):882-894.
[16] Xu J, Sheng G, Ma Y, et al. Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system [J]. Water Research, 2013,47(14):5298-5306.
[17] 郑林雪.李军.胡家玮.等.同步硝化反硝化系统中反硝化细菌多样性研究[J]. 中国环境科学, 2015,35(1):116-121. Zheng L, Li J, Hu J, et al. Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems [J]. China Environmental Science, 2015,35(1):116-121.
[18] 卞伟.李军.赵白航,等.硝化污泥中AOB/NOB对硝化特性的影响[J]. 中国环境科学, 2016,36(8):2395-2401. Bian W, Li J, Zhao B, et al. The effect of AOB/NOB in nitrifying sludge on nitrification characteristics [J]. China Environmental Science, 2016,36(8):2395-2401.
[19] 张凯.李军.梁东博.等.IFAS工艺短程硝化过程中功能菌的动力学特性[J]. 中国环境科学, 2020,40(4):1507-1514. Zhang K. Li J. Liang D. et al. Kinetic characteristics of functional bacteria in IFAS process for partial nitrification [J]. China Environmental Science, 2023,43(7):3438-3446.
[20] 李爽.李晓敏.李芳柏.Fe(II)对反硝化过程及其功能微生物群落的影响[J]. 中国环境科学, 2018,38(1):263-274. Li S, Li X, Li F. Effect of Fe (II) on denitrification and associated functional microbial communities [J]. China Environmental Science, 2018,38(1):263-274.
[21] Bian X, Wu Y, Li J, et al. Effect of dissolved oxygen on high C/N wastewater treatment in moving bed biofilm reactors based on heterotrophic nitrification and aerobic denitrification: Nitrogen removal performance and potential mechanisms [J]. Bioresource Technology, 2022,365:128147.
[22] Ye J, Gao H, Wu J, et al. Responses of nitrogen transformation processes and N2O emissions in biological nitrogen removal system to short-term ZnO nanoparticle stress [J]. Science of The Total Environment, 2020,705:135916.
[23] Ye J, Gao H, Wu J, et al. Transient disturbance of CeO2 nanoparticles enhances N2O emissions during biological wastewater treatment [J]. Biochemical Engineering Journal, 2022,187:108620.
[24] 朱绒霞,高国棉,赵建峰.等.低温氧化石墨制备技术研究[J]. 应用化工, 2019,48(3):514-516. Zhu R, Gao G, Zhao J, et al. Study on low temperature preparation of graphite oxide [J]. Applied Chemical Industry, 2019,48(3):514-516.
[25] 叶金宇.污水生物脱氮系统对金属纳米颗粒的胁迫响应[D]. 南京:东南大学, 2021. Ye J. Responses of biological nitrogen removal system to metallic nanoparticle stress and regulation mechanism of damaged system [D]. Nanjing: Southeast University, 2021.
[26] Ma W, Zhang J, Wang Y, et al. Extracellular polymeric substances excreted by anammox sludge act as a barrier for As(III) invasion: Binding property and interaction mechanism [J]. Chemosphere, 2021, 278:130414.
[27] Karim M R. Salam K A. Hossain E. et al. Interaction between chronic arsenic exposure via drinking water and plasma lactate dehydrogenase activity [J]. Science of The Total Environment, 2010,409(2):278-283.
[28] Zhang H, Song S, Jia Y, et al. Stress-responses of activated sludge and anaerobic sulfate-reducing bacteria sludge under long-term ciprofloxacin exposure [J]. Water research, 2019,164:114964.
[29] Ye J, Gao H, Wu J, et al. Effects of ZnO nanoparticles on flocculation and sedimentation of activated sludge in wastewater treatment process [J]. Environmental research, 2021,192:110256.
[30] Liang D, Guo W, Li D, et al. Enhanced aerobic granulation for treating low-strength wastewater in an anaerobic-aerobic-anoxic sequencing batch reactor by selecting slow-growing organisms and adding carriers [J]. Environmental Research, 2022,205:112547.
[31] Wang S, Wang G, Yan P, et al. Non-filamentous sludge bulking induced by exopolysaccharide variation in structure and properties during aerobic granulation [J]. Science of the Total Environment, 2023, 876:162786.
[32] Li D, Guo W, Liang D, et al. Rapid start-up and advanced nutrient removal of simultaneous nitrification. endogenous denitrification and phosphorus removal aerobic granular sequence batch reactor for treating low C/N domestic wastewater [J]. Environmental Research, 2022,212:113464.
[33] Gao J, Wu J, Chen S, et al. Nitrogen removal from pharmaceutical wastewater using simultaneous nitrification-denitrification coupled with sulfur denitrification in full-scale system [J]. Bioresource Technology, 2024,393:130066.
[34] Freitas F, Alves V D, Reis M A M. Advances in bacterial exopolysaccharides: from production to biotechnological applications [J]. Trends in biotechnology, 2011,29(8):388-398.
[35] Shi H, Wang J, Liu S, et al. Potential role of AHL-mediated quorum sensing in inducing non-filamentous sludge bulking under high organic loading [J]. Chemical Engineering Journal, 2023,454:140514.
[36] 欧金梅.杨许.单春苗.等.榔梅果实转录组分析及其柠檬酸生物合成途径关键酶基因结构与功能预测[J]. 中国中药杂志, 2020, 45(19):4606-4616. Ou J, Yang X, Shan C, et al. Transcriptome analysis of "Langmei" fruits and key enzyme genes structure and function prediction involved in citric acid biosynthesis [J]. China Journal of Chinese Materia Medica, 2020,45(19):4606-4616.
[37] Kaila V, Wikstrom M. Architecture of bacterial respiratory chains [J]. Nature Reviews Microbiology, 2021,19(5):319-330.
[38] Li Q, Guo X, Lu Y, et al. Impacts of adding FGDG on the abundance of nitrification and denitrification functional genes during dairy manure and sugarcane pressmud co-composting [J]. Waste Management, 2016,56:63-70.
[39] Yin Y, Yang C, Gu J, et al. Roles of nxrA-like oxidizers and nirS-like reducers in nitrite conversion during swine manure composting [J]. Bioresource Technology, 2020,297:122426.
[40] Chen D, Wang X, Yang K, et al. Response of a three dimensional bio-electrochemical denitrification system to the long-term presence of graphene oxide [J]. Bioresource Technology, 2016,214:24-29.
[41] Kedves A, Sánta L, Balázs M, et al. Chronic responses of aerobic granules to the presence of graphene oxide in sequencing batch reactors [J]. Journal of Hazardous Materials, 2020,389:121905. |
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