高效柴油降解菌的筛选及其对烷烃组分的降解

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Abstract A diesel degrading bacterial strain 13-3isolated from oil-contaminated soil in an oilfield mining area in Northeast China was identified as Acinetobacter sp. by morphological observation and 16S rRNA. The strain could degrade 88.50% diesel of 1g/L cultured at 20℃ at 180r/min for 7days, which showed excellent diesel degrading ability compared with other strains, and the degradation rate of diesel fuel could reach 71.7% after being inoculated into 10g/kg diesel contaminated soil and remediation at 20℃ and soil moisture content of 30% for 21days, which indicated it had great potential in practical diesel-contaminated soil remediation applications. According to the analysis of GC-MS spectrum and the degrading experiments of different chain length alkane substrates, strain 13-3had a wide alkane utilization spectrum (C₁₄~C₂₂), and the degradation rate of C₁₇~C₂₀ could reach more than 90%. The results of environmental factors showed that strain 13-3could grow in the range of pH=6~9, temperature 10~30℃, salinity (NaCl) 0.5%~3%, and still had a high concentration of bacterial solution at the initial diesel concentration of 10g/L, which indicated that strain 13-3 had good environmental adaptability and was expected to exert its degradation performance under complex environmental conditions. Through whole-genome sequencing analysis, three key genes (alkB, almA, ladA) were found in the genome of strain 13-3, and the possible degradation pathway of strain 13-3 was speculated to be the terminal oxidation pathway.

Key words： strain screening and identification Acinetobacter diesel degradation alkane degradation

Received: 22 February 2024

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	CHEN Hong-chu
	ZHANG Ting-di
	FU Yu-feng
	RU Jin-tao
	QIN Chuan-yu

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CHEN Hong-chu,ZHANG Ting-di,FU Yu-feng等. Isolation of a high-efficient diesel degrading bacteria and its degradation of alkane components of diesel[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(10): 5723-5732.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I10/5723

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