武汉市湖泊水体三种致腹泻病原菌的分布特征

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Abstract Field surveys were conducted in Wuhan city and its surrounding areas to assess the water quality of urban and agricultural lakes during winter and summer. Additionally, fluorescence quantitative PCR technology was employed to analyze the distribution patterns of three diarrheal pathogens. The results revealed that the average abundance of Escherichia coli uidA gene [(1.86±1.15)×10⁹ copies/100mL] and the Shigella dysenteriae ipaH gene [(2.20±1.54)×10⁵ copies/100mL] in urban lakes was approximately 2- and 2.7-times higher, respectively, compared to agricultural lakes (P<0.05). However, no significant regional difference was observed in the abundance of Salmonella spp. between urban and agricultural lakes. During the summer, the abundance of the E. coli uidA gene [(3.75±1.48)×10⁹ copies/100mL] in lake water was significantly higher compared to the winter [(1.45±1.07)×10⁹copies/100mL] (P<0.01). Conversely, no significant seasonal difference was observed in the abundance of Salmonella spp. and S. dysenteriae in the lake water. The abundance of E. coli, an indicator of fecal contamination, showed a significant positive correlation with the presence of S. dysenteriae ipaH gene (P<0.0001). However, no significant linear relationship was found between the abundance of the E. coli uidA gene and the presence of the Salmonella spp. dam gene (P>0.05). In the lake water, the abundance of the E. coli uidA gene and the S. dysenteriae ipaH gene showed significant positive correlations with chlorophyll-a, total phosphorus, and ammonium nitrogen. Overall, sewage discharge and surface runoff have led to nutrient enrichment and a significant increase in the abundance of pathogenic microorganisms in urban lakes. In summer, higher levels of suspended particles, predominantly composed of algae, promote the attachment and growth of pathogenic bacteria like E. coli. To accurately reflect pathogenic bacterial contamination in water bodies and control biological risks, it is necessary to increase the variety of pathogenic bacteria monitored in environmental water.

Key words： Escherichia coli urbanization seasonality functional genes chlorophyll-a indicator bacteria diarrhea

Received: 03 March 2024

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X524

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	DENG Min
	WANG Yu-ren
	LIU Wen-jing
	LI Lu
	SONG Kang

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DENG Min,WANG Yu-ren,LIU Wen-jing等. The distribution characteristics of three types of diarrheal pathogens in the lakes of Wuhan city[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(10): 5875-5884.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I10/5875

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