磁铁矿作用下<i>Dv</i>H阴极生物膜的响应及转录组分析

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Abstract This study investigated the response mechanism of the biofilm by constructing a Desulfovibrio vulgaris Hildenborough (DvH) biocathode, under the influence of magnetite nanoparticles (MNPs). The results showed that the conversion rate of SO₄^2- to S^2- of the biocathode mediated by MNPs increased from 6.8% to 37.9%, compared to the group without MNPs. The results of the linear sweep voltammetry test revealed that, within the potential range of 0~-0.81V, the cathodic current observed in the group with MNPs was higher than that of the control group, suggesting that MNPs enhance the electrocatalytic activity of the cathodic biofilm. The comparative transcriptome analysis of DvH cathodic biofilm revealed that MNPs can facilitate hydrogen utilization in DvH by upregulating genes encoding [FeFe] hydrogenase, Hmc, and ATP synthase. In addition, genes encoding sulfate adenylyltransferase and adenylylsulphate reductase, both crucial enzymes in sulfur metabolism, were upregulated with MNPs addition, which contributed to an improved conversion rate of SO₄^2--S^2-. MNPs upregulated genes related to Flp/Tad pili assembly and PEP-CTERM protein, thereby augmenting the adhesion of DvH and promoting biofilm formation on the cathode. These findings contribute to a novel theoretical framework for the development of highly efficient microbial cathodic electrochemical systems.

Key words： microbial electrochemical system (MES) sulfate reducing bacteria biocathode magnetite nanoparticles transcriptomic

Received: 03 April 2024

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X703.1

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	Articles by authors
	TENG Min
	ZHU Xi
	ZENG Cui-ping
	HU Jia-ping
	LIU Guang-li
	LUO Hai-ping

Cite this article:

TENG Min,ZHU Xi,ZENG Cui-ping等. Response and transcriptional analysis of DvH cathode biofilm with the effect of magnetite[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6088-6095.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I11/6088

[1] Khanal S K, Huang J C. ORP-based oxygenation for sulfide control in anaerobic treatment of high-sulfate wastewater [J]. Water Research, 2003,37(9):2053-2062.
[2] 相凤欣,任立人,吴丹,等.生物处理含硫酸盐废水生成单质硫的研究进展 [J]. 环境工程, 2012,30(S2):151-155. Xiang F X, Ren L R, Wu D, et al. Advances in bio-treatment of sulfate-laden wastewater and conversion to element sulfur [J]. Environmental Engineering, 2012,30(S2):151-155.
[3] 焦玲洁,李咏梅,魏海娟,等.铁及其化合物控制污泥厌氧消化VSCs机理研究进展 [J]. 中国环境科学, 2023,43(7):3454-3463. Jiao L J, Li Y M, Wei H J, et al. A review on the mechanism of adding iron and iron compounds to control volatile sulfur compounds during anaerobic digestion of sludge [J]. China Environmental Science, 2023, 43(7):3454-3463.
[4] Shi K, Cheng W, Jiang Q, et al. Insight of the bio-cathode biofilm construction in microbial electrolysis cell dealing with sulfate-containing wastewater [J]. Bioresource Technology, 2022,361(19): 127695.
[5] Logan B E, Call D, Cheng S, et al. Microbial electrolysis cells for high yield hydrogen gas production from organic matter [J]. Environmental Science & Technology, 2008,42(23):8630-8640.
[6] 卢建宏,李卓,孙驰贺,等.耦合发酵产氢尾液处理的微生物电化学系统研究 [J]. 中国环境科学, 2019,39(10):4157-4163. Lu J H, Li Z, Sun C H, et al. Degradation of dark fermentation effluents for biogas production using bioelectrochemical systems [J]. China Environmental Science, 2019,39(10):4157-4163.
[7] 周美洲,骆海萍,曾翠平,等.微生物光电还原CO₂合成乙酸对外电压的响应机制 [J]. 中国环境科学, 2022,42(2):907-913. Zhou M Z, Luo H P, ZENG C P, et al. Microbial photoelectric reduction of CO₂ to acetate and its response mechanism to external applied voltage [J]. China Environmental Science, 2022,42(2):907-913.
[8] 赵婷,冯雍,谢倍珍,等.种源及培养方式对反硝化生物阴极性能的影响 [J]. 中国环境科学, 2023,43(7):3489-3498. Zhao T, Feng Y, Xie B Z et al. Effect of inoculum and cultivation mode on the performances of denitrifying biocathodes [J]. China Environmental Science, 2023,43(7):3489-3498.
[9] Pant D, Singh A, Van Bogaert G, et al. Bioelectrochemical systems (BES) for sustainable energy production and product recovery from organic wastes and industrial wastewaters [J]. Rsc Advances, 2012, 2(4):1248-1263.
[10] Luo H, Bai J, He J, et al. Sulfate reduction and elemental sulfur recovery using photoelectric microbial electrolysis cell [J]. Science of the Total Environment, 2020,728(31):138685.
[11] Teng W, Liu G, Luo H, et al. Simultaneous sulfate and zinc removal from acid wastewater using an acidophilic and autotrophic biocathode [J]. Journal of Hazardous Materials, 2016,304(4):159-165.
[12] Aulenta F, Fazi S, Majone M, et al. Electrically conductive magnetite particles enhance the kinetics and steer the composition of anaerobic TCE-dechlorinating cultures [J]. Process Biochemistry, 2014,49(12): 2235-2240.
[13] Liu F, Rotaru A E, Shrestha P M, et al. Magnetite compensates for the lack of a pilin-associated c-type cytochrome in extracellular electron exchange [J]. Environmental Microbiology, 2015,17(3):648-655.
[14] Kato S, Hashimoto K, Watanabe K. Microbial interspecies electron transfer via electric currents through conductive minerals [J]. Proceedings of the National Academy of Sciences of the United States of America, 2012,109(25):10042-10046.
[15] Viggi C C, Rossetti S, Fazi S, et al. Magnetite particles triggering a faster and more robust syntrophic pathway of methanogenic propionate degradation [J]. Environmental Science & Technology, 2014,48(13):7536-7543.
[16] Hu J, Zeng C, Liu G, et al. Magnetite nanoparticles accelerate the autotrophic sulfate reduction in biocathode microbial electrolysis cells [J]. Biochemical Engineering Journal, 2018,133(5):96-105.
[17] Heidelberg J F, Seshadri R, Haveman S A, et al. The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough [J]. Nature Biotechnology, 2004,22(5):554-559.
[18] Cruz V C, Rossetti S, Fazi S, et al. Magnetite particles triggering a faster and more robust syntrophic pathway of methanogenic propionate degradation [J]. Environmental Science & Technology, 2014,48(13):7536-7543.
[19] Luo H, Teng W, Liu G, et al. Sulfate reduction and microbial community of autotrophic biocathode in response to acidity [J]. Process Biochemistry, 2017,54(3):120-127.
[20] Luo H, Fu S, Liu G, et al. Autotrophic biocathode for high efficient sulfate reduction in microbial electrolysis cells [J]. Bioresource Technology, 2014,167(17):462-468.
[21] Blazquez E, Gabriel D, Baeza J A, et al. Evaluation of key parameters on simultaneous sulfate reduction and sulfide oxidation in an autotrophic biocathode [J]. Water Research, 2017,123(16):301-310.
[22] Fu Z, Yan L, Li K, et al. The performance and mechanism of modified activated carbon air cathode by non-stoichiometric nano Fe₃O₄ in the microbial fuel cell [J]. Biosensors & Bioelectronics, 2015,74(12): 989-995.
[23] Yu H, Yan X, Weng W, et al. Extracellular proteins of Desulfovibrio vulgaris as adsorbents and redox shuttles promote biomineralization of antimony [J]. Journal of Hazardous Materials, 2022,426(6):127795.
[24] Murugan M, Miran W, Masuda T, et al. Biosynthesized iron sulfide nanocluster enhanced anodic current generation by sulfate reducing bacteria in microbial fuel cells [J]. Chemelectrochem, 2018,5(24): 4015-4020.
[25] Zhou Y, Gao Y, Xie Q, et al. Reduction and transformation of nanomagnetite and nanomaghemite by a sulfate-reducing bacterium [J]. Geochimica Et Cosmochimica Acta, 2019,256(13):66-81.
[26] 周轶臣,杨慧芬,宋英良,等.硫酸盐还原菌作用下铁矾渣的还原溶解和相转化 [J]. 中国环境科学, 2023,43(S1):170-178. Zhou Y C, Yang H F, Song Y L, et al Reductive dissolution and phase transformation of jarosite residues by SRB [J]. China Environmental Science, 2023,43(S1):170-178.
[27] Deng X, Dohmae N, Kaksonen A H, et al. Biogenic iron sulfide nanoparticles to enable extracellular electron uptake in sulfate-reducing bacteria [J]. Angewandte Chemie-International Edition, 2020, 59(15):5995-5999.
[28] Xiao Y, Zhang E, Zhang J, et al. Extracellular polymeric substances are transient media for microbial extracellular electron transfer [J]. Science Advances, 2017,3(7):e1700623.
[29] Wang C, Wang C, Jin L, et al. Response of syntrophic aggregates to the magnetite loss in continuous anaerobic bioreactor [J]. Water Research, 2019,164(17):114925.
[30] Voordouw G, Brenner S. Nucleotide sequence of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough) [J]. Eur J Biochem, 1985,148(3):515-520.
[31] Posewitz M C, King P W, Smolinski S L, et al. Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase [J]. Journal of Biological Chemistry, 2004,279(24):25711-25720.
[32] Mansure J J, Hallenbeck P C. Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase [J]. Biotechnology Letters, 2008,30(10):1765-1769.
[33] Caffrey S M, Park H S, Voordouw J K, et al. Function of periplasmic hydrogenases in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough [J]. Journal of Bacteriology, 2007,189(17):6159-6167.
[34] Fauque G, Peck H J, Moura J J, et al. The three classes of hydrogenases from sulfate-reducing bacteria of the genus Desulfovibrio [J]. Fems Microbiology Reviews, 1988,4(4):299-344.
[35] Mohanraj S, Kodhaiyolii S, Rengasamy M, et al. Phytosynthesized iron oxide nanoparticles and ferrous iron on fermentative hydrogen production using Enterobacter cloacae : Evaluation and comparison of the effects [J]. International Journal of Hydrogen Energy, 2014,39(23): 11920-11929.
[36] Wang J, Wan W. Effect of Fe²⁺ concentration on fermentative hydrogen production by mixed cultures [J]. International Journal of Hydrogen Energy, 2008,33(4):1215-1220.
[37] Bryant R D, Van Ommen K F, Laishley E J. Regulation of the periplasmic [Fe] hydrogenase by ferrous iron in Desulfovibrio vulgaris (Hildenborough) [J]. Applied and Environmental Microbiology, 1993,59(2):491-495.
[38] Peters J W, Schut G J, Boyd E S, et al. [FeFe]-and [NiFe]-hydrogenase diversity, mechanism, and maturation[J]. Biochimica Et Biophysica Acta-Molecular Cell Research, 2015,1853(6):1350-1369.
[39] Li Y, Zhao Z, Yu Q, et al. High-efficiency ethanol yield from anaerobic fermentation of organic wastes via stimulating growth of ethanol-producing Fe (Ⅲ)-reducing bacteria with magnetite [J]. Acs Sustainable Chemistry & Engineering, 2021,9(3):1246-1253.
[40] Dolla A, Pohorelic B, Voordouw J K, et al. Deletion of the hmc operon of Desulfovibrio vulgaris subsp vulgaris Hildenborough hampers hydrogen metabolism and low-redox-potential niche establishment [J]. Archives of Microbiology, 2000,174(3):143-151.
[41] Franco L C, Steinbeisser S, Zane G M, et al. Cr (VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris [J]. Applied Microbiology and Biotechnology, 2018,102(6): 2839-2850.
[42] Sim M S, Ogata H, Lubitz W, et al. Role of APS reductase in biogeochemical sulfur isotope fractionation [J]. Nature Communications, 2019,10(1):44.
[43] Zhou A, Baidoo E, He Z, et al. Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution [J]. Isme Journal, 2013,7(9):1790-1802.
[44] McCallum M, Burrows L L, Howell P L. The dynamic structures of the type IV pilus [J]. Microbiology Spectrum, 2019,7(2):113-128.
[45] Gao N, Xia M, Dai J, et al. Both widespread PEP-CTERM proteins and exopolysaccharides are required for floc formation of Zoogloea resiniphila and other activated sludge bacteria [J]. Environmental Microbiology, 2018,20(5):1677-1692.