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| Effects of complex carbon sources on phosphorus enrichment and microbial communities in biofilm systems |
| BI Zhen1, FU Hao1, WANG Xue-ling1,2, YUAN Yi-na1,2, HUANG Yong1,2 |
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China |
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Abstract In this study, the effects of complex carbon sources on the performance of phosphorus enrichment and characteristics of microbial community were investigated in an anaerobic/aerobic alternatively operational biofilm reactor by using synthetic sewage wastewater containing VFAs, glucose and amino acid. The results showed that the utilization efficiency of complex carbon source reached 72.5% by adjusting the parameters of anaerobic hydraulic retention time, aerobic pH and DO concentration. The removal efficiency of total phosphorus and total nitrogen were 99.8% and 76.5%, respectively, with the concentration of phosphorus enrichment solution exceeding 50mg/L. The utilization complex carbon source changed the energy metabolism pathway of biofilm. Specifically, amino acids may participate in phosphorus metabolism as intracellular energy storage substances to compensate for the energy deficiency caused by PHA and glycogen, thus maintaining a good phosphorus removal and enrichment performance of the biofilm system. Meanwhile, the content of intracellular organophosphorus (OP) fluctuated with the activity of phosphorus uptake and release, whereas the content of inorganic poly-phosphorus (IP) changed a little. This phenomenon indicated that the phosphorus metabolism transferred from the IP pathway to the OP pathway. The abundance of hydrolytic bacteria and fermentative bacterium in biofilm increased significantly, which could provide small molecular organic carbon for phosphorus-accumulating bacteria (PAOs) metabolism, thus contribute the satisfactory performance of phosphorus removal and enrichment. The conclusion of present study exhibited the potential of biofilm system to simultaneously realize the phosphorus enrichment and total nitrogen removal via using complex carbon sources in sewage wastewater.
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Received: 12 April 2024
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