Nano-Fe<sub>3</sub>O<sub>4</sub>强化牛粪厌氧发酵及抗性基因削减

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Abstract In order to improve the biogas production performance of high-concentration anaerobic fermentation of cow manure, the enhancement effect of different concentrations of nano iron trioxide (Nano-Fe₃O₄) on substrate hydrolysis and biogas production efficiency was tested, and the influence on microbial community structure and antibiotic resistance genes (ARGs) in the fermentation system were further explored. The results showed that adding 500mg/L of Nano-Fe₃O₄ can improve the hydrolysis degree of high concentration anaerobic fermentation substrates in cow manure, enhance system stability, and increase biogas production by 12.36%. Compared with the control, the abundance of Firmicutes and Fibrobacters in the fermentation system significantly increased by 37.39% and 75.86%, respectively. At the genus level, especially the abundance of Clostridium and Ruminococcus was 3.50 times and 8.69 times higher than that before. Methanosarcina was dominant among methanogens,with an abundance of 2.55%, a significant increase of 27.50% over the control. In addition, the abundance of potential pathogenic bacteria Treponema and Acinetobacter was significantly decreased by 72.14% and 93.69%, respectively. The reduction rate of tetracyclines (tetA,tetG,tetH,tetJ) and aminoglycosides (aph(3), aph(6)) in ARGs was 100%; The average reduction rate of sulfonamide class (sul1, sul2) was 74.41%~96.55%. Research has shown that adding an appropriate amount of Nano-Fe₃O₄ had multiple promoting effects on stabilizing the fermentation system, improving microbial activity, increasing biogas production, and reducing antibiotic resistance genes, etc. The research results can provide relevant theoretical guidance and practical basis for the use of conductive exogenous regulatory additives such as Nano-Fe₃O₄ in livestock and poultry manure biogas projects.

Key words： Nano-Fe₃O₄ cow manure high concentration anaerobic fermentation antibiotic resistance genes

Received: 22 March 2024

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X712

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	Articles by authors
	XI Yan-hua
	WANG Xin-zhi
	LI Xu
	WEI Shuai-qiang
	SUN Li-bo
	LYU Ya-tian
	CHENG Hui-cai

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XI Yan-hua,WANG Xin-zhi,LI Xu等. Enhancement of high concentration anaerobic fermentation of cow dung and reduction of antibiotic resistance genes by Nano-Fe₃O₄[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6250-6259.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I11/6250

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