氨氧化微生物对硝化潜势和N<sub>2</sub>O生成的贡献

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Abstract This study utilized reverse transcription quantitative real-time PCR to investigate the spatial distribution of amoA gene expression levels among ammonia-oxidizing microorganisms in the surface sediments of the East China Sea during spring. Cultivation experiments were performed to assess the relative contributions of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) to the nitrification potential and N₂O production in the surface sediments. The results revealed that the expression level of AOA amoA gene was significantly higher than that of AOB (AOA: 4.49×10²~2.17×10⁶copies/g;AOB: 6.60×10¹~7.65×10⁵copies/g), with both showing a spatial distribution pattern of lower levels in nearshore areas (AOA: 8.92×10⁵copies/g; AOB: 2.06×10³copies/g) and higher in offshore areas (AOA: 1.05×10⁶copies/g; AOB: 4.06×10⁴copies/g). Nitrification potential was found to be higher in nearshore sediments than in offshore sediments, with the nitrification process gradually shifting from AOA dominance in nearshore areas to AOB dominance in offshore areas. In the N₂O production process, the addition of NH₄⁺ significantly enhanced N₂O production, with AOB playing a more critical role than AOA in this process.

Key words： East China Sea sediment ammonia-oxidizing microorganisms potential nitrification rate N₂O

Received: 26 May 2024

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	WANG Da-ling
	YANG Yu-hong
	HE Hui
	MI Tie-zhu
	ZHEN Yu

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WANG Da-ling,YANG Yu-hong,HE Hui等. Contribution of ammonia-oxidizing microorganisms to nitrification potential and N₂O production[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(12): 6828-6837.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I12/6828

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