A<sup>2</sup>O污水生物处理过程中DOM对N<sub>2</sub>O生成的影响

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Abstract In this study, the components of dissolved organic matter (DOM) during the anaerobic-anoxic-aerobic (A²O) biological wastewater treatment process was analyzed by using fluorescence emission excitation matrix combined with parallel factor analysis (3D EEMs-PARAFAC), and the generation of nitrous oxide (N₂O) in each unit was also quantified. Additionally, machine learning model was employed to further predict the response relationship between DOM components and N₂O generation. Results showed that DOM in the influent of the wastewater treatment plants (WWTP) was primarily composed of four components, including tryptophan (C1), fulvic acid (C2), humic acid (C3), and tyrosine (C4), while C1 and C4 being the dominant components. The concentration of DOM decreased progressively throughout the treatment process, while the removal efficiency of readily biodegradable DOM (such as C1and C4) were significantly higher than that of C2 and C3. N₂O emission was the major component of direct carbon emissions and showed significant spatial heterogeneity. The N₂O emission amount of each unit ranked from high to low were observed in the following order: oxic tank, secondary sedimentation tank, anoxic tank, anaerobic tank, grille, and primary sedimentation tank. Shapley Additive exPlanation (SHAP) analysis revealed that C1 and C2 would significantly affect the N₂O generation process, while the effects of C3 and C4 were negligible. Specifically, C1would enhance N₂O generation, while C2 had an adverse effect. High-throughput sequencing results indicated that Methylotenera and Terrimonas, which could utilize readily biodegradable organic matter for denitrification, were the dominant bacterial genera in the sludge of WWTP. Overall, this study revealed disparate response between N₂O generation and different DOM components during the A²O process, which would help to improve the current carbon emission accounting method of WWTPs and provide theoretical support for optimizing their low-carbon operation processes.

Key words： wastewater treatment plant (WWTP) nitrous oxide (N₂O) dissolved organic matter (DOM) anaerobic-anoxic-aerobic (A²O) carbon emission

Received: 23 October 2024

PACS:

X703

Corresponding Authors: 胡振,责任作者,教授,huzhen885@sdu.edu.cn E-mail: huzhen885@sdu.edu.cn

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	JIANG Hui-qi
	YU Pei-han
	HU Zhen
	REN Yan-gang
	HAO Ze-yu
	HAN Ke
	XUE Chen-yang
	WANG Jin-he

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JIANG Hui-qi,YU Pei-han,HU Zhen等. Effect of dissolved organic matter on N₂O generation in A²O biological wastewater treatment process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 1869-1877.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/1869

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