蚯蚓肠道铁还原过程对活性氧形成的影响

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Abstract Earthworm (Pheretima guillelmi) was selected as the model animal. Combination of in situ determination, high-throughput sequencing and biochemical analysis, the results indicated that the production of Fe(Ⅱ) was highest in the glucose treatment group and lowest in the amino acid setup. During the iron(Ⅲ) reduction process, the content of surface-adsorbed Fe(Ⅱ) was the highest, ranging from 0.6 to 24.38mmol/L;whereas the content of ionic Fe(Ⅱ) was the lowest, ranging from 0.02 to 2.21mmol/L. The community structure of iron(Ⅲ)-reducing bacteria was significantly influenced by the type of organic matter,and the dominant iron(Ⅲ)-reducing bacteria in different treatment groups were diverse. Additionally, the iron(Ⅲ) reduction process was accompanied by the generation of reactive oxygen species (ROS). The content of hydrogen peroxide (H₂O₂) was the highest, ranging from 0.32 to 0.73mmol/L, and be show a significant positive correlation with the contents of ionic Fe²⁺, surface-adsorbed Fe(Ⅱ), and high-crystalline iron, while exhibiting a significant negative correlation with the content of iron in organic complex state. Hydroxyl radical (^•OH) has a significant positive correlation with ionic Fe²⁺, adsorbed Fe(Ⅱ), and high-crystalline iron. Superoxide anion (O₂^•−) has a significant positive correlation with low-crystalline iron and a significant negative correlation with adsorbed Fe(Ⅱ) and high-crystalline iron. The research results provide a new perspective for understanding the role of earthworms gut microbiota in soil iron cycling and ROS formation, which can be applied for pollutant control and degradation.

Key words： gut microbiota of earthworm iron(Ⅲ) reduction organic matter reactive oxygen species

Received: 15 October 2024

PACS:

X172

Corresponding Authors: 周国伟,责任作者,副教授,gwzhou@ahu.edu.cn E-mail: gwzhou@ahu.edu.cn

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	ZHANG Ying
	GAO Ze-ping
	WANG Xu
	LI Shun-shun
	HUANG Tao
	ZHOU Guo-wei

Cite this article:

ZHANG Ying,GAO Ze-ping,WANG Xu等. Effect of intestinal iron reduction process on reactive oxygen species formation in earthworm[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 2053-2062.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/2053

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