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Current Issue
2009 Vol.29 Issue.12,
Published 2009-12-20
1233
Reason analysis of the dust aerosols’ content change in desert regions.
DENG Zu-Qin, HAN Yong-Xiang, BAI Hu-Zhi, ZHAO Tian-Liang
Analysis of the content of dust aerosols in desert regions based on the TOMS aerosol index (AI) as well as solar radiation、dust storm frequency and precipitation was deducted. The AI of desert regions depended not only on dust storms but also on solar radiation. Content of dust aerosols in desert regions were based on the combined effect of sand model and dust devil- convective plume model, and the latter might be more important.
2009 Vol. 29 (12): 1233-1238 [
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1239
Metabolic characteristics of carbon sources of three strains by formaldehyde-degrading fungi.
HUANG Sai-Hua, CHEN Neng-Chang, ZHENG Yu-Ji, XU Sheng-Guang, LIU Ting-Lin
The formaldehyde-degradation characteristics of three strains of formaldehyde-degrading fungi (Trichoderma viride H1, Penicillium javanicum H2, Aspergillus flavus H4) in different carbon sources media was studied. Four kinds of complex carbon sources (0.1% formaldehyde with 10% sucrose, 10% maltose, 10% starch, and 10% glucose, respectively) could be used by the three strains of formaldehyde-degrading fungi and the formaldehyde degradation rate were arrived at 99.3%, 70.1% and 99.7%, respectively. The results of using formaldehyde as the sole carbon source showed that the formaldehyde degradation rate were 88.4%, 64.1% and 63.0%, respectively. The growth of the mycelium of H1 was obtained.
2009 Vol. 29 (12): 1239-1242 [
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1243
Modelling research on the aerosol scattering hygroscopic growth factor based on measurement—Taking 2006 CAREBeijing campaign for example.
LIU Xin-Gang, ZHANG Yuan-Hang
As a part of CAREBeijing campaign, water-soluble ion mass concentration of aerosols were measured with particle-into-liquid sampler as well as the size distribution of water-soluble ion, particulate organic matter and elemental carbon measured by the MOUDI sampler. Based on these measurements, the existence patterns of each chemical species were assumed and reconstructed and then their mass concentration and number size distribution were calculated. Based on the measurements of size-resolved chemistry, relative humidity (RH), and published functional relationships between the chemical composition and water uptake, the aerosol scattering coefficients could be calculated by the Mie theory for the major particle species. The aerosol scattering hygroscopic growth factor f (RH) was then calculated. Closure study could be achieved and results from the closure study showed that the measured and the modeled values of f (RH) agree within measurement uncertainties.
2009 Vol. 29 (12): 1243-1248 [
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1249
Developing carbon resource with two-stage complete mixing ferment process.
REN Jian, LI Jun, ZHOU Jun- Zhang-Shuai, GAN Yi-Ping
Two-stage complete mixing ferment process was used for developing carbon source. Influence of HRT and SRT on the ferment system were studied. Effective HRT of the process was between 32 to 36 h, when SRT was 4 d and sludge recycle rate was 1, average SCOD of effluents was in the range of 1090~1180 mg/L, while separation effect of the sludge thickener was very well, and MLSS of supernatant was in the range of 50~80 mg/L; In the condition of HRT was 32 h and sludge recycle rate was 1, effective SRT of the process was in the range of 4~7d, and average SCOD of effluents was between 980~1179 mg/L, acidogenic bacteria and methanogenic bacteria could be separated effectively. Alkalinity was an important parameter of the primary sludge ferment system. In the process of primary sludge hydrolysis and acidification, ammonia nitrogen and phosphorus would be released. In the conditions of HRT was 32 h and SRT was 4 d, the release rate of ammonia nitrogen and phosphorus were 3.88 mgN/gVSS and 0.27 mgP/gVSS.
2009 Vol. 29 (12): 1249-1254 [
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1255
Enhanced biodegradability of wastewater with electrochemical oxidation on boron-doped diamond anode.
PANG Ya-Ning, ZHAO Guo-Hua, LIU Lei, GAO Jun-Xia
Electrochemical oxidation on boron-doped diamond (BDD) anode was used as pretreatment for 2-chlorophenol wastewater to improve its biodegradability, using DSA electrode as a comparison. And the mechanism was also discussed. Afeer 2h electrochemical oxidation of 2-chlorophenol wastewater on BDD electrode, BOD5/CODCr reached 0.42, while the value only reached 0.24 on DSA electrode. This was closely related with the properties of the electrode. BDD electrode had a high oxygen evolution potential, and many hydroxyl radicals were generated in the solution. This led to that 2-chlorophenol was fully ring-opening, forming intermediates such as oxalic and maleic acids which were easy to be biodegraded. Thus, the biodegradability of 2-chlorophenol wastewater was enhanced greatly. However, DSA electrode had lower oxygen evolution potential and poorer ability of generating of hydroxyl radicals. So the biodegradability of wastewater could not be improved effectively. Therefore, electrochemical oxidation on BDD electrode was an effective approach to improve the biodegradability of organic pollutants.
2009 Vol. 29 (12): 1255-1259 [
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1260
Application of biological iron sulfide composites in chromium-containing wastewater treatment and chromium reclamation.
The resistance to Cr(VI) and regeneration character of biological iron sulfide composites were studied. The composites consisted of sulfate reducing bacteria(SRB) and nanosized iron sulfide produced by SRB. A new reduction-regeneration recirculation process for treating chromium-containing wastewater and reclaiming chromium was developed with the regeneration character of the composites. The SRB still survived after treating wastewater containing Cr(VI), which could regenerate biological iron sulfide composites by using Fe3+, sulphur as electron acceptor. Furthermore, the SRB could also resist 600mg/L Cr(VI) and remove it gradually. The results of reduction-regeneration recirculation process for treating wastewater indicated that the contaminants in the effluent could meet the discharge standard, i.e. the concentration of total chromium less than 0.929mg/L, Cr(VI) less than 0.019 mg/L. After 10 cycles, the Cr2O3 content in sludge reached 40.47%, and the ratio of Cr2O3/FeO was 6.98. The sludge reached the chemical and metallurgical(hydrometallurgy) grade of chromite ore, which could be reclaimed again.
2009 Vol. 29 (12): 1260-1265 [
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1266
Decolorization of azo dye reactive brilliant red K-2BP by a novel yeast Issatchenkia orientalis YP-1.
ZHANG Mu-Ming, YU Zhi-Sheng, ZHANG Hong-Xun, ZHANG Ling
An yeast strain YP-1 was isolated on selective media containing azo dye reactive brilliant red K-2BP, and identified as Issatchenkia orientalis. The strain YP-1 could effectively decolorize reactive brilliant red K-2BP within the concentration of 400mg/L. It gave a maximal decolorization of 99% for reactive brilliant red K-2BP of 100mg/L after incubation for 12h, under the optimal culture conditions: inoculation volume 10%(V/V), pH within the range from 3 to 9, concentration of (NH4)2SO4 30.02%, and concentration of glucose 30.2%. The strain YP-1 could absorb reactive brilliant red K-2BP and subsequently biodegrade it. The strain YP-1 could also decolorize another azo dye reactive black 5 of 200mg/L, up to the decolorization of 99.5%.
2009 Vol. 29 (12): 1266-1271 [
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1272
Triphenylmethane dyes decolorization enzyme over expression and purification in E. coli.
CHEN Liang, REN Sui-Zhou, ZHANG Pei-Pei, XU Mei-Ying, SUN Guo-Ping
The gene coding triphenylmethane dyes degradation enzyme (TpmD) was amplified from genomic DNA of Aeromonas hydrophila strain DN322 by PCR and cloned into the expression vector pET22b (+). After being confirmed by sequencing, the recombinant vector pET22-tpmD was transformed into Escherichia coli BL21(DE3), and then a clone with high yeild of TpmD was screened. The expression of TpmD was induced with IPTG and the specific activity of TpmD from pET22-tpmD/BL21 (DE3)’s cell-free extract, were 569.5,386.9,516.1 and 273.0U/g with crystal violet, malachite green, fuchsin basic and brilliant green as substrates, respectively. After one step purification by Ni-NTA agarose column, the specific activities of TpmD were increased to 1075.3, 1042.8, 903.9 and 484.3U/g, the purity of this enzyme reached up to 94.05%. The stability of the recombinant plasmid makes it easy to produce the protein on a large scale fermention.
2009 Vol. 29 (12): 1272-1276 [
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1277
Diversity of bacterial community in the sediments of the Northern Yellow Sea.
BAI Jie, LI Hai-Yan, ZHANG Jian, ZHAO Yang-Guo
To reveal the diversity and difference of marine bacteria in the sediment of the Northern Yellow Sea, the 16S rDNA clone library technique was used to analyze the characteristics of bacterial community at different seasons from the five stations of the Northern Yellow Sea. The 16S rDNA clone library analysis indicated that the sediments contained high diverse of bacteria and mainly distributed in five to ten known phyla, and most of sequences were affiliated to uncultured bacteria. Phylogenetic analysis showed that the Proteobacteria was the dominant group in the sediments, accounting for 46%~60% of total sequences, and γ-Proteobacteria and δ-Proteobacteria were dominant classes. However, all proteobacterial classes presented phylogenetical divergence at the five stations. The bacterial community diversity differed due to the geographical location and season, and different function microbial groups were closely related to their special habitats.
2009 Vol. 29 (12): 1277-1284 [
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1285
Investigation on germination of phytoplankton resting cells in sediment traps collected from Daya Bay, South China Sea.
KANG Wei, WANG Chao-Hui, FU Yong-Hu, CAO Yu, ZHANG Yu-Juan
In order to understand the effect of cyst beds on phytoplankton population dynamics and redtide occurrence, sediments were collected from two stations in the aquacultural areas of Daya Bay, South China Sea from April 2005 to June 2006. Sediments were filtered through 20 and 125 μm of seive, and incubated at 25℃ for 20 d and 40 d. A total of 37 phytoplankton species, belonging to 27 genera, were obtained from germination incubation. Gymnodinium corii Schiller, a first recorded species from the China Sea, was the dominant dinoflagellate species, average accounting for 73.1% of the proliferating dinoflagellate cells, but the highest proportion could reach 99.7%. Haptophyta species Chrysochromulima spp. and cyanobacteria species Anabaenopsis spp. also occurred abundantly. The composition of phytoplankton species was different after 20d and 40d incubation germination or in different collection seasons. The germination of resting cells mirrored the phytoplankton composition in the water column to some extent. However, some nannophytoplankton species such as Gymnodinium corii, Chrysochromulima spp. and cyanobacteria species Anabaenopsis spp., occurred abundantly in germinated phytoplankton, were seldom reported in water column in previous phytoplankton surveys, which might be ignored due to their small sizes.
2009 Vol. 29 (12): 1285-1290 [
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1291
Analysis of general additive model on the relationships between chlorophyll-a concentrations and environmental factors in Beihu Lake of Chongming Island.
LIU Jia, HUANG Qing-Hui, LI Jian-Hua
The relationships between chlorophyll-a and its related environmental factors in Beihu lake was studied by GAM method. The total phosphorus, total nitrogen and water temperature were significant as explanatory variables in explaining chlorophyll-a (P<0.05). Chlorophyll-a initially increased monotonically with the total phosphorus, but decreased monotonically after the total phosphorus reached 0.12mg/L. Impacts of different total nitrogen concentrations on chlorophyll-a varied, being weak when the total nitrogen concentration ranged from 0.6 to 1.8mg/L. Highest chlorophyll-a concentrations occurred when the water temperature ranged from 24 to 26℃. Furthermore, the relationship between chlorophyll-a and the TN:TP ratio was significant (P<0.1) nonlinear relationship. Chlorophyll-a was inversely related to TN:TP ratio under nitrogen-limitation. Chlorophyll-a decreased monotonically with TN:TP ratio under phosphorus-limitation.
2009 Vol. 29 (12): 1291-1295 [
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1296
Detection and ambient severity evaluation of organic compounds in Meiliang Bay of Taihu Lake in 2008.
GAO Xiang-Yu, CUI Yi-Bin, HU Chang-Wei, QIAN Xin, LI Mei
The semi-volatile organic compounds in Meiliang Bay of Taihu Lake in different seasons in 2008 were determined qualitativelical and quantitatively by gas chromatography method with mass spectrometric detection. Ambient severity due to exposure to these organic pollutants were evaluated with the multimedia environmental goals (MEG), including both health and ecological ambient severity. Total contents of 25 kinds of organic pollutants from water samples in 4 seasons were 17.459, 11.140, 11.147 and 5.675mg/L, respectively. And phthalate esters, benzenes and polycyclic aromatic hydrocarbons had been determined as high detective rate. The total ambient severity (TAS) of both health and ecology were less than 1, which indicated that water in Meiliang Bay was still safe to health and ecology.
2009 Vol. 29 (12): 1296-1300 [
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1301
Impact of sorption and desorption behaviors of lindane on its sediment quality criteria.
ZHANG Zi-Zhong, CHANG Chun, CHEN Wei, ZHU Ling-Yan
The sorption and desorption of lindane on three sediments were investigated. The sorption isotherms fitted Freundlich model very well. The sorption capacity of sediment was affected by the organic carbon fraction and particle distribution. The desorption isotherms remarkably deviated from the sorption isotherms and displayed a resistant desorption. The desorption results fitted dual equilibrium desorption (DED) model very well. The desorption irreversibility of lindane in sediment increased with its initial concentration in the sediment. The sediment quality criteria (SQC) derived from DED model was 46 times higher than what from linear model.
2009 Vol. 29 (12): 1301-1305 [
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1306
Effect of adding four metal ions on distribution of anthracene on kaolin colloid.
YU Sheng, ZOU Peng, XIAO Lin, YANG Liu-Yan
In order to understand the effect of metal ions on distribution and adsorption of anthracene on soils (water/soil = 10), Fe3+, Cu2+, Zn2+ and Ca2+ were added in the colloid solution of contaminated kaolin to probe the varies of anthracene concentrations in supernatants after 180 r/min of shaking at 28℃. The adsorption equilibrium time and equilibrium concentration of Fe3+, Cu2+, Zn2+ and Ca2+ by kaolin were about 24 h and (20 ± 3) mg/g, respectively. When contents of metal ions added were more than 30 mg/g for kaolin, the lowest concentration of kaolin colloid was (40±3)mg/L in supernatant. The total colloid concentrations in supernatants increased from 52 mg/L to 133 mg/L for adding Fe3+, 52 mg/L to 110 mg/L for adding Cu2+ or Zn2+, and 52 mg/L to 73 mg/L for adding Ca2+, respectively. Meanwhile, the increasing trends of anthracene in the supernatants were similar to those of total colloid concentrations, which were from 30 ng/L to 73 ng/L for adding Fe3+, 28, 35ng/L to 65, 66 ng/L for adding Cu2+ or Zn2+, and 40 ng/L to 50 ng/L for adding Ca2+, respectively. Thus, the sequences of the increase of anthracene in supernatants were the same as those of the charge densities of metal ions (Fe3+ > Cu2+, Zn2+). However, adding Ca2+ at pH 6.5 only led to a minor increase of anthracene in supernatant.
2009 Vol. 29 (12): 1306-1311 [
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1312
Effect of stimulated acid rain on leaching characteristic of elements from waste printed circuit boards in sand column.
ZHAO Guo-Hua, LUO Xing-Zhang, ZHENG Zheng, GAO Shun-Zhi, FENG Jing-Wei, NIE 耳, NI Li-Xiao
Characteristics of elements leaching from waste printed circuit boards (PCBs) was studied through sand column tests. Leaching concentrations of elements from waste PCBs increased greatly with decrease of pH. the alteration of the elements could be categorized into two kinds: one kind included Cu,Pb and Zn, which leaching concentrations could be divided into three periods: fristly quickly increased to the highest,and then abruptly reduced until to the smooth stage; the other kind included Cl,Ca and Mg, which leaching concentrations reached the highest at the initial leaching stages, then abruptly declined to the stable stage. During the smooth stage, the leaching concentrations of Cu and Pb were much higher than standard III of the ground water quality that would result in tremendous impairment to ground water.Moreover, Pb, Zn and Cu in the leachate was simulated by the softwareVisual MINTEQ. The results revealed that Pb2+ and Zn2+were dominant species in the leachate. When pH was below 4.0, Cu2+was the dominant species, while when pH was above 4.0, CuSO4(aq) was the main form.
2009 Vol. 29 (12): 1312-1317 [
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1318
Effects of bisphenol A on the metabolites of zebrafish embryos.
DUAN Zheng-Hua, ZHU Lin, ZHAO Na, BU Wen-Jun, Lam Siew Hong, Gong Zhiyuan, ZOU Li, LI Wei, Ong Eng Shi, Ong Choon Nam
Metabonomics approach based on the GC-MS technique was used to characterize the perturbation of volatile and semi-volatile components in zebrafish embryos induced by bisphenol A (BPA) for 4 d since fertilization. The exposure concentrations of BPA were 0, 0.5, 1.5 and 4.5mg/L, including assumed“safe level”(1.5mg/L) that stated by US EPA. The relative proportions of arachidonic acid and cholesterol were decreased dramatically, and those of saturated fatty acid (SAF), amino acid, glucose and inositol were increased significantly. Therefore, 1.5 mg/L BPA would disturb the regular metabolizing of zebrafish embryos.
2009 Vol. 29 (12): 1318-1322 [
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