Determination of dissolved free amino acids in biogas slurry: RP-HPLC with pre-column derivation
LI Jian-Hua, LIU Wen-Jing, LI Ning
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 20092, China
In this study, a pretreatment protocol was developed, and combined with phthalaldehyde (OPA) and 9-fluorenyl methyl chloroformate (FMOC-Cl) pre-column derivatization, RP-HPLC method, and fluorescent detection for the quantitative and qualitative investigations of dissolved free amino acids (DFAA) in biogas slurry. Owing to the simultaneous removal of high-strength ammonium and biogenic amines by pH adjustment during rotary evaporation, the recovery of DFAA was greatly improved by the introduction of 3K Millipore ultrafiltration tubes. As a result, a good linear relationship (corresponding linear correlation coefficient (R2) greater than 0.99) was obtained with the spike recovery rate of 23 kinds of amino acid of 89%~115%, except for that of glutamic acid of 70.5%. And the relative standard deviation was 1.1%~5.0% for the recovery analysis. On this basis, the developed pre-treatment method was further carried out to different sludge samples. The DFAA was 2.0mol/L in the excess sludge, while the amount of DFAA in the biogas slurry with input TS of 5% and 20% were different in species and amounts with 0.04mol/L and 1.94mol/L, respectively. Thus, no correlation between the DFAA amount and the input had been observed, which proved that the generation and utilization mechanisms of amino acids varied in different anaerobic digesters.
李建华, 刘文静, 李宁. 沼液中溶解游离氨基酸的测定——柱前衍生-反相高效液相色谱法[J]. 中国环境科学, 2016, 36(8): 2355-2363.
LI Jian-Hua, LIU Wen-Jing, LI Ning. Determination of dissolved free amino acids in biogas slurry: RP-HPLC with pre-column derivation. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(8): 2355-2363.
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