Biomineralization of U(VI)-PO43- promoted by Aspergillus tubingensis mediated phytate hydrolysis
WANG Nie-ying1, ZHANG Hui1, SUI Yang1,2, CHEN Yue-yu1, HU Nan1, DAI Zhong-ran1, DING De-xin1
1. Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources, University of South China, Hengyang 421001, China;
2. School of Nuclear and Technology, University of South China, Hengyang 421001, China
The fungus M5-1capable of hydrolyzing phytate was isolated from the sediments of a uranium tailings repository in Guangdong Province. Its colony morphology, ITS sequences, suitable growth pH value, tolerance against uranium and effect on hydrolysis of phytate were systematically studied. The variations of pH value, orthophosphate concentration, uranium concentration and removal efficiency of uranium were monitored. The main elements and mineral components of the biomineralization products were also analyzed. It was found that the strain M5-1was Aspergillus tubingensis (MH978623) with optimal growth at pH 6~7, and with high tolerance against uranium (~0.84mmol/L). After biomineralization of U(VI)-PO43- promoted by Aspergillus tubingensis mediated phytate hydrolysis for 62days, the removal efficiency of uranium reached 95.2%. SEM-EDS and XRD analyses indicated that the insoluble chernikovite and metanatroautunite were formed during the biomineralization of U(VI)-PO43-. The results showed that Aspergillus tubingensis could effectively hydrolyze phytate to release soluble orthophosphate, which promoted the mineralization of U(VI)-PO43-. These results provided an experimental basis for the in-situ bioremediation of uranium contaminated surface water by biomineralization of U(VI)-PO43- promoted by Aspergillus tubingensis mediated phytate hydrolysis.
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