Remediation of uranium contaminated groundwater by β-glycerophosphate
HUANG Chao1,2, ZHANG Hui1,2, HU Nan1,2, DING De-xin1,2, LI Dian-xin1,2, SUN Jing1,2
1. Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China;
2. Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources, University of South China, Hengyang 421001, China
The microcosm experiment were conducted to investigate the remediation effect of uranium contaminated groundwater by β-glycerophosphate. The variations of pH and nitrate, sulfate, phosphate, and uranium concentrations were monitored during the incubation, and the uranium species and the proportion of U(IV) in sediments were analysed. It was found that, after 25d incubation, the concentrations of uranium in liquids in the β-glycerophosphate and glycerol amended groups decreased from 2.96 and 2.99mg/L to 0.030 and 0.044mg/L, respectively, which were lower than the limits of contaminants stipulated in the regulations on radiation and environment protection in uranium mining and milling in China. β-glycerophosphate worked as the carbon and phosphate sources and reduced the proportion of the uranium species in exchangeable and carbonate fractions in sediments and increased the proportion of the uranium species in Fe(Mn) oxides, organic matter and residual fractions in sediments. The proportion of U(IV) in the sediment of β-glycerophosphate amended group amounted to 91.79%, which was 28.82% higher than that of the glycerol amended group. After 45d anaerobic incubation, the proportion of U(IV) in the sediment of β-glycerophosphate amended group almost remained unchanged, while that in glycerol amended group decreased by 7.98%. Therefore, β-glycerophosphate could work as carbon and phosphate sources and could immobilize uranium in-situ through bioreduction and biomineralization.
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