Effect of ANAMMOX enrichment and optimization of non-aeration/aeration ratio in MBR-SNAD process
ZHANG Kai1, ZHANG Zhi-hua2, WANG Zhao-zhao3, LI Jun1, HOU Lian-gang1, LIANG Dong-bo1, DING Fan1
1. National Engineering Laboratory of Urban Sewage Advanced Treatment and Resource Utilization Technology, The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China;
2. Xingtai Medical College, Xingtai 054000, China;
3. College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China
The activity changes of anaerobic ammonia-oxidizing bacteria during the enrichment process was studied based on the membrane bioreactor (MBR). Completely autotrophic nitrogen removal over nitrite (CANON) process was started up by constant aeration rate. Simultaneous denitrification of ammonia oxidizing bacteria (AerAOB) and anaerobic ammonia oxidizing bacteria (AnAOB) by optimizing the intermittent aeration and effectively inhibiting the activity of nitrite oxidizing bacteria (NOB), then the simultaneous nitrification, anammox and denitrification (SNAD) process was started up successfully by adding the the carbon source (sodium acetate). The results showed that enhanced the activity of anaerobic ammonia-oxidizing bacteria during the enrichment process of anaerobic ammonia-oxidizing bacteria by continuously shortening the hydraulic retention time (HRT) and increasing influent nitrogen loading. The average activity of the anaerobic ammonia-oxidizing bacteria increased from 0.603mgN/(h·gVSS) to 8.1mgN/(h·gVSS). When the constant aeration rate was 50mL/min and the intermittent aeration (non-aeration time:aeration time, mim/min) was 4:10, the removal of ammonium by AerAOB and AnAOB accounted for 58.8% and 41.2% of the total ammonium removal respectively, and the amount of nitrite nitrogen oxidized by NOB accounted for 15.3% of the total nitrate nitrogen production, successfully inhibiting the activity of NOB. When the C/N ratio was 0.5 and the intermittent aeration was adjusted to 4:15, the nitrogen removal rate in the denitrification process accounted for 20.9% of the total nitrogen removal rate, and the nitrogen removal rate in the anaerobic ammonia oxidation process accounted for 79.1% of the total nitrogen removal rate. The purpose of synergistic denitrification of AerAOB, AnAOB and denitrifying bacteria (DNB) was achieved finally.
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