一种浮游植物快速浓缩及固定方法的可靠性分析

李亚莉; 杨正健; 田盼; 刘毅; 王从锋; 纪道斌; 刘德富

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中国环境科学 ›› 2021, Vol. 41 ›› Issue (9) : 4300-4309.

环境生态

一种浮游植物快速浓缩及固定方法的可靠性分析

李亚莉¹, 杨正健^1,2, 田盼¹, 刘毅¹, 王从锋¹, 纪道斌¹, 刘德富²

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Reliability analysis of a method to rapidly concentrate and fix phytoplankton

LI Ya-li¹, YANG Zheng-jian^1,2, TIAN Pan¹, LIU Yi¹, WANG Cong-feng¹, JI Dao-bin¹, LIU De-fu²

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摘要

为验证一种浮游植物快速浓缩固定方法——滤膜浓缩法的可靠性，采用“鲁戈氏液固定沉降法”和“滤膜浓缩法”同时鉴定了不同浮游植物浓度梯度水样，并进行了对比分析，结果表明：鲁戈氏液固定沉降法与滤膜浓缩法整体上均鉴定出7门，32属，但不同梯度上滤膜浓缩法鉴定出的浮游植物种类较多.两种方法门水平上除裸藻门（P<0.05）外丰度均无明显差异（P>0.05），属水平上相对丰度99%以内的浮游植物均无明显差异（P>0.05），即无论是门还是属水平上，滤膜浓缩法对浮游植物丰度的鉴定是可行的.采用两种方法得到的不同梯度下藻密度拟合方程斜率差异较小（P>0.05）且相关性显著（R²=0.958，P<0.01），但滤膜浓缩法鉴定出的浮游植物细胞密度更大.整体来看，滤膜浓缩法得到的藻类鉴定结果与鲁戈氏液固定沉降法基本一致.

Abstract

In order to verify the reliability of a membrane concentration method to rapidly concentrate and fixe phytoplankton in the field, the "Lugol's solution fixed sedimentation method (LSM)" and "membrane concentration method (MCM)" were comparatively used here to authenticate different phytoplankton samples. The results show that:(1) 7 phyla and 32 genera were both identified by the two method, but using the MCM could authenticate more phytoplankton species than the LSM method; (2) There was no significant difference not only in phytoplankton abundance between the two methods except Euglenophyta (P<0.05) at the phylum level (P>0.05), but also in phytoplankton relative abundance within 99% at the genus level (P>0.05). Which meant that, at the phylum and genus levels, the MCM was feasible to identify the phytoplankton abundance; (3) There was no significant difference in the slope of fitting equation of different algae biomass gradients (P>0.05), and they showed a high correlation coefficient (R²=0.958, P<0.01), however, algal biomass of the MCM seemed to be a little larger. On a conclusion, the results of the MCM was consistent with the LSM, and the MCM had more advantages in less environmental condition requirement, easier for field monitoring, and save time.

导出引用

李亚莉, 杨正健, 田盼, 刘毅, 王从锋, 纪道斌, 刘德富. 一种浮游植物快速浓缩及固定方法的可靠性分析[J]. 中国环境科学. 2021, 41(9): 4300-4309

LI Ya-li, YANG Zheng-jian, TIAN Pan, LIU Yi, WANG Cong-feng, JI Dao-bin, LIU De-fu. Reliability analysis of a method to rapidly concentrate and fix phytoplankton[J]. China Environmental Science. 2021, 41(9): 4300-4309

中图分类号： X52 X171.5

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