Microbial photoelectric reduction of CO2 to acetate and its response mechanism to external applied voltage
ZHOU Mei-zhou1, LUO Hai-ping1, ZENG Cui-ping2, LIU Guang-li1, ZHANG Ren-duo1
1. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China; 2. Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Abstract：For evaluating the performance of microbial photoelectric synthesis (MPES) to reduce CO2 to synthesize acetic acid and its limiting factors, this study tried to construct a novel double-chamber microbial photo-electrosynthesis system (MPES)by coupling TiO2 photoanode with biocathode and using solar energy as main energy. The replacement of pure electrochemical anodes by photoanodes significantly reduced the external voltage requirements of MPES biocathodes, and MPES could continue to operate stably, with an average acetic acid production rate of (1.18 ±0.11) mmol/(L·d) and a Faraday efficiency of 45.75% ±3.97%. The photoanode drives the cathode to produce hydrogen, suggesting that the cathodic microorganisms tend to use H2-mediated electron transfer. The external voltage influenced the performance of the MPES significantly by affecting the electron donating ability of the photoanode. When the external voltage was increased from 0.4~0.6V, the MPES current, acetate production and Faraday efficiency were significantly improved, and the performance of the MPES was mainly limited by the photoanode. When the external voltage was higher than 0.6V, the system current and the output of acetic acid increased mildly, and Faraday efficiency reached the maximum value at 0.8V, and then declined, indicating that the electron-acceptting ability of biocathode was saturated at 0.8V and the performance of the MPES was mainly limited by the biocathode. As an electron intermiate, H2 was incompletely utilized during the operation of MPES, explaining why the Faraday efficiency was not further improved with an increase in external voltage.
周美洲, 骆海萍, 曾翠平, 刘广立, 张仁铎. 微生物光电还原CO2合成乙酸对外电压的响应机制[J]. 中国环境科学, 2022, 42(2): 907-913.
ZHOU Mei-zhou, LUO Hai-ping, ZENG Cui-ping, LIU Guang-li, ZHANG Ren-duo. Microbial photoelectric reduction of CO2 to acetate and its response mechanism to external applied voltage. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 907-913.
Nevin K P, Woodard T L, Franks A E, et al. Microbial electrosynthesis:feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic compounds[J]. mBio., 2010,1(2):e103-e110.
Logan B E, Rossi R, Ragab A, et al. Electroactive microorganisms in bioelectrochemical systems[J]. Nature Reviews Microbiology, 2019, 17(5):307-319.
Saratale R G, Saratale G D, Pugazhendhi A, et al. Microbiome involved in microbial electrochemical systems (MESs):A review[J]. Chemosphere, 2017,177(6):176-188.
张尧,张闻杰,蒋永,等.生物电化学系统固定二氧化碳同时产生乙酸和丁酸[J]. 应用与环境生物学报, 2014,20(2):174-178. Zhang R, Zhang W, Jiang Y, et al. Simultaneous microbial electrosynthesis of acetate and butyrate from carbon dioxide in bioelectrochemical systems[J]. Chinese Journal of Applied and Environmental Biology, 2014,20(2):174-178.
Chu N, Liang Q, Jiang Y, et al. Microbial electrochemical platform for the production of renewable fuels and chemicals[J]. Biosensors and Bioelectronics, 2020,150(2):111922.
Bajracharya S, Srikanth S, Mohanakrishna G, et al. Biotransformation of carbon dioxide in bioelectrochemical systems:State of the art and future prospects[J]. Journal of Power Sources, 2017,356(7):256-273.
Marshall C W, Ross D E, Fichot E B, et al. Long-term operation of microbial electrosynthesis systems improves acetate production by autotrophic microbiomes[J]. Environmental Science & Technology, 2013,47(11):6023-6029.
Yang Y, Niu S, Han D, et al. Progress in developing metal oxide nanomaterials for photoelectrochemical water splitting[J]. Advanced Energy Materials, 2017,7(19):1-26.
Liu C, Gallagher J J, Sakimoto K K, et al. Nanowire-bacteria hybrids for unassisted solar carbon dioxide fixation to value-added chemicals[J]. Nano Letters, 2015,15(5):3634-3639.
Bian B, Shi L, Katuri K P, et al. Efficient solar-to-acetate conversion from CO2 through microbial electrosynthesis coupled with stable photoanode[J]. Applied Energy, 2020,278(10):115684.
Bai Y, Mora-Seró I, De Angelis F, et al. Titanium dioxide nanomaterials for photovoltaic applications[J]. Chemical Reviews, 2014,114(19):10095-10130.
Chen X, Mao S S. Titanium dioxide nanomaterials:Synthesis, properties, modifications, and applications[J]. Chemical Reviews, 2007,107(7):2891-2959.
Luo C, Ren X, Dai Z, et al. Present Perspectives of Advanced Characterization Techniques in TiO2-Based Photocatalysts[J]. ACS Applied Materials & Interfaces, 2017,9(28):23265-23286.
Cheng J, Zhang M, Wu G, et al. Photoelectrocatalytic Reduction of CO2 into Chemicals Using Pt-Modified Reduced Graphene Oxide Combined with Pt-Modified TiO2 Nanotubes[J]. Environmental Science & Technology, 2014,48(12):7076-7084.
Liu B, Aydil E S. Growth of oriented single-crystalline rutile TiO2 nanorods on transparent conducting substrates for dye-sensitized solar cells[J]. Journal of the American Chemical Society, 2009,131(11):3985-3990.
Hou Y, Luo H, Liu G, et al. Improved hydrogen production in the microbial electrolysis cell by inhibiting methanogenesis using ultraviolet irradiation[J]. Environmental Science & Technology, 2014,48(17):10482-10488.
Xiang Y, Liu G, Zhang R, et al. Acetate production and electron utilization facilitated by sulfate-reducing bacteria in a microbial electrosynthesis system[J]. Bioresource Technology, 2017,241(10):821-829.
Chiranjeevi P, Patil S A. Strategies for improving the electroactivity and specific metabolic functionality of microorganisms for various microbial electrochemical technologies[J]. Biotechnology Advances, 2020,39(3/4):107468.
Patil S A, Arends J B A, Vanwonterghem I, et al. Selective enrichment establishes a stable performing community for microbial electrosynthesis of acetate from CO2[J]. Environmental Science & Technology, 2015,49(14):8833-8843.
Bajracharya S, ter Heijne A, Dominguez Benetton X, et al. Carbon dioxide reduction by mixed and pure cultures in microbial electrosynthesis using an assembly of graphite felt and stainless steel as a cathode[J]. Bioresource Technology, 2015,195(11):14-24.
Gildemyn S, Verbeeck K, Slabbinck R, et al. Integrated production, extraction, and concentration of acetic acid from CO2 through microbial electrosynthesis[J]. Environmental Science & Technology Letters, 2015,2(11):325-328.
Mohanakrishna G, Vanbroekhoven K, Pant D. Imperative role of applied potential and inorganic carbon source on acetate production through microbial electrosynthesis[J]. Journal of CO2 Utilization, 2016,15(9):57-64.
Bajracharya S, Yuliasni R, Vanbroekhoven K, et al. Long-term operation of microbial electrosynthesis cell reducing CO2 to multi-carbon chemicals with a mixed culture avoiding methanogenesis[J]. Bioelectrochemistry, 2017,113(2):26-34.
Xiang Y, Liu G, Zhang R, et al. High-efficient acetate production from carbon dioxide using a bioanode microbial electrosynthesis system with bipolar membrane[J]. Bioresource Technology, 2017, 233(6):227-235.
Nevin K P, Hensley S A, Franks A E, et al. Electrosynthesis of organic compounds from carbon dioxide is catalyzed by a diversity of acetogenic microorganisms[J]. Applied and Environmental Microbiology, 2011,77(9):2882-2886.
Batlle-Vilanova P, Ganigué R, Ramió-Pujol S, et al. Microbial electrosynthesis of butyrate from carbon dioxide:Production and extraction[J]. Bioelectrochemistry, 2017,117(6):57-64.
Fu Q, Xiao S, Li Z, et al. Hybrid solar-to-methane conversion system with a Faradaic efficiency of up to 96%[J]. Nano Energy, 2018,53(8):232-239.
Xiao S, Li Z, Fu Q, et al. Hybrid microbial photoelectrochemical system reduces CO2 to CH4 with 1.28% solar energy conversion efficiency[J]. Chemical Engineering Journal, 2020,390(2):124530.
Tremblay P, Angenent L T, Zhang T. Extracellular electron uptake:among autotrophs and mediated by surfaces[J]. Trends in Biotechnology, 2017,35(4):360-371.
Marshall C W, Ross D E, Handley K M, et al. Metabolic reconstruction and modeling microbial electrosynthesis[J]. Scientific Reports, 2017,7(1):8391.
Kumar A, Hsu L H, Kavanagh P, et al. The ins and outs of microorganism-electrode electron transfer reactions[J]. Nature Reviews Chemistry, 2017,1(3):5181-5192.
Qiao J, Liu Y, Hong F, et al. A review of catalysts for the electroreduction of carbon dioxide to produce low-carbon fuels[J]. Chemical Society reviews, 2013,43(2):631-675.
Luo H, Qi J, Zhou M, et al. Enhanced electron transfer on microbial electrosynthesis biocathode by polypyrrole-coated acetogens[J]. Bioresource Technology, 2020,309(4):123322.
李明玉,尚薇,王心乐,等.光电化学协同催化降解甲基橙的研究[J]. 中国环境科学, 2009,29(5):512-517. Li M, Shang W, Wang X, et al. The degradation of methyl orange with photo-electro-chemical synergistic catalysis system[J]. China Environmental Science, 2009,29(5):512-517.
Irtem E, Hernández-Alonso M D, Parra A, et al. A photoelectrochemical flow cell design for the efficient CO2 conversion to fuels[J]. Electrochimica Acta, 2017,240(6):225-230.
Fu Q, Kuramochi Y, Fukushima N, et al. Bioelectrochemical analyses of the development of a thermophilic biocathode catalyzing electromethanogenesis[J]. Environmental Science & Technology, 2015,49(2):1225-1232.