鲴鲢鳙混养系统中微生物对氮素迁移转化的影响

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摘要通过实验室模拟富营养化水体进行鲢（Hypophthalmichthys molitrix）鳙（Aristichthys nobilis）鲴（Plagiogathops micrloepis Bleeker）混养，结合同位素标记与微生物检测，研究其中沉积物微生物群落的变化及其对氮素迁移转化的影响，揭示鲢鳙鲴混养作用机制.结果表明，鲢鳙鲴混养增加了沉积物中氨化细菌（芽孢杆菌属与假单胞菌属）的数量，实验结束时，与其它组相比，鲢鳙鲴组的芽孢杆菌属（Bacillus）与假单胞菌属（Pseudomonas）总占比最高，为31.2%，这增强了氨化作用，减少了沉积物中有机氮的含量，使得鲢鳙鲴组的δ¹⁵N （底质沉积物）在实验结束时降至726.8‰.鲢鳙鲴混养提高了沉积物中反硝化菌属（动胶菌属与芽胞杆菌属）的数量，实验结束，鲢鳙鲴组动胶菌属（Zoogloea）与芽胞杆菌属（Bacillus）占比显著高于鲢鳙组（P<0.05），达到8.17%、33.7%，使得系统中反硝化反应加强，使水体中更多的硝酸盐氮转化为气态氮并移出水体，实验结束时，与其它组相比，鲢鳙鲴组δ¹⁵N_{（硝酸盐氮）}降低至96.3‰，¹⁵N总含量也降低至6.94μmol.

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	张哲
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	张毅敏
	陈玲玲
	郭艳敏
	朱月明
	于江华

关键词 ：鲢鳙鲴, 混养, 稳定同位素技术, 氮素, 微生物群落

Abstract：The polyculture system of Plagiogathops microlepis Bleeker (Xenocypris microlepis), Hypophthalmichthys molitrix (Silver carp) and Aristichthys nobilis (bighead carp) was set up in simulated eutrophic water. The dynamics of microbial communities and their effects on the migration and transformation of nitrogen in the sediments were investigated by applying isotopic labeling and microbial analysis to reveal the underlying mechanism of the polyculture system. The results showed that:The polyculture of Xenocypris microlepis, silver carp and bighead carp could increase the proportion of Pseudomonas and Bacillus involved in the ammonification process in the sediments. Comparing with the silver carp and bighead carp group, the total proportion of Bacillus and Pseudomonas in the polyculture group was the highest (31.2%) at the end of experiment, showing that it enhanced ammonification process and thus, reduced the content of organic nitrogen in the sediments. Consequently, the δ¹⁵N_{(substrate sediment)} of the polyculture group decreased to 726.8‰. The polyculture of Xenocypris microlepis, silver carp and bighead carp could increase the proportion of denitrifying bacteria (Zoogloea and Bacillus) in the sediments. The proportion of Zoogloea and Bacillus in the polyculture group was significantly higher than that in the silver carp and bighead carp group (P<0.05), reaching 8.17% and 33.7%, respectively. Therefore, the denitrification process was enhanced in the system, converting more nitrate into nitrogen gas in the water and finally releasing this gas from this water. At the end of the experiment, in the polyculture group, the δ¹⁵N_{(nitrate nitrogen)}decreased to 96.3‰ and the total content of ¹⁵N decreased to 6.94μmol.

Key words： Plagiogathops microlepis Bleeker, Hypophythalmichthys molitrix and Aristichthys nobilis polyculture stable isotope technique nitrogen microbial community

收稿日期: 2021-05-12

PACS:

X524

基金资助:国家水体污染控制与治理重大专项（2017ZX07202006）；中央级公益性科研院所基本科研业务专项（GYZX200204）

通讯作者: 张毅敏,研究员,zym7127@163.com E-mail: zym7127@163.com

作者简介: 张哲(1996-),男,山东德州人,南京信息工程大学硕士研究生,主要从事水环境治理和流域生态保护研究.发表论文2篇.

引用本文:

张哲, 高月香, 张毅敏, 陈玲玲, 郭艳敏, 朱月明, 于江华. 鲴鲢鳙混养系统中微生物对氮素迁移转化的影响[J]. 中国环境科学, 2022, 42(2): 897-906. ZHANG Zhe, GAO Yue-xiang, ZHANG Yi-min, CHENG Ling-ling, GUO Yan-min, ZHU Yue-ming, YU Jiang-hua. The effects of microorganisms on the migration and transformation of nitrogen in the polyculture system of Plagiogathops microlepis Bleeker, Hypophthalmichthys molitrix and Aristichthys nobilis. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 897-906.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I2/897

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